FastQCFastQC Report
Thu 26 May 2016
SRR1546634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546634_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4328373
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218780.5054555141158121No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG254.4448872E-544.025
CGTTTTT209450.042.382431
GCGCGAC2700.039.9259269
CGCGTAG404.128069E-738.51
TACGGGA22700.038.3788534
CGCGACC2850.037.8245610
CGTCGGA357.294877E-637.71428742
AGGGCGA46400.037.599146
ACGGGAT28350.037.4814835
TATAGCG2950.037.288141
ACGTCGG651.0913936E-1137.2307662
CGTAAGG9850.037.076142
AGGGACC51650.036.8441436
AGGGATT156050.036.6831176
ACGTCGA301.3016904E-436.66666431
AATCGCG601.9826984E-1036.6666641
CGAGGGA37700.036.6472134
ATAGGGA88400.036.6334884
TAGGGAT119500.036.5623445
TAGGGCG16900.036.3195275
Produced by FastQC (version 0.11.5)