FastQCFastQC Report
Thu 26 May 2016
SRR1546635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546635_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2737084
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84290.3079554737815865No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33940.12400057871808098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG302.5290046E-644.01
TAACGCG254.4444565E-544.01
GTAATCG254.4444565E-544.02
CGTAATC207.85839E-444.024
TCGCGAA207.85839E-444.016
TACGGGA9950.041.125634
CGTTAGG4750.040.7578962
CGTTTTT77600.040.3144341
TCATCGA3550.039.66197216
ACGCGAA452.3530447E-839.1111114
GCGATCG900.039.111119
CGTACAC452.3530447E-839.1111115
TAATGCG900.039.111111
TATTGCG2500.038.721
ACGGGTA1650.038.6666685
AGGGCGA29750.038.305886
TAGGGAT61350.037.724535
GTGCGAC357.2938965E-637.7142879
TACGGGT1050.037.7142834
ATACCGG1050.037.7142832
Produced by FastQC (version 0.11.5)