FastQCFastQC Report
Thu 26 May 2016
SRR1546638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546638_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1802800
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88100.48868426891502104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCG600.044.01
AGTGTCG207.8576757E-444.01
CGTTTTT84350.042.1742741
GAGTACG750.041.0666661
TCGTAAG750.041.0666661
TATAACG750.041.0666661
TACGGGA9600.039.6458324
GTTAGCG1000.039.61
ACGGGAT12950.039.2432445
CGTTAGG3150.039.111112
ACGGGTA800.038.55
TTACGCG404.1262865E-738.51
AATACGG1550.038.322582
CGTAAGG2250.038.1333352
CTAAGCG750.038.133331
GGACCGA11800.037.6610158
AAGGGAT48650.037.442965
GGGACCG13350.037.4082377
GACCGAT10900.037.339459
AGGGATT63450.037.1347546
Produced by FastQC (version 0.11.5)