FastQCFastQC Report
Thu 26 May 2016
SRR1546637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546637_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1654904
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74490.4501167439319743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA207.8574877E-444.024
TCGAACG302.5283953E-644.01
CGTTTTT60650.040.953011
CGCATCG501.3478711E-939.621
CGTTAGG1700.038.8235322
CGTAAGG3600.038.52
TAACGGG4150.038.1686743
ACGGGAC3650.037.97265
TACGGGA4350.037.9310344
AGGGATT28700.037.7909436
TCGTAGC357.2921484E-637.71428730
GGCGATA9550.037.0890058
AGGGCGA22100.037.0316776
TAGGGAC14500.036.8689655
GCGTGTA301.3013431E-436.66666429
TTAGCGG4100.036.4878042
CCCTACA16450.036.376927
GGACTAA18600.036.3118258
TATGACG850.036.2352941
CGGTCTA850.036.23529431
Produced by FastQC (version 0.11.5)