FastQCFastQC Report
Thu 26 May 2016
SRR1546633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546633_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2308644
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79140.34279862984505194No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26460.11461273370861856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65800.041.124621
TACGGGA6200.040.8064544
TAATGCG650.040.6153831
TATCACG501.3496901E-939.6000021
ATTAGCG1500.038.133331
AGGGTAC5450.037.9449546
GTTACGG2150.037.8604662
TGTACGG3900.037.7948722
CGTAAGG5450.037.5412832
GCGATAA1700.037.529419
CTAGACG651.0913936E-1137.2307661
TTAGGGA26850.036.7076344
AGGGCGA25600.036.6953126
TCGTAAG2100.036.6666681
TTACGCG900.036.6666681
TACGGTC601.9826984E-1036.6666644
ACGGGAT11000.036.45
TCGTCCC7800.036.38461738
ACCGGGT850.036.2352944
TAGCACG850.036.2352941
Produced by FastQC (version 0.11.5)