FastQCFastQC Report
Thu 26 May 2016
SRR1546639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546639_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1270172
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39470.3107453163823482No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36140.28452839457963175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14530.11439395609413529No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATG650.044.0000041
CCTTACG207.856795E-444.01
TCGTTAG950.044.01
ACGCCGA254.4431054E-544.012
CACGGCT207.856795E-444.021
CGTGCGC302.5279278E-644.033
CTATCGA302.5279278E-644.01
GCGCGCG207.856795E-444.01
CGGTTCG207.856795E-444.029
TAGCGCA254.4431054E-544.027
TCGGGCA302.5279278E-644.05
TGAACCG207.856795E-444.034
TGCGGTG351.4462421E-744.04
CTAAGCG302.5279278E-644.01
CACGAGA254.4431054E-544.022
TCGATTG408.314601E-944.01
CGTTCCG502.7284841E-1144.040
ATCGTAC351.4462421E-744.026
CCCGTAT408.314601E-944.034
CCGTACA502.7284841E-1144.026
Produced by FastQC (version 0.11.5)