FastQCFastQC Report
Thu 26 May 2016
SRR1546632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546632_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1356817
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42310.31183276742552607No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40760.3004089718805115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15780.11630160883892228No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG207.856986E-444.01
CTTAACG600.044.01
AACGTCA254.443265E-544.038
GGTCGCT254.443265E-544.09
GGTCGAC207.856986E-444.09
CGACGGT454.802132E-1044.028
TCCGATA207.856986E-444.031
CACGCTA254.443265E-544.013
CCTAACG207.856986E-444.01
GCGCAAA207.856986E-444.014
CGTAGAC207.856986E-444.016
AACGCGA207.856986E-444.033
ACGTGCG207.856986E-444.01
TTAACGC254.443265E-544.014
TCCGTCG207.856986E-444.012
TCCCGTA207.856986E-444.013
ACTCGAT408.314601E-944.012
CCGACTC207.856986E-444.016
CGTAATG207.856986E-444.035
TTCGGAC254.443265E-544.014
Produced by FastQC (version 0.11.5)