FastQCFastQC Report
Thu 26 May 2016
SRR1546636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546636_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3352481
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97430.2906205881554586No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40770.12161142747714306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG302.5291738E-644.00000411
ATTACGC254.4446726E-544.013
CGCGAAA207.8586437E-444.030
GTATCGC351.447188E-744.017
CGTTTTT102600.041.1910321
GGACGTA1200.040.3333368
GCGCGAC2300.040.1739169
CGACGGT1100.040.028
TTTCGCG557.8216544E-1140.01
TATCCGG2550.039.6862752
TACGCGC501.3496901E-939.614
TATTGCG3450.039.5362321
AGTAGCG950.039.368421
CGTAAGG8900.039.303372
GCGTAAG3150.039.111111
ACTACGT452.3532266E-839.11110728
TACGGGA14850.038.0740744
TATAGCG1450.037.9310341
ACGGGAC8950.037.854755
GGCGCGA2500.037.848
Produced by FastQC (version 0.11.5)