FastQCFastQC Report
Thu 26 May 2016
SRR1546631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546631_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2308249
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97110.42070851108350965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA207.8581314E-444.00000421
ACCGAAC207.8581314E-444.00000424
CTACGCG351.4469333E-744.01
CGTTTTT86100.041.444831
CGGTCTA800.041.25000431
TATCGCG557.8216544E-1140.01
GCGATCG501.3496901E-939.69
GCGCGAC1350.039.111119
TAACGCG452.3526809E-839.1111071
CGGGTCA850.038.823536
ACGCGAG800.038.5000041
CGTTCGG404.1269413E-738.5000042
CGTACTA404.1269413E-738.50000438
GCGTAAG1600.038.5000041
GGCGCGA1400.037.7142878
TTAATCG3750.037.54666520
CGAAAGG3700.037.459462
TACGGGA10250.037.346344
GCGATAC1950.037.230779
TAGGGAC30150.037.140965
Produced by FastQC (version 0.11.5)