##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546636_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3352481
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97666951729182	31.0	31.0	33.0	30.0	34.0
2	31.344356015738793	31.0	31.0	34.0	30.0	34.0
3	31.510396628646067	31.0	31.0	34.0	30.0	34.0
4	35.274099987442135	37.0	35.0	37.0	33.0	37.0
5	35.070967740011056	37.0	35.0	37.0	32.0	37.0
6	35.15070540295381	37.0	35.0	37.0	32.0	37.0
7	35.70308526729905	37.0	35.0	37.0	35.0	37.0
8	35.750822748883586	37.0	35.0	37.0	35.0	37.0
9	37.52425532016438	39.0	37.0	39.0	35.0	39.0
10	36.753991148644836	39.0	37.0	39.0	32.0	39.0
11	36.32445821467743	38.0	35.0	39.0	32.0	39.0
12	35.850918469038305	37.0	35.0	39.0	31.0	39.0
13	35.70306498381348	37.0	35.0	39.0	30.0	39.0
14	36.644544741640594	38.0	35.0	40.0	31.0	41.0
15	36.849114133681894	38.0	35.0	40.0	31.0	41.0
16	36.90745033305185	38.0	35.0	40.0	31.0	41.0
17	36.79920721400062	38.0	35.0	40.0	31.0	41.0
18	36.74335484675379	38.0	35.0	40.0	31.0	41.0
19	36.740237453993025	38.0	35.0	40.0	31.0	41.0
20	36.61035841813869	38.0	35.0	40.0	31.0	41.0
21	36.51351133682786	38.0	35.0	40.0	31.0	41.0
22	36.43472878742639	38.0	35.0	40.0	30.0	41.0
23	36.238392402522194	38.0	34.0	40.0	30.0	41.0
24	36.21969967913316	38.0	34.0	40.0	30.0	41.0
25	36.097114942635024	38.0	34.0	40.0	30.0	41.0
26	35.989967131804775	38.0	34.0	40.0	30.0	41.0
27	35.808348801976805	38.0	34.0	40.0	29.0	41.0
28	35.80247166203179	38.0	34.0	40.0	29.0	41.0
29	35.71285862619356	38.0	34.0	40.0	29.0	41.0
30	35.67415266484732	38.0	34.0	40.0	29.0	41.0
31	35.620523427276694	38.0	34.0	40.0	29.0	41.0
32	35.444656957041666	38.0	34.0	40.0	28.0	41.0
33	35.25267376608547	38.0	34.0	40.0	27.0	41.0
34	35.185270252090916	38.0	34.0	40.0	27.0	41.0
35	34.97139610932918	38.0	34.0	40.0	25.0	41.0
36	34.78450675783099	37.0	33.0	40.0	25.0	41.0
37	34.856453176020985	38.0	33.0	40.0	25.0	41.0
38	34.83457654196996	38.0	33.0	40.0	25.0	41.0
39	34.73778822310999	37.0	33.0	40.0	24.0	41.0
40	34.745287445327804	37.0	33.0	40.0	24.0	41.0
41	34.77731656048162	37.0	34.0	40.0	24.0	41.0
42	34.54814121243342	37.0	33.0	40.0	24.0	41.0
43	34.62411449908292	37.0	33.0	40.0	24.0	41.0
44	34.45057824339646	37.0	33.0	40.0	24.0	41.0
45	34.26934679122716	37.0	33.0	40.0	23.0	41.0
46	34.34651769838516	37.0	33.0	40.0	23.0	41.0
47	34.220584098761485	37.0	33.0	40.0	23.0	41.0
48	34.28409348181243	37.0	33.0	40.0	23.0	41.0
49	34.22776952352601	37.0	33.0	40.0	24.0	41.0
50	33.72045866926614	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	7.0
14	13.0
15	71.0
16	272.0
17	738.0
18	1618.0
19	3281.0
20	5864.0
21	9411.0
22	14137.0
23	20524.0
24	29088.0
25	40575.0
26	52536.0
27	63710.0
28	73973.0
29	86364.0
30	104041.0
31	125860.0
32	154130.0
33	192196.0
34	278778.0
35	359448.0
36	275529.0
37	337041.0
38	466321.0
39	656952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.53673920896196	24.540631251899715	29.879751742068038	13.04287779707029
2	29.064892537795146	26.774618558613756	31.185680097814128	12.974808805776975
3	27.981247321013903	26.542163848206744	32.36880387987285	13.107784950906506
4	23.67685305300761	29.582002105306486	32.22228552525726	14.518859316428639
5	22.4313575528094	33.897582119033636	30.27229684523193	13.398763482925032
6	20.826158298883723	43.16794039996051	25.775985009311015	10.229916291844757
7	90.32585121287786	3.359273326232125	5.234750025428929	1.080125435461081
8	90.79598064836162	2.7034008544716586	4.812853525493507	1.6877649716732175
9	85.09787229219197	5.89399313523328	6.6124162970647715	2.3957182755099877
10	39.18930487600079	39.565086274911025	11.78300488503887	9.462603964049311
11	29.3053114991554	25.558563941152833	30.430657175983995	14.705467383707768
12	29.90632907390079	24.745643599471556	28.57969963140731	16.768327695220346
13	20.94332525672778	33.76997513185011	29.54975732897517	15.73694228244694
14	17.07025334371768	35.5670322963799	29.859408599183705	17.503305760718703
15	15.987801273146662	27.30610553795831	40.03309787587163	16.6729953130234
16	18.614512654956137	24.473934378748158	39.35255710621477	17.558995860080937
17	18.757391913630535	24.38901219723542	31.050854576058747	25.802741313075302
18	19.835339857257953	25.794061174395917	35.179200120746394	19.191398847599732
19	24.398497709606705	26.47677943588644	29.357571303163237	19.767151551343616
20	25.79620883757432	25.89577688881757	29.82131740642229	18.486696867185827
21	21.19624242464014	29.641122500023116	30.672239454899223	18.49039562043752
22	21.97011705659182	26.010766354827965	29.115094164590342	22.904022423989876
23	18.69877860605325	30.685095605314395	29.10089572468867	21.515230063943687
24	18.12272761575681	26.727131339446814	37.35054725142365	17.79959379337273
25	17.660920375089372	28.22178559699518	34.51020304067346	19.607090987241985
26	17.968931069258858	33.02995602361356	29.326072243213307	19.675040663914277
27	17.49038995299302	33.24170964727317	29.973861149399504	19.294039250334304
28	16.740109787348533	30.440739261460394	35.15778314627286	17.661367804918207
29	17.143930122199052	26.479613158135724	34.82137557230004	21.55508114736519
30	18.37749416029502	31.104397012242575	31.478269377216456	19.039839450245953
31	23.24818544832916	29.221701778473914	28.707485590522364	18.822627182674562
32	24.509788422365407	27.18550828475985	29.79298018392945	18.511723108945286
33	23.48949926934709	28.846516952668782	28.313150768043126	19.350833009940995
34	19.79536945921543	28.22634938124929	29.517423066678084	22.4608580928572
35	18.85767585259991	27.777547434273302	32.558752756540606	20.80602395658618
36	25.009865827725797	27.844542593977415	28.114014665556642	19.031576912740146
37	19.430624662749764	31.718330394713647	30.644140861648435	18.206904080888155
38	19.462541323873275	31.796451642828107	28.630945261136453	20.11006177216217
39	19.638381246605128	31.492139701910315	30.035755609054902	18.83372344242965
40	23.086394822222704	27.391982236439222	29.490189504429704	20.031433436908365
41	17.72391849498923	27.536114298634356	30.39790531251333	24.342061893863082
42	19.834325682979262	27.607136326797978	29.000462642443015	23.55807534777975
43	19.89195464493311	27.847257001605673	29.756589224517604	22.504199128943608
44	19.288192833904205	29.764911419333924	30.52449812541816	20.422397621343716
45	18.025337056347222	32.95845673696584	28.002246694313854	21.013959512373077
46	20.248257931961433	31.806712700236034	28.3589377538605	19.586091613942035
47	19.785824289533632	28.533823159624173	29.499675016800992	22.18067753404121
48	20.231852171570846	27.237887403388715	32.36265917689019	20.16760124815025
49	20.65780536862103	26.607518431871796	32.05810860673036	20.676567592776813
50	19.55727713296511	31.130914686764815	29.8997369410893	19.412071239180772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2744.0
1	3633.5
2	4523.0
3	12448.5
4	20374.0
5	16485.0
6	12596.0
7	13302.5
8	14009.0
9	15092.5
10	16176.0
11	16732.0
12	17288.0
13	17551.5
14	17815.0
15	18295.5
16	18776.0
17	18534.0
18	18292.0
19	19023.5
20	19755.0
21	20611.0
22	21467.0
23	24796.0
24	28125.0
25	32476.0
26	36827.0
27	47421.5
28	58016.0
29	69120.0
30	80224.0
31	91719.5
32	103215.0
33	119115.0
34	135015.0
35	149072.5
36	163130.0
37	168981.5
38	174833.0
39	192672.5
40	210512.0
41	245795.0
42	281078.0
43	296534.5
44	311991.0
45	314435.5
46	316880.0
47	302982.0
48	289084.0
49	268025.0
50	246966.0
51	218770.0
52	190574.0
53	167328.0
54	144082.0
55	124534.0
56	104986.0
57	94206.5
58	83427.0
59	78216.5
60	73006.0
61	61684.5
62	50363.0
63	42715.5
64	35068.0
65	27689.5
66	20311.0
67	16139.0
68	11967.0
69	9329.5
70	6692.0
71	5615.0
72	4538.0
73	3917.0
74	3296.0
75	2827.5
76	2359.0
77	1662.5
78	966.0
79	817.0
80	668.0
81	419.5
82	171.0
83	180.0
84	189.0
85	131.5
86	74.0
87	43.0
88	12.0
89	9.0
90	6.0
91	9.0
92	12.0
93	6.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3352481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.305843972134788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49740193006743	15.540702818241034
2	7.214700660434277	2.7857177051200397
3	2.373309698266678	1.3745624019687237
4	1.196691468981358	0.9241255513175551
5	0.7493204850919098	0.7233132185154382
6	0.5582537726017777	0.6466536138423321
7	0.4232641900211228	0.5720030688077865
8	0.33710157660173784	0.5206424352507035
9	0.3220365620653548	0.5595468858500796
>10	3.803553963898813	17.537278421254523
>50	1.331245875235024	18.513084883200506
>100	1.1859296541476256	38.89155465624318
>500	0.005783391645978465	0.6869994711087527
>1k	0.0012504630585899383	0.42980468536422584
>5k	1.5630788232374229E-4	0.294010183915162
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9743	0.2906205881554586	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4077	0.12161142747714306	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.056018214570045285	0.0
2	0.0	0.0	0.0	0.1919473965698836	0.0
3	0.0	0.0	0.0	0.26282028145722525	0.0
4	0.0	0.0	0.0	0.4215982133828648	0.0
5	0.0	0.0	0.0	0.6476695915651722	0.0
6	0.0	0.0	0.0	1.00904374998695	0.0
7	0.0	0.0	0.0	1.184376585579456	0.0
8	0.0	0.0	0.0	1.6939096746558742	0.0
9	0.0	0.0	0.0	1.9175052744519656	0.0
10	0.0	0.0	0.0	2.2884842598660513	0.0
11	0.0	0.0	0.0	2.583907261517664	0.0
12	0.0	0.0	0.0	2.8449974809700636	0.0
13	0.0	0.0	0.0	2.969293487420212	0.0
14	0.0	0.0	0.0	3.0276979944107065	0.0
15	0.0	0.0	0.0	3.091322516070934	0.0
16	0.0	0.0	0.0	3.21794515763102	0.0
17	0.0	0.0	0.0	3.3631808800706104	0.0
18	0.0	0.0	0.0	3.5656279632904706	0.0
19	0.0	0.0	0.0	3.6640923542892563	0.0
20	0.0	0.0	0.0	3.7718036284172825	0.0
21	0.0	0.0	0.0	3.9058237764807617	0.0
22	0.0	0.0	0.0	4.060216896083825	0.0
23	0.0	0.0	0.0	4.232477380185003	0.0
24	0.0	0.0	0.0	4.363991921207011	0.0
25	0.0	0.0	0.0	4.475998521691846	0.0
26	0.0	0.0	0.0	4.592479420465023	0.0
27	0.0	0.0	0.0	4.706126596988916	0.0
28	0.0	0.0	0.0	4.8281556256396385	0.0
29	0.0	0.0	0.0	4.958834964314488	0.0
30	0.0	0.0	0.0	5.134108142596483	0.0
31	0.0	0.0	0.0	5.284951652224129	0.0
32	0.0	0.0	0.0	5.428636284590427	0.0
33	0.0	0.0	0.0	5.572917490061838	0.0
34	0.0	0.0	0.0	5.715408976217912	0.0
35	0.0	0.0	0.0	5.891308556260274	0.0
36	0.0	0.0	0.0	6.046596535521006	0.0
37	0.0	0.0	0.0	6.210504996150612	0.0
38	0.0	0.0	0.0	6.377634951547824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	30	2.5291738E-6	44.000004	11
ATTACGC	25	4.4446726E-5	44.0	13
CGCGAAA	20	7.8586437E-4	44.0	30
GTATCGC	35	1.447188E-7	44.0	17
CGTTTTT	10260	0.0	41.191032	1
GGACGTA	120	0.0	40.333336	8
GCGCGAC	230	0.0	40.173916	9
CGACGGT	110	0.0	40.0	28
TTTCGCG	55	7.8216544E-11	40.0	1
TATCCGG	255	0.0	39.686275	2
TACGCGC	50	1.3496901E-9	39.6	14
TATTGCG	345	0.0	39.536232	1
AGTAGCG	95	0.0	39.36842	1
CGTAAGG	890	0.0	39.30337	2
GCGTAAG	315	0.0	39.11111	1
ACTACGT	45	2.3532266E-8	39.111107	28
TACGGGA	1485	0.0	38.074074	4
TATAGCG	145	0.0	37.931034	1
ACGGGAC	895	0.0	37.85475	5
GGCGCGA	250	0.0	37.84	8
>>END_MODULE