##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546638_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1802800
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.997487242067894	31.0	31.0	33.0	30.0	34.0
2	31.376207566008432	31.0	31.0	34.0	30.0	34.0
3	31.51797592633681	31.0	31.0	34.0	30.0	34.0
4	35.286733414688264	37.0	35.0	37.0	33.0	37.0
5	35.08966274683825	37.0	35.0	37.0	32.0	37.0
6	35.168207787885514	37.0	35.0	37.0	32.0	37.0
7	35.74629687153317	37.0	35.0	37.0	35.0	37.0
8	35.799251164854674	37.0	35.0	37.0	35.0	37.0
9	37.55533392500555	39.0	37.0	39.0	35.0	39.0
10	36.78402817838917	39.0	37.0	39.0	32.0	39.0
11	36.362368537830044	38.0	35.0	39.0	32.0	39.0
12	36.02105835367207	38.0	35.0	39.0	31.0	39.0
13	35.916449412025734	38.0	35.0	39.0	30.0	39.0
14	36.89397770135345	39.0	35.0	41.0	31.0	41.0
15	37.0581856001775	39.0	35.0	41.0	31.0	41.0
16	37.07645662303084	39.0	35.0	41.0	32.0	41.0
17	36.97375027734635	38.0	35.0	41.0	31.0	41.0
18	36.88077157754604	38.0	35.0	40.0	31.0	41.0
19	36.85535167517195	38.0	35.0	40.0	31.0	41.0
20	36.711483802973156	38.0	35.0	40.0	31.0	41.0
21	36.62105058797426	38.0	35.0	40.0	31.0	41.0
22	36.550914133570004	38.0	35.0	40.0	30.0	41.0
23	36.36368704237852	38.0	35.0	40.0	30.0	41.0
24	36.3352235411582	38.0	35.0	40.0	30.0	41.0
25	36.20543820723319	38.0	35.0	40.0	30.0	41.0
26	36.09744785888618	38.0	34.0	40.0	30.0	41.0
27	35.94054470823164	38.0	34.0	40.0	30.0	41.0
28	35.896172065675614	38.0	34.0	40.0	29.0	41.0
29	35.776718992678056	38.0	34.0	40.0	29.0	41.0
30	35.743718105169734	38.0	34.0	40.0	29.0	41.0
31	35.681163190592414	38.0	34.0	40.0	29.0	41.0
32	35.46701187042378	38.0	34.0	40.0	27.0	41.0
33	35.23829709341025	38.0	34.0	40.0	26.0	41.0
34	35.157578766363436	38.0	34.0	40.0	26.0	41.0
35	34.94277568227202	38.0	34.0	40.0	25.0	41.0
36	34.769466385622366	38.0	33.0	40.0	24.0	41.0
37	34.851081096072775	38.0	34.0	40.0	24.0	41.0
38	34.80097570445973	38.0	33.0	40.0	24.0	41.0
39	34.71757211005103	38.0	33.0	40.0	24.0	41.0
40	34.74260261814955	38.0	34.0	40.0	24.0	41.0
41	34.77801420013313	38.0	34.0	40.0	24.0	41.0
42	34.55019247836699	38.0	33.0	40.0	23.0	41.0
43	34.61861382294209	38.0	33.0	40.0	23.0	41.0
44	34.45455680053251	38.0	33.0	40.0	23.0	41.0
45	34.27508375859774	37.0	33.0	40.0	23.0	41.0
46	34.380690037719106	37.0	33.0	40.0	23.0	41.0
47	34.25474650543599	37.0	33.0	40.0	23.0	41.0
48	34.3140964055913	37.0	33.0	40.0	23.0	41.0
49	34.23897381850455	37.0	33.0	40.0	23.0	41.0
50	33.76968659862436	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	3.0
14	13.0
15	49.0
16	151.0
17	387.0
18	915.0
19	1869.0
20	3204.0
21	5119.0
22	7760.0
23	11383.0
24	16087.0
25	22831.0
26	30577.0
27	36358.0
28	40622.0
29	46173.0
30	54410.0
31	64859.0
32	79586.0
33	97403.0
34	138194.0
35	176366.0
36	149428.0
37	184820.0
38	260787.0
39	373444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.634679387619258	24.24367650321722	29.18643221655203	14.935211892611495
2	29.883625471488795	26.268193920568006	30.41757266474373	13.430607943199469
3	26.745285112047924	26.35899711559796	33.42727978699801	13.468437985356113
4	23.21854892389616	29.531284668293768	32.96083869536277	14.289327712447303
5	20.988906145995117	33.78178389172399	32.120368315952966	13.108941646327935
6	20.78627690259596	41.65775460394941	27.900488129576217	9.655480363878413
7	90.03389172398492	2.677168848457954	6.406700687818949	0.882238739738185
8	90.9070335034391	1.96588639893499	6.054359884623919	1.0727202130019968
9	84.82039050366097	5.642223208342578	7.5130907477257605	2.02429554027069
10	34.93959396494342	44.39410916352341	12.358664299977812	8.307632571555358
11	25.092522742400707	25.892944308852893	34.26963612158864	14.744896827157755
12	26.022076769469717	24.801309074772576	32.642888839582874	16.533725316174838
13	20.646827157754604	30.858165076547593	33.005602396272465	15.489405369425338
14	17.77507211005103	33.31900377191036	32.22043487907699	16.685489238961615
15	16.136398934990016	27.08259374306634	40.44619480807633	16.334812513867316
16	19.1518193920568	25.714277790104283	38.93560017750166	16.198302640337253
17	19.117650321721765	24.890059906811626	32.9904592855558	23.001830485910805
18	19.743621033947193	25.893332593743068	35.97115597958731	18.39189039272243
19	23.32943199467495	25.89150210783226	32.48530064344353	18.29376525404926
20	24.58436875970712	26.895218548923893	31.122198801863764	17.398213889505215
21	21.839527401819392	28.162857776791654	32.81040603505658	17.187208786332373
22	21.76031728422454	25.665242955402707	31.685711116041716	20.88872864433104
23	18.21865986243621	29.66313512314178	32.16036165964056	19.957843354781453
24	17.963889505214112	27.184490792101172	37.56789438650988	17.283725316174838
25	17.7570445972931	28.362658087419568	35.698857333037495	18.181439982249834
26	17.953072997559353	31.96965830929665	31.94270024406479	18.13456844907921
27	17.906256933658753	31.55724428666519	32.49944530729976	18.037053472376304
28	17.059851342356335	29.331650765475924	36.242178832926555	17.36631905924118
29	17.21300199689372	26.75327268693144	36.540270690037715	19.49345462613712
30	18.327878855114264	29.98075216330153	33.588917239849124	18.102451741735077
31	22.317783447969823	28.743787441757267	30.90819835810961	18.030230752163302
32	22.88784113601065	28.61393388063013	31.529121366762812	16.969103616596406
33	22.33398047481695	28.633348125138674	30.812070113157308	18.220601286887064
34	18.970878633237188	29.129576214777014	31.304082538273796	20.595462613712005
35	18.695529176836033	28.534834701575328	32.91429997781229	19.85533614377635
36	23.063013090747724	28.20928555580209	30.291491013978256	18.43621033947193
37	19.564843576658532	31.19880186376747	31.383570002218768	17.852784557355225
38	19.786443310406035	31.411471045041043	29.63040825382738	19.171677390725538
39	19.73413578877302	31.71172620368316	30.162136676281342	18.39200133126248
40	21.84745950743288	28.856168182826714	29.96233636565343	19.334035944086974
41	18.342689150210784	28.588251608608832	30.731306856001776	22.33775238517861
42	19.860328378078545	28.332593743066344	29.803805191923676	22.003272686931442
43	19.740071000665633	28.56922564899046	30.526015087641444	21.164688262702462
44	18.969713778566675	30.58736410028844	31.107388506767254	19.335533614377635
45	18.481306856001776	32.597736853783005	29.371865986243623	19.5490903039716
46	19.83131794985578	31.60583536720657	29.65575771022853	18.90708897270912
47	19.66768360328378	29.25438207233193	30.11770579099179	20.9602285333925
48	19.733525626802752	28.473430219658308	32.268970490348345	19.524073663190592
49	19.793543376969158	27.908420235189705	32.62242067894386	19.675615708897272
50	19.177834479698248	31.648879520745503	30.089471932549365	19.08381406700688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1337.0
1	1605.0
2	1873.0
3	9646.0
4	17419.0
5	13487.5
6	9556.0
7	9814.0
8	10072.0
9	10523.5
10	10975.0
11	11167.5
12	11360.0
13	11331.5
14	11303.0
15	11292.0
16	11281.0
17	10815.0
18	10349.0
19	10528.0
20	10707.0
21	12480.5
22	14254.0
23	16650.0
24	19046.0
25	22405.5
26	25765.0
27	31862.0
28	37959.0
29	43434.0
30	48909.0
31	58080.5
32	67252.0
33	72856.5
34	78461.0
35	87950.0
36	97439.0
37	101968.0
38	106497.0
39	117101.5
40	127706.0
41	137622.5
42	147539.0
43	156850.0
44	166161.0
45	161494.5
46	156828.0
47	149346.0
48	141864.0
49	129760.0
50	117656.0
51	106253.0
52	94850.0
53	83050.5
54	71251.0
55	61573.5
56	51896.0
57	45421.0
58	38946.0
59	34980.5
60	31015.0
61	26452.0
62	21889.0
63	17817.5
64	13746.0
65	11260.0
66	8774.0
67	6868.0
68	4962.0
69	3712.0
70	2462.0
71	1898.5
72	1335.0
73	1108.0
74	881.0
75	756.5
76	632.0
77	465.5
78	299.0
79	217.5
80	136.0
81	120.0
82	104.0
83	71.0
84	38.0
85	23.5
86	9.0
87	7.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1802800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.028992258142445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21719002614799	18.314222690756818
2	6.204693711791265	2.9818507432955483
3	2.5651155910518453	1.8491142803587581
4	1.7136014101069168	1.6470446006800434
5	1.253386613607513	1.5058808617417154
6	0.9889301312719743	1.4257796680906838
7	0.8430668828997221	1.4180633321605594
8	0.6944067292774116	1.3348715137447154
9	0.6053203940962288	1.3090715157090613
>10	7.461451273040474	40.867608850779426
>50	1.2482146613234653	20.380602148388384
>100	0.2039210124829085	6.323268569583103
>500	0.0	0.0
>1k	4.6770860149163515E-4	0.1475623763633789
>5k	2.3385430074581757E-4	0.4950588483478173
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8810	0.48868426891502104	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027179942311959176	0.0
2	0.0	0.0	0.0	0.0976259152429554	0.0
3	0.0	0.0	0.0	0.1464943421344575	0.0
4	0.0	0.0	0.0	0.22753494564011537	0.0
5	0.0	0.0	0.0	0.3643221655203018	0.0
6	0.0	0.0	0.0	0.5955180829820279	0.0
7	0.0	0.0	0.0	0.7121144885733304	0.0
8	0.0	0.0	0.0	1.0041047259818061	0.0
9	0.0	0.0	0.0	1.1482693587752386	0.0
10	0.0	0.0	0.0	1.3729199023740848	0.0
11	0.0	0.0	0.0	1.5526958065231862	0.0
12	0.0	0.0	0.0	1.7177723541158199	0.0
13	0.0	0.0	0.0	1.8153982693587751	0.0
14	0.0	0.0	0.0	1.8658198358109608	0.0
15	0.0	0.0	0.0	1.9138007543820723	0.0
16	0.0	0.0	0.0	1.9993343687597072	0.0
17	0.0	0.0	0.0	2.1155979587308633	0.0
18	0.0	0.0	0.0	2.26309074772576	0.0
19	0.0	0.0	0.0	2.3394719325493676	0.0
20	0.0	0.0	0.0	2.4220102063456843	0.0
21	0.0	0.0	0.0	2.5229642777901042	0.0
22	0.0	0.0	0.0	2.639449744841358	0.0
23	0.0	0.0	0.0	2.76913689815842	0.0
24	0.0	0.0	0.0	2.873918349234524	0.0
25	0.0	0.0	0.0	2.9623918349234524	0.0
26	0.0	0.0	0.0	3.0529731528733084	0.0
27	0.0	0.0	0.0	3.1484357665853118	0.0
28	4.992234302196583E-4	0.0	0.0	3.244952296427779	0.0
29	4.992234302196583E-4	0.0	0.0	3.3540048812957624	0.0
30	4.992234302196583E-4	0.0	0.0	3.5031062791213667	0.0
31	4.992234302196583E-4	0.0	0.0	3.631406700687819	0.0
32	4.992234302196583E-4	0.0	0.0	3.750388284890171	0.0
33	4.992234302196583E-4	0.0	0.0	3.8700355003328157	0.0
34	4.992234302196583E-4	0.0	0.0	3.9896827157754604	0.0
35	4.992234302196583E-4	0.0	0.0	4.13218327046816	0.0
36	5.546927002440647E-4	0.0	0.0	4.268138451297981	0.0
37	5.546927002440647E-4	0.0	0.0	4.420235189704903	0.0
38	5.546927002440647E-4	0.0	0.0	4.569558464610606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTGCG	60	0.0	44.0	1
AGTGTCG	20	7.8576757E-4	44.0	1
CGTTTTT	8435	0.0	42.174274	1
GAGTACG	75	0.0	41.066666	1
TCGTAAG	75	0.0	41.066666	1
TATAACG	75	0.0	41.066666	1
TACGGGA	960	0.0	39.645832	4
GTTAGCG	100	0.0	39.6	1
ACGGGAT	1295	0.0	39.243244	5
CGTTAGG	315	0.0	39.11111	2
ACGGGTA	80	0.0	38.5	5
TTACGCG	40	4.1262865E-7	38.5	1
AATACGG	155	0.0	38.32258	2
CGTAAGG	225	0.0	38.133335	2
CTAAGCG	75	0.0	38.13333	1
GGACCGA	1180	0.0	37.661015	8
AAGGGAT	4865	0.0	37.44296	5
GGGACCG	1335	0.0	37.408237	7
GACCGAT	1090	0.0	37.33945	9
AGGGATT	6345	0.0	37.134754	6
>>END_MODULE