##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546637_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1654904
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.030636822438037	31.0	31.0	33.0	30.0	34.0
2	31.406903361161735	31.0	31.0	34.0	30.0	34.0
3	31.57010497285643	31.0	31.0	34.0	30.0	34.0
4	35.35851868144618	37.0	35.0	37.0	33.0	37.0
5	35.18674315851554	37.0	35.0	37.0	32.0	37.0
6	35.244901214813666	37.0	35.0	37.0	32.0	37.0
7	35.68478473675815	37.0	35.0	37.0	35.0	37.0
8	35.71178932433543	37.0	35.0	37.0	35.0	37.0
9	37.48930632834291	39.0	37.0	39.0	35.0	39.0
10	36.769410793617034	39.0	37.0	39.0	32.0	39.0
11	36.42922852322552	38.0	35.0	39.0	32.0	39.0
12	35.99842830762389	37.0	35.0	39.0	31.0	39.0
13	35.904247617988716	38.0	35.0	39.0	31.0	39.0
14	36.868371216698975	39.0	35.0	41.0	31.0	41.0
15	36.99417186737116	39.0	35.0	41.0	31.0	41.0
16	37.01901560453054	39.0	35.0	41.0	31.0	41.0
17	36.89623869420824	38.0	35.0	40.0	31.0	41.0
18	36.83868671536234	38.0	35.0	40.0	31.0	41.0
19	36.80947051913585	38.0	35.0	40.0	31.0	41.0
20	36.68358950126412	38.0	35.0	40.0	31.0	41.0
21	36.586465438478605	38.0	35.0	40.0	31.0	41.0
22	36.50797629348893	38.0	35.0	40.0	30.0	41.0
23	36.2805461827393	38.0	35.0	40.0	30.0	41.0
24	36.21681378496879	38.0	35.0	40.0	30.0	41.0
25	36.073852018002256	38.0	34.0	40.0	30.0	41.0
26	35.97029978778225	38.0	34.0	40.0	30.0	41.0
27	35.81497174458458	38.0	34.0	40.0	29.0	41.0
28	35.76336089585861	38.0	34.0	40.0	29.0	41.0
29	35.63690038817962	38.0	34.0	40.0	29.0	41.0
30	35.62007343024127	38.0	34.0	40.0	29.0	41.0
31	35.55998353983071	38.0	34.0	40.0	29.0	41.0
32	35.38953981620686	38.0	34.0	40.0	27.0	41.0
33	35.2065588094536	38.0	34.0	40.0	27.0	41.0
34	35.10335644847073	38.0	34.0	40.0	26.0	41.0
35	34.879415361857845	38.0	33.0	40.0	25.0	41.0
36	34.69744166428989	37.0	33.0	40.0	24.0	41.0
37	34.77742938563204	37.0	33.0	40.0	25.0	41.0
38	34.721705911642005	37.0	33.0	40.0	24.0	41.0
39	34.64042566819586	37.0	33.0	40.0	24.0	41.0
40	34.642065038213694	37.0	33.0	40.0	24.0	41.0
41	34.65442285473961	37.0	33.0	40.0	24.0	41.0
42	34.407206702020176	37.0	33.0	40.0	23.0	41.0
43	34.46346495023276	37.0	33.0	40.0	24.0	41.0
44	34.26684569014275	37.0	33.0	40.0	23.0	41.0
45	34.07508048805248	36.0	33.0	40.0	23.0	41.0
46	34.126412166506334	36.0	33.0	40.0	23.0	41.0
47	33.97930997810145	36.0	33.0	40.0	23.0	41.0
48	34.03379410527741	36.0	33.0	40.0	23.0	41.0
49	33.94298581669994	36.0	33.0	40.0	23.0	41.0
50	33.46472544630988	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	3.0
14	13.0
15	42.0
16	127.0
17	317.0
18	819.0
19	1589.0
20	2969.0
21	4727.0
22	6902.0
23	10215.0
24	14491.0
25	20344.0
26	26799.0
27	32685.0
28	37319.0
29	42956.0
30	50999.0
31	61403.0
32	75260.0
33	93990.0
34	136987.0
35	171226.0
36	143544.0
37	174086.0
38	234496.0
39	310590.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.957616876870205	23.95274892078332	27.72221228542562	13.36742191692086
2	32.50756539352132	25.26448664091693	28.563711248507467	13.664236717054282
3	29.392158094971066	25.466552742636434	31.44617452130154	13.695114641090964
4	25.415431952548307	29.0386632698936	30.71120741746953	14.834697360088562
5	23.82198604873757	32.69404146705791	29.18574128771216	14.298231196492365
6	21.729477963676445	41.578846869667366	26.577251610969576	10.114423555686614
7	89.13737594446566	3.491139063051391	6.1120161652881375	1.25946882719481
8	89.3150901804576	3.0294204376809772	6.141564707076664	1.5139246747847608
9	83.70219662288568	5.50708681591198	7.907649023750018	2.8830675374523236
10	39.12148378395363	33.86383741896811	14.866662960510096	12.148015836568163
11	31.043009141315753	26.610304887775964	26.61090915243422	15.735776818474061
12	29.30907170446141	24.460693792510018	29.570718301484554	16.659516201544015
13	22.605601291676134	31.749152820949128	29.111718867076274	16.533527020298457
14	20.343476117043647	32.035392989563135	29.624618709000643	17.996512184392568
15	19.447472481787464	25.568733896346856	37.24820291690636	17.73559070495932
16	21.651165263966973	23.738234967103832	35.9380362848842	18.672563484044996
17	21.279663352073594	24.02804029720153	30.098664333399398	24.593632017325476
18	22.882717063950537	24.0200640037126	33.23932989466458	19.857889037672276
19	26.2989877358445	26.125140793665373	27.74378453372522	19.832086936764913
20	28.58655245258939	25.19251872011911	28.23438700976008	17.98654181753141
21	25.214332674282012	27.832309306159146	28.727043985633006	18.226314033925835
22	24.76838535649198	25.173303103986694	27.551567945935236	22.506743593586094
23	22.044360277091602	28.665408990491294	28.22206001073174	21.068170721685366
24	20.947378216500777	25.767234836582663	35.091521925138856	18.193865021777697
25	20.593581259094183	27.28430168759034	32.05472945862721	20.06738759468827
26	20.301842282090078	30.29003495066783	28.655136491301008	20.752986275941083
27	20.116030899677565	30.05805774836486	29.127490174656657	20.69842117730092
28	18.881397349936915	29.09769992700483	32.80516573771047	19.21573698534779
29	19.696127388658194	25.82530467024069	32.458680382668724	22.01988755843239
30	20.95311873075417	29.142052952920533	30.945964237200467	18.958864079124833
31	24.349267389528332	28.387568100626986	28.051415671241354	19.211748838603327
32	24.976735810657296	27.10622489280345	28.43028961196541	19.486749684573848
33	24.587528944277132	28.40956333418736	27.26762398302258	19.73528373851293
34	21.143703803966876	28.30430043071985	28.832065183237216	21.71993058207606
35	21.493814746958133	28.205865717890582	30.175889356724017	20.124430178427268
36	25.206295954327263	29.167311215635465	26.326421351329138	19.29997147870813
37	21.444446324378937	31.800696596298035	27.797080676583054	18.957776402739977
38	21.30721782048989	30.62612695358764	28.266110904318314	19.800544321604153
39	21.23609587021362	31.536572514176047	27.27898415859772	19.948347457012613
40	24.376217593286377	27.723239535344646	27.584077384549193	20.31646548681978
41	19.976808322416282	27.626436337092663	28.37892711601398	24.01782822447707
42	21.25361954530293	27.87829384665213	27.24037164693541	23.62771496110953
43	21.42813117860613	27.029604134137088	28.329558693434787	23.212705993822
44	20.470250842344935	28.794479921493938	29.04089904912913	21.694370187031996
45	20.136455045126482	31.624372169020077	26.602268167821215	21.636904618032222
46	21.726396213919358	30.433789512866007	27.606133044575394	20.233681228639245
47	20.429644257310393	29.40007396199417	28.682026268593226	21.488255512102214
48	21.34746184672948	27.46993179060538	30.420435263918634	20.762171098746514
49	21.10001546917525	28.135952296930817	29.97321899034627	20.790813243547664
50	20.58101255420254	30.471072642280156	28.906450162668047	20.041464640849256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1398.0
1	1986.5
2	2575.0
3	8666.5
4	14758.0
5	11045.0
6	7332.0
7	7597.5
8	7863.0
9	8141.5
10	8420.0
11	8688.5
12	8957.0
13	8792.5
14	8628.0
15	8542.0
16	8456.0
17	8324.5
18	8193.0
19	8155.0
20	8117.0
21	8957.0
22	9797.0
23	11274.5
24	12752.0
25	14355.5
26	15959.0
27	19841.5
28	23724.0
29	28988.5
30	34253.0
31	38749.0
32	43245.0
33	50135.0
34	57025.0
35	62061.0
36	67097.0
37	72882.0
38	78667.0
39	84637.0
40	90607.0
41	103160.0
42	115713.0
43	121010.5
44	126308.0
45	127910.5
46	129513.0
47	124737.0
48	119961.0
49	117221.0
50	114481.0
51	106992.5
52	99504.0
53	90441.5
54	81379.0
55	74007.5
56	66636.0
57	64347.5
58	62059.0
59	58328.0
60	54597.0
61	51385.5
62	48174.0
63	42511.0
64	36848.0
65	31666.5
66	26485.0
67	22175.0
68	17865.0
69	15290.0
70	12715.0
71	11030.5
72	9346.0
73	7960.0
74	6574.0
75	5305.0
76	4036.0
77	3361.0
78	2686.0
79	1967.0
80	1248.0
81	866.0
82	484.0
83	385.5
84	287.0
85	204.0
86	121.0
87	78.0
88	35.0
89	21.0
90	7.0
91	11.0
92	15.0
93	8.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1654904.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.688187322882726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29208645136475	17.309291486621266
2	5.818459479960368	2.640205972238873
3	2.478976633195527	1.6873045866896776
4	1.5746451801416677	1.429033792565143
5	1.2095048859845998	1.3720736710580255
6	0.9192076753796032	1.251309355658641
7	0.7571011344270382	1.202407665217336
8	0.6875102024391403	1.2478688207465793
9	0.6062655238624572	1.2379559195517353
>10	8.059497357992255	42.37360076945129
>50	1.376923360962486	21.258690995357394
>100	0.21820577523515686	6.258471130814325
>500	0.0010775593700494157	0.19411607858331667
>1k	2.693898425123539E-4	0.08238931798813315
>5k	2.693898425123539E-4	0.4552804374581631
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7449	0.4501167439319743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027735747813770466	0.0
2	0.0	0.0	0.0	0.08864562536558011	0.0
3	0.0	0.0	0.0	0.12683515176711158	0.0
4	0.0	0.0	0.0	0.1807355592831971	0.0
5	0.0	0.0	0.0	0.27856600745420884	0.0
6	0.0	0.0	0.0	0.46546506625157713	0.0
7	0.0	0.0	0.0	0.5580384118957957	0.0
8	0.0	0.0	0.0	0.7990795840725504	0.0
9	0.0	0.0	0.0	0.9215036038344219	0.0
10	0.0	0.0	0.0	1.1119678241154773	0.0
11	0.0	0.0	0.0	1.2932472215911013	0.0
12	0.0	0.0	0.0	1.4508394444632438	0.0
13	0.0	0.0	0.0	1.5433523636416373	0.0
14	0.0	0.0	0.0	1.5911496981093767	0.0
15	0.0	0.0	0.0	1.6373759444656608	0.0
16	0.0	0.0	0.0	1.7217312907576512	0.0
17	0.0	0.0	0.0	1.8151506069234227	0.0
18	0.0	0.0	0.0	1.9420461851563595	0.0
19	0.0	0.0	0.0	2.0032581950372954	0.0
20	0.0	0.0	0.0	2.071177542624829	0.0
21	0.0	0.0	0.0	2.152813697954685	0.0
22	0.0	0.0	0.0	2.2445410730773507	0.0
23	0.0	0.0	0.0	2.3546380938108795	0.0
24	0.0	0.0	0.0	2.441350072270053	0.0
25	0.0	0.0	0.0	2.518333389731368	0.0
26	0.0	0.0	0.0	2.592114104503947	0.0
27	0.0	0.0	0.0	2.6729647157780754	0.0
28	0.0	0.0	0.0	2.7479539598671585	0.0
29	0.0	0.0	0.0	2.8383519527416694	0.0
30	0.0	0.0	0.0	2.9580567815414067	0.0
31	0.0	0.0	0.0	3.0529867593528084	0.0
32	0.0	0.0	0.0	3.1660446769117727	0.0
33	0.0	0.0	0.0	3.265264933796764	0.0
34	0.0	0.0	0.0	3.3679899256996175	0.0
35	0.0	0.0	0.0	3.4841295930156675	0.0
36	0.0	0.0	0.0	3.5902988934705578	0.0
37	0.0	0.0	0.0	3.705955149060006	0.0
38	0.0	0.0	0.0	3.821550978183629	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATA	20	7.8574877E-4	44.0	24
TCGAACG	30	2.5283953E-6	44.0	1
CGTTTTT	6065	0.0	40.95301	1
CGCATCG	50	1.3478711E-9	39.6	21
CGTTAGG	170	0.0	38.823532	2
CGTAAGG	360	0.0	38.5	2
TAACGGG	415	0.0	38.168674	3
ACGGGAC	365	0.0	37.9726	5
TACGGGA	435	0.0	37.931034	4
AGGGATT	2870	0.0	37.790943	6
TCGTAGC	35	7.2921484E-6	37.714287	30
GGCGATA	955	0.0	37.089005	8
AGGGCGA	2210	0.0	37.031677	6
TAGGGAC	1450	0.0	36.868965	5
GCGTGTA	30	1.3013431E-4	36.666664	29
TTAGCGG	410	0.0	36.487804	2
CCCTACA	1645	0.0	36.3769	27
GGACTAA	1860	0.0	36.311825	8
TATGACG	85	0.0	36.235294	1
CGGTCTA	85	0.0	36.235294	31
>>END_MODULE