##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546633_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2308644
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.902467855589688	31.0	31.0	33.0	30.0	34.0
2	31.258940313014914	31.0	31.0	34.0	30.0	34.0
3	31.429805981346625	31.0	31.0	34.0	30.0	34.0
4	35.20816332011345	37.0	35.0	37.0	33.0	37.0
5	35.00210773077183	35.0	35.0	37.0	32.0	37.0
6	35.07374891927902	37.0	35.0	37.0	32.0	37.0
7	35.62833334199643	37.0	35.0	37.0	35.0	37.0
8	35.66474432610658	37.0	35.0	37.0	35.0	37.0
9	37.40906610113989	39.0	37.0	39.0	35.0	39.0
10	36.62035766449916	39.0	35.0	39.0	32.0	39.0
11	36.2338346665835	38.0	35.0	39.0	31.0	39.0
12	35.733462586695914	37.0	35.0	39.0	30.0	39.0
13	35.57738048828663	37.0	35.0	39.0	30.0	39.0
14	36.4822761759717	38.0	35.0	40.0	30.0	41.0
15	36.66996643917382	38.0	35.0	40.0	31.0	41.0
16	36.73004412980087	38.0	35.0	40.0	31.0	41.0
17	36.632672685784385	38.0	35.0	40.0	31.0	41.0
18	36.56068887190922	38.0	35.0	40.0	31.0	41.0
19	36.561954116788904	38.0	35.0	40.0	31.0	41.0
20	36.43484400366622	38.0	35.0	40.0	30.0	41.0
21	36.31551984628206	38.0	34.0	40.0	30.0	41.0
22	36.24532842655689	38.0	34.0	40.0	30.0	41.0
23	36.03721621869808	38.0	34.0	40.0	30.0	41.0
24	35.99822839727563	38.0	34.0	40.0	30.0	41.0
25	35.821012681037004	38.0	34.0	40.0	29.0	41.0
26	35.714779758161065	38.0	34.0	40.0	29.0	41.0
27	35.50696599389079	37.0	34.0	40.0	28.0	41.0
28	35.46174984103222	37.0	34.0	40.0	28.0	41.0
29	35.32755808171377	37.0	34.0	40.0	27.0	41.0
30	35.329180679221224	37.0	34.0	40.0	27.0	41.0
31	35.29118218313434	37.0	34.0	40.0	27.0	41.0
32	35.1229968760883	37.0	34.0	40.0	27.0	41.0
33	34.96284095772237	37.0	33.0	40.0	26.0	41.0
34	34.864893417954434	37.0	34.0	40.0	25.0	41.0
35	34.719236486872816	37.0	33.0	40.0	25.0	41.0
36	34.52868567002968	37.0	33.0	40.0	24.0	41.0
37	34.62710578157568	37.0	33.0	40.0	24.0	41.0
38	34.57557250056743	37.0	33.0	40.0	24.0	41.0
39	34.48224845407088	37.0	33.0	40.0	24.0	41.0
40	34.45659183486064	37.0	33.0	40.0	24.0	41.0
41	34.46369297301793	37.0	33.0	40.0	24.0	41.0
42	34.20231529850423	37.0	33.0	40.0	23.0	41.0
43	34.256692240120174	37.0	33.0	40.0	23.0	41.0
44	34.060870796883364	36.0	33.0	40.0	23.0	41.0
45	33.878266636172576	36.0	33.0	40.0	23.0	41.0
46	33.94691082730815	36.0	33.0	40.0	23.0	41.0
47	33.79198915034107	36.0	32.0	40.0	23.0	41.0
48	33.844933216208304	36.0	33.0	40.0	23.0	41.0
49	33.76113510788151	36.0	32.0	40.0	23.0	41.0
50	33.25710070500259	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	6.0
14	17.0
15	68.0
16	207.0
17	597.0
18	1355.0
19	2660.0
20	4610.0
21	7421.0
22	10851.0
23	16096.0
24	22457.0
25	30069.0
26	39385.0
27	47857.0
28	55320.0
29	65300.0
30	77424.0
31	92972.0
32	112242.0
33	138187.0
34	195876.0
35	244014.0
36	195048.0
37	234475.0
38	308467.0
39	405658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.564142414334995	23.668439135700435	28.640102155204527	13.12731629476004
2	30.28574349271694	26.00604510699787	30.199978862050624	13.508232538234566
3	28.9707724534402	26.344512189839577	31.086559902696127	13.598155454024093
4	24.645246300425704	29.479209440693328	30.615980636252278	15.259563622628693
5	22.296811461619896	33.24804517283739	29.649525868865013	14.805617496677703
6	21.530257588437195	41.43124708703464	26.69194557497821	10.346549749549952
7	88.88100547334278	3.6896550529228413	5.9108290407702535	1.5185104329641124
8	88.92384447320592	3.09701279192461	5.569416505966273	2.4097262289032004
9	82.88228934387459	6.003524146641925	8.116366143935574	2.997820365547915
10	40.356936799263984	33.968511385904456	13.890491561280127	11.784060253551436
11	32.04859649213997	25.65293739528485	27.455467365258567	14.842998747316608
12	30.803839829787528	23.479930210114684	28.916801377778473	16.79942858231932
13	22.669757658608255	31.236864583712343	29.42952659656491	16.66385116111449
14	18.868521954879142	33.505382380306365	29.666202324827907	17.95989333998659
15	17.38401416589132	26.548701315577457	39.00467114028841	17.062613378242812
16	19.761816893379837	23.72254015777227	37.967135686576185	18.548507262271706
17	20.375337210934212	24.301754623060116	30.152938261594254	25.16996990441142
18	21.529261332626426	24.925540707012427	32.95341334566958	20.59178461469157
19	25.91703181607905	26.885955565258218	27.50233470383481	19.694677914827924
20	27.52109030235931	26.362141586143206	27.36978936553232	18.746978745965166
21	23.445840935198326	29.455342616704872	28.95808102072039	18.140735427376416
22	23.053792615925193	25.596757230651413	27.98694818256951	23.362501970853884
23	20.574545057618238	29.730265905007442	27.942506510315145	21.752682527059175
24	20.54985523969915	25.888053766626644	34.74840642385747	18.81368456981674
25	19.60267585647679	26.947420217235745	32.56119176451631	20.888712161771153
26	19.456789353403988	31.130048634609754	28.676920304732995	20.73624170725326
27	19.248398627072863	31.26623247239505	28.737215439019614	20.748153461512473
28	18.07069431233226	29.584509348344746	32.90143478162939	19.443361557693606
29	18.767337016880905	26.02761621107455	33.12537576170254	22.079671010342004
30	20.006895822829332	28.969689566689365	30.960858408658936	20.062556201822368
31	24.045630248752083	27.75165854934758	28.220288619639927	19.98242258226041
32	25.41730123830266	26.564078307439342	28.129542709919765	19.889077744338234
33	24.600717997231275	27.395172230971948	28.079383395620976	19.9247263761758
34	19.88266705477328	27.159666020399854	29.62973069905971	23.32793622576716
35	19.90155259970788	27.008581660923035	31.063472757168277	22.026392982200807
36	24.5797966252051	27.055232422149107	28.245498223199416	20.11947272944638
37	20.248509514676147	30.5854865453487	28.51604664902861	20.649957290946546
38	19.974929005944613	30.62148170094653	28.639149214863792	20.764440078245062
39	20.28537097967465	30.2656451146214	28.15691808698093	21.292065818723025
40	23.593286795192327	26.86555397887245	28.475633315487357	21.065525910447867
41	19.144961284632885	26.3044453800586	28.80340147723079	25.747191858077727
42	20.652686165558656	26.620128525662683	27.84773226188187	24.879453046896792
43	20.636269602415965	25.89247194457006	29.60880932703353	23.862449125980444
44	20.17114808519633	27.975859422240934	29.987343219656214	21.86564927290652
45	19.462680257328543	30.670038342854074	27.470021363189822	22.39726003662756
46	20.831189217566674	30.221506650657272	27.979584552663816	20.96771957911224
47	19.99953219292364	27.94588511697776	28.93798264262485	23.116600047473757
48	20.62704340729883	26.284910103073493	31.69371284615558	21.3943336434721
49	20.761407995342722	26.280361978719974	31.561817239903593	21.396412786033707
50	19.722356500179323	29.839594151371973	29.317296213708133	21.120753134740568
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2077.0
1	2682.5
2	3288.0
3	9203.5
4	15119.0
5	11592.0
6	8065.0
7	8588.5
8	9112.0
9	9694.5
10	10277.0
11	10655.0
12	11033.0
13	10997.0
14	10961.0
15	11123.0
16	11285.0
17	11029.5
18	10774.0
19	11542.0
20	12310.0
21	13359.0
22	14408.0
23	16108.5
24	17809.0
25	19768.0
26	21727.0
27	27508.5
28	33290.0
29	38140.0
30	42990.0
31	51605.5
32	60221.0
33	68154.0
34	76087.0
35	86821.0
36	97555.0
37	102732.5
38	107910.0
39	116382.5
40	124855.0
41	143816.0
42	162777.0
43	176644.0
44	190511.0
45	200093.5
46	209676.0
47	203104.5
48	196533.0
49	188560.5
50	180588.0
51	158673.5
52	136759.0
53	121627.0
54	106495.0
55	96956.0
56	87417.0
57	82872.0
58	78327.0
59	73685.0
60	69043.0
61	63255.0
62	57467.0
63	50603.5
64	43740.0
65	36600.5
66	29461.0
67	24426.5
68	19392.0
69	16900.0
70	14408.0
71	11793.0
72	9178.0
73	8093.0
74	7008.0
75	5852.5
76	4697.0
77	3412.0
78	2127.0
79	1520.5
80	914.0
81	793.0
82	672.0
83	436.5
84	201.0
85	129.0
86	57.0
87	32.5
88	8.0
89	17.0
90	26.0
91	15.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2308644.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.96535336202816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08359401852272	18.161826817745506
2	6.816445481888649	3.1308415832914633
3	2.4145664744957003	1.6635411690870092
4	1.3727088240140306	1.2609897282662537
5	0.9445171743414259	1.0845585332627603
6	0.7218139051545415	0.9946026836099716
7	0.5791413004028385	0.9310129227206981
8	0.46998700278833344	0.8634734075675674
9	0.4103995080196273	0.8482472749145925
>10	5.171280096678557	27.995640470784288
>50	1.4308622826284372	22.966176254575796
>100	0.5814335692163584	18.967987125531398
>500	0.0017207798023019482	0.27333502050856096
>1k	0.0013383842906792929	0.5102692808562227
>5k	1.9119775581132755E-4	0.3474977272778717
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7914	0.34279862984505194	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2646	0.11461273370861856	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.086890832887184	0.0
2	0.0	0.0	0.0	0.3055473256162492	0.0
3	0.0	0.0	0.0	0.4244482908581834	0.0
4	0.0	0.0	0.0	0.6745951303015969	0.0
5	0.0	0.0	0.0	1.0763894303322643	0.0
6	0.0	0.0	0.0	1.6845819450725188	0.0
7	0.0	0.0	0.0	1.9829389026632083	0.0
8	0.0	0.0	0.0	2.8234755986631113	0.0
9	0.0	0.0	0.0	3.149511141605202	0.0
10	0.0	0.0	0.0	3.6859732379699945	0.0
11	0.0	0.0	0.0	4.11367018908069	0.0
12	0.0	0.0	0.0	4.497531884517492	0.0
13	0.0	0.0	0.0	4.693491070949007	0.0
14	0.0	0.0	0.0	4.781767998877263	0.0
15	0.0	0.0	0.0	4.885162025847207	0.0
16	0.0	0.0	0.0	5.063318554094958	0.0
17	0.0	0.0	0.0	5.2638258648799905	0.0
18	0.0	0.0	0.0	5.548191925649862	0.0
19	0.0	0.0	0.0	5.680174162841911	0.0
20	0.0	0.0	0.0	5.82159917250126	0.0
21	0.0	0.0	0.0	5.997849820067538	0.0
22	0.0	0.0	0.0	6.190646977186608	0.0
23	0.0	0.0	0.0	6.416797046231467	0.0
24	0.0	0.0	0.0	6.593567479438146	0.0
25	0.0	0.0	0.0	6.7445652079748974	0.0
26	0.0	0.0	0.0	6.894350103350711	0.0
27	0.0	0.0	0.0	7.041406124114415	0.0
28	0.0	0.0	0.0	7.198251441105688	0.0
29	0.0	0.0	0.0	7.3617673404821184	0.0
30	0.0	0.0	0.0	7.5940248908017	0.0
31	0.0	0.0	0.0	7.778548793144374	0.0
32	0.0	0.0	0.0	7.965844885569191	0.0
33	0.0	0.0	0.0	8.143568259116607	0.0
34	0.0	0.0	0.0	8.313581478998062	0.0
35	0.0	0.0	0.0	8.519763116357481	0.0
36	0.0	0.0	0.0	8.708358672883303	0.0
37	0.0	0.0	0.0	8.893965461976814	0.0
38	0.0	0.0	0.0	9.096421968913354	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6580	0.0	41.12462	1
TACGGGA	620	0.0	40.806454	4
TAATGCG	65	0.0	40.615383	1
TATCACG	50	1.3496901E-9	39.600002	1
ATTAGCG	150	0.0	38.13333	1
AGGGTAC	545	0.0	37.944954	6
GTTACGG	215	0.0	37.860466	2
TGTACGG	390	0.0	37.794872	2
CGTAAGG	545	0.0	37.541283	2
GCGATAA	170	0.0	37.52941	9
CTAGACG	65	1.0913936E-11	37.230766	1
TTAGGGA	2685	0.0	36.707634	4
AGGGCGA	2560	0.0	36.695312	6
TCGTAAG	210	0.0	36.666668	1
TTACGCG	90	0.0	36.666668	1
TACGGTC	60	1.9826984E-10	36.666664	4
ACGGGAT	1100	0.0	36.4	5
TCGTCCC	780	0.0	36.384617	38
ACCGGGT	85	0.0	36.235294	4
TAGCACG	85	0.0	36.235294	1
>>END_MODULE