##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546635_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2737084
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96165298543998	31.0	31.0	33.0	30.0	34.0
2	31.33273074556718	31.0	31.0	34.0	30.0	34.0
3	31.504709391454554	31.0	31.0	34.0	30.0	34.0
4	35.270570066537964	37.0	35.0	37.0	33.0	37.0
5	35.06659934441179	37.0	35.0	37.0	32.0	37.0
6	35.145644781088194	37.0	35.0	37.0	32.0	37.0
7	35.674203641539684	37.0	35.0	37.0	35.0	37.0
8	35.71873753235195	37.0	35.0	37.0	35.0	37.0
9	37.49658614788585	39.0	37.0	39.0	35.0	39.0
10	36.73863754272796	39.0	37.0	39.0	32.0	39.0
11	36.3238468384602	38.0	35.0	39.0	32.0	39.0
12	35.82801514312312	37.0	35.0	39.0	31.0	39.0
13	35.66895864357835	37.0	35.0	39.0	30.0	39.0
14	36.60843803113094	38.0	35.0	40.0	31.0	41.0
15	36.82575982322793	38.0	35.0	40.0	31.0	41.0
16	36.88775645906373	38.0	35.0	40.0	31.0	41.0
17	36.78929546919276	38.0	35.0	40.0	31.0	41.0
18	36.72144735053802	38.0	35.0	40.0	31.0	41.0
19	36.70609889941266	38.0	35.0	40.0	31.0	41.0
20	36.577754646916205	38.0	35.0	40.0	31.0	41.0
21	36.46556992770408	38.0	35.0	40.0	30.0	41.0
22	36.39661844503128	38.0	35.0	40.0	30.0	41.0
23	36.19453038342996	38.0	34.0	40.0	30.0	41.0
24	36.17018147780631	38.0	34.0	40.0	30.0	41.0
25	36.03379107108149	38.0	34.0	40.0	30.0	41.0
26	35.91175353039951	38.0	34.0	40.0	30.0	41.0
27	35.75062219500753	38.0	34.0	40.0	29.0	41.0
28	35.718151141872156	38.0	34.0	40.0	29.0	41.0
29	35.64419104419156	38.0	34.0	40.0	29.0	41.0
30	35.60904305457925	38.0	34.0	40.0	29.0	41.0
31	35.54946176295649	38.0	34.0	40.0	29.0	41.0
32	35.36720246802802	38.0	34.0	40.0	27.0	41.0
33	35.19275586719297	38.0	34.0	40.0	27.0	41.0
34	35.12969715215171	38.0	34.0	40.0	26.0	41.0
35	34.92679727768677	37.0	34.0	40.0	25.0	41.0
36	34.72644281286215	37.0	33.0	40.0	24.0	41.0
37	34.80493072189235	37.0	33.0	40.0	25.0	41.0
38	34.78806386650903	37.0	33.0	40.0	25.0	41.0
39	34.68543128380422	37.0	33.0	40.0	24.0	41.0
40	34.65801049584156	37.0	33.0	40.0	24.0	41.0
41	34.676018711884616	37.0	33.0	40.0	24.0	41.0
42	34.43509479431395	37.0	33.0	40.0	23.0	41.0
43	34.504047738396046	37.0	33.0	40.0	24.0	41.0
44	34.329162714772366	37.0	33.0	40.0	23.0	41.0
45	34.162878815556994	37.0	33.0	40.0	23.0	41.0
46	34.2383825268059	37.0	33.0	40.0	23.0	41.0
47	34.099708668056955	37.0	33.0	40.0	23.0	41.0
48	34.16151897420759	36.0	33.0	40.0	23.0	41.0
49	34.06863435685569	36.0	33.0	40.0	23.0	41.0
50	33.57335763169855	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	5.0
14	27.0
15	80.0
16	221.0
17	647.0
18	1550.0
19	2947.0
20	5140.0
21	8028.0
22	12023.0
23	17452.0
24	24701.0
25	33784.0
26	44202.0
27	53370.0
28	61679.0
29	71827.0
30	86274.0
31	104452.0
32	126092.0
33	156373.0
34	227655.0
35	295385.0
36	223787.0
37	274023.0
38	377531.0
39	527824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.17377179509288	24.803257773601395	29.278275712400497	12.744694718905228
2	28.699046138152866	27.137493770742875	31.12750649961784	13.035953591486413
3	28.16935833902065	26.106140695718512	32.2624734936889	13.462027471571936
4	24.041461643120925	29.52233106473897	31.928906822004734	14.50730047013537
5	22.037358005819332	34.33223094358814	30.233379757435287	13.397031293157244
6	20.813573861817904	43.20338725446497	25.873484335884466	10.109554547832657
7	90.65647966960458	3.281484967213282	5.0607873196438256	1.0012480435383058
8	91.01594982105044	2.6132921021057447	4.762513682444529	1.6082443943992952
9	85.19986964229085	5.974021988364259	6.587156258266097	2.238952111078798
10	40.425942353248935	37.76829647902659	11.582107089150352	10.223654078574132
11	30.10196252654285	25.398818596725565	29.82392940808539	14.675289468646193
12	30.46936082341645	23.74282265359777	29.333663124697672	16.454153398288103
13	21.633351406094953	32.938557968991816	29.312253478519473	16.11583714639375
14	17.564057222942374	34.9022536392745	29.916655827881062	17.617033309902073
15	16.263841372789436	26.380337614775435	40.33106035474249	17.02476065769264
16	19.18041243893136	23.796164092881327	39.19054000534876	17.832883462838552
17	19.229186974166666	23.552218346240014	30.844394983858734	26.374199695734585
18	20.35173198922649	25.39231532536086	34.42199800956054	19.833954675852112
19	24.821488854562006	26.00632644083996	29.318610608954636	19.853574095643392
20	26.381506742211787	26.06686532090356	28.9713797603581	18.580248176526553
21	22.846174980380578	28.990158869804507	29.850015564009	18.313650585805917
22	22.68282595638278	26.224514848649143	28.256166051169785	22.83649314379829
23	19.308870316000533	30.011135938831252	28.695027262590404	21.984966482577807
24	18.94607545840756	26.140483814161346	36.57235218210329	18.341088545327803
25	18.470130986115148	27.908058356995983	33.80784075315189	19.813969903736968
26	18.38577113453588	32.06237002591079	29.5916749358076	19.960183903745737
27	18.292094798698177	32.83490751471273	29.640997499528694	19.23200018706039
28	17.062136200423517	30.439000045303686	34.5961614623446	17.9027022919282
29	17.538628701201716	26.356772389886462	34.25700490010537	21.847594008806453
30	19.11106856786273	30.238056267180692	31.541523753015984	19.109351411940594
31	24.174376818541194	28.321892934232196	28.75965808868124	18.74407215854537
32	24.6521480524529	27.32963255786085	29.803542748414007	18.214676641272245
33	24.325924962478314	27.97447940947373	28.07436673481705	19.625228893230897
34	20.300728804815634	28.24549776331307	29.519079429056617	21.934694002814677
35	20.07212785577644	27.202745695784277	31.60125885796709	21.123867590472194
36	25.184868275873157	26.50327867175432	28.764188457497102	19.54766459487542
37	19.471671311512544	31.348910007876995	30.687256949366553	18.492161731243907
38	20.39824134005387	30.499210108275815	28.652134899769244	20.450413651901076
39	20.475074933761626	31.03675298237102	29.004699892294134	19.483472191573224
40	23.080329284742447	27.273258694289247	28.680741986727483	20.96567003424082
41	19.043551458413408	26.745543797705878	30.44100948308492	23.769895260795796
42	20.505654923268704	27.07600497463724	28.760900286582363	23.65743981551169
43	20.418518394028098	26.779448493360086	29.96579571544023	22.836237397171587
44	19.244641377465946	29.088840532479093	30.727774522082623	20.938743567972338
45	18.528806569327063	32.395352133876784	28.176848061659783	20.89899323513637
46	20.695966948767374	30.937888643534507	28.698680785829005	19.667463621869114
47	19.93607065037098	28.422730175617556	29.835511076751754	21.80568809725971
48	20.5711625949368	27.128213821716834	31.83022515933015	20.470398424016214
49	20.747189344572543	26.221592030058265	32.42487260164467	20.606346023724516
50	19.986050848275024	30.15227154153837	30.00711706326879	19.854560546917817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1906.0
1	2898.5
2	3891.0
3	10548.0
4	17205.0
5	13479.0
6	9753.0
7	10533.5
8	11314.0
9	12178.0
10	13042.0
11	13407.0
12	13772.0
13	13806.5
14	13841.0
15	13963.5
16	14086.0
17	13893.5
18	13701.0
19	14054.0
20	14407.0
21	15776.5
22	17146.0
23	19603.5
24	22061.0
25	25612.5
26	29164.0
27	38047.5
28	46931.0
29	55462.5
30	63994.0
31	75182.5
32	86371.0
33	95851.5
34	105332.0
35	113374.5
36	121417.0
37	128530.0
38	135643.0
39	154255.5
40	172868.0
41	194099.0
42	215330.0
43	228837.0
44	242344.0
45	247679.0
46	253014.0
47	243564.0
48	234114.0
49	215146.5
50	196179.0
51	175052.0
52	153925.0
53	136127.0
54	118329.0
55	104183.5
56	90038.0
57	83671.5
58	77305.0
59	71137.0
60	64969.0
61	60112.5
62	55256.0
63	46934.5
64	38613.0
65	32017.0
66	25421.0
67	20976.0
68	16531.0
69	13786.5
70	11042.0
71	8967.0
72	6892.0
73	5763.0
74	4634.0
75	3669.5
76	2705.0
77	2071.0
78	1437.0
79	1051.0
80	665.0
81	471.5
82	278.0
83	193.0
84	108.0
85	87.5
86	67.0
87	47.0
88	27.0
89	17.5
90	8.0
91	7.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2737084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.642776619538047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3033599766717	15.773809618201144
2	6.825561382448478	2.6814595507676153
3	2.273793272857412	1.3399084001323944
4	1.1888857545937344	0.9341206921454261
5	0.7842861310727829	0.7702778639232206
6	0.5952077373643972	0.7014919576361716
7	0.45732259477471976	0.6288159900559137
8	0.37843894035399395	0.5946873255606548
9	0.3186106632603777	0.5632558278323812
>10	4.29085452371111	20.291652576510298
>50	1.5220021550376612	21.47887193716201
>100	1.0564005256845481	33.17557913607153
>500	0.004711019793888772	0.5799510792976099
>1k	3.7688158351110177E-4	0.17411857780290765
>5k	1.8844079175555088E-4	0.3119994669006608
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8429	0.3079554737815865	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3394	0.12400057871808098	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03182218740820523	0.0
2	0.0	0.0	0.0	0.10956916192561135	0.0
3	0.0	0.0	0.0	0.16305674213871404	0.0
4	0.0	0.0	0.0	0.28727653225111105	0.0
5	0.0	0.0	0.0	0.4572384333107789	0.0
6	0.0	0.0	0.0	0.7644997376770315	0.0
7	0.0	0.0	0.0	0.9149518246425759	0.0
8	0.0	0.0	0.0	1.3711307362141607	0.0
9	0.0	0.0	0.0	1.5916573988960514	0.0
10	0.0	0.0	0.0	1.9491911830254387	0.0
11	0.0	0.0	0.0	2.2393905338674296	0.0
12	0.0	0.0	0.0	2.474750500898036	0.0
13	0.0	0.0	0.0	2.605327421445597	0.0
14	0.0	0.0	0.0	2.6673642460370233	0.0
15	0.0	0.0	0.0	2.7284876898187997	0.0
16	0.0	0.0	0.0	2.8407239237085893	0.0
17	0.0	0.0	0.0	2.96709929253176	0.0
18	0.0	0.0	0.0	3.1517483570105997	0.0
19	0.0	0.0	0.0	3.2451324109892132	0.0
20	0.0	0.0	0.0	3.3454581591211667	0.0
21	0.0	0.0	0.0	3.459740366024572	0.0
22	0.0	0.0	0.0	3.5861888053125153	0.0
23	0.0	0.0	0.0	3.7348141306587594	0.0
24	0.0	0.0	0.0	3.849425154653639	0.0
25	3.653523238599912E-5	0.0	0.0	3.9491663390674163	0.0
26	3.653523238599912E-5	0.0	0.0	4.043244562461364	0.0
27	3.653523238599912E-5	0.0	0.0	4.142291577459807	0.0
28	3.653523238599912E-5	0.0	0.0	4.242836536986077	0.0
29	3.653523238599912E-5	0.0	0.0	4.352771051235549	0.0
30	3.653523238599912E-5	0.0	0.0	4.503113532503935	0.0
31	3.653523238599912E-5	0.0	0.0	4.632229043756055	0.0
32	3.653523238599912E-5	0.0	0.0	4.756120016776979	0.0
33	3.653523238599912E-5	0.0	0.0	4.876759354115547	0.0
34	3.653523238599912E-5	0.0	0.0	5.005034555022791	0.0
35	7.307046477199823E-5	0.0	0.0	5.158701742438303	0.0
36	7.307046477199823E-5	0.0	0.0	5.299545063286329	0.0
37	7.307046477199823E-5	0.0	0.0	5.4439688369081844	0.0
38	7.307046477199823E-5	0.0	0.0	5.590511654008426	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCG	30	2.5290046E-6	44.0	1
TAACGCG	25	4.4444565E-5	44.0	1
GTAATCG	25	4.4444565E-5	44.0	2
CGTAATC	20	7.85839E-4	44.0	24
TCGCGAA	20	7.85839E-4	44.0	16
TACGGGA	995	0.0	41.12563	4
CGTTAGG	475	0.0	40.757896	2
CGTTTTT	7760	0.0	40.314434	1
TCATCGA	355	0.0	39.661972	16
ACGCGAA	45	2.3530447E-8	39.11111	14
GCGATCG	90	0.0	39.11111	9
CGTACAC	45	2.3530447E-8	39.11111	15
TAATGCG	90	0.0	39.11111	1
TATTGCG	250	0.0	38.72	1
ACGGGTA	165	0.0	38.666668	5
AGGGCGA	2975	0.0	38.30588	6
TAGGGAT	6135	0.0	37.72453	5
GTGCGAC	35	7.2938965E-6	37.714287	9
TACGGGT	105	0.0	37.714283	4
ATACCGG	105	0.0	37.714283	2
>>END_MODULE