##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546631_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2308249
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.070556945979398	31.0	31.0	33.0	30.0	34.0
2	31.454162440880513	31.0	31.0	34.0	30.0	34.0
3	31.59914160040793	31.0	31.0	34.0	30.0	34.0
4	35.36979632613293	37.0	35.0	37.0	33.0	37.0
5	35.164835769451216	37.0	35.0	37.0	32.0	37.0
6	35.2440373633867	37.0	35.0	37.0	32.0	37.0
7	35.7775001743746	37.0	35.0	37.0	35.0	37.0
8	35.82939059001	37.0	35.0	37.0	35.0	37.0
9	37.60570371740657	39.0	37.0	39.0	35.0	39.0
10	36.86302214362489	39.0	37.0	39.0	32.0	39.0
11	36.4435418362577	38.0	35.0	39.0	32.0	39.0
12	36.09501791184573	38.0	35.0	39.0	31.0	39.0
13	35.99845250664031	38.0	35.0	39.0	31.0	39.0
14	36.98288226270216	39.0	35.0	41.0	31.0	41.0
15	37.150546149917105	39.0	35.0	41.0	32.0	41.0
16	37.17623315335564	39.0	35.0	41.0	32.0	41.0
17	37.082509296007494	39.0	35.0	41.0	32.0	41.0
18	37.00502762050368	38.0	35.0	40.0	31.0	41.0
19	36.98381500436045	38.0	35.0	40.0	31.0	41.0
20	36.87195315583371	38.0	35.0	40.0	31.0	41.0
21	36.78714341477024	38.0	35.0	40.0	31.0	41.0
22	36.71248812411486	38.0	35.0	40.0	31.0	41.0
23	36.50398288919436	38.0	35.0	40.0	30.0	41.0
24	36.47799132589248	38.0	35.0	40.0	30.0	41.0
25	36.34616499346474	38.0	35.0	40.0	30.0	41.0
26	36.26065038910447	38.0	35.0	40.0	30.0	41.0
27	36.10757418285462	38.0	35.0	40.0	30.0	41.0
28	36.07816444413059	38.0	35.0	40.0	30.0	41.0
29	35.97991594494355	38.0	34.0	40.0	30.0	41.0
30	35.96020836573524	38.0	35.0	40.0	30.0	41.0
31	35.9152812369896	38.0	34.0	40.0	30.0	41.0
32	35.73313321049852	38.0	34.0	40.0	29.0	41.0
33	35.54837454711342	38.0	34.0	40.0	27.0	41.0
34	35.457607909718575	38.0	34.0	40.0	27.0	41.0
35	35.244430735158986	38.0	34.0	40.0	26.0	41.0
36	35.08675233911073	38.0	34.0	40.0	25.0	41.0
37	35.167384021394575	38.0	34.0	40.0	25.0	41.0
38	35.14096312832801	38.0	34.0	40.0	26.0	41.0
39	35.07948275944233	38.0	34.0	40.0	25.0	41.0
40	35.07680150624998	38.0	34.0	40.0	25.0	41.0
41	35.10171606269514	38.0	34.0	40.0	25.0	41.0
42	34.882792107783864	38.0	34.0	40.0	24.0	41.0
43	34.952106553495746	38.0	34.0	40.0	25.0	41.0
44	34.778586279036624	38.0	34.0	40.0	24.0	41.0
45	34.618999943247026	38.0	33.0	40.0	24.0	41.0
46	34.707298476030964	38.0	33.0	40.0	24.0	41.0
47	34.58331055271766	37.0	33.0	40.0	24.0	41.0
48	34.63747065416253	37.0	33.0	40.0	24.0	41.0
49	34.565805942079905	37.0	33.0	40.0	24.0	41.0
50	34.08240748723383	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	5.0
14	16.0
15	27.0
16	151.0
17	403.0
18	938.0
19	1940.0
20	3497.0
21	5683.0
22	8532.0
23	12721.0
24	18213.0
25	26133.0
26	35061.0
27	41649.0
28	47188.0
29	54170.0
30	65355.0
31	80267.0
32	97948.0
33	124474.0
34	178110.0
35	228831.0
36	193093.0
37	239308.0
38	338765.0
39	505768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.115771738664243	25.203823331018448	30.17642377403824	14.503981156279067
2	30.00733456399201	26.29720623728203	30.490471348628333	13.204987850097627
3	26.947915931080225	26.390523725993166	33.21851325398603	13.443047088940578
4	23.800161940934448	29.487633266601655	32.35534814484919	14.356856647614707
5	21.982030534833978	33.30320948909758	31.21755928411536	13.49720069195308
6	21.35384873988898	41.840200082400116	27.091574609151785	9.714376568559112
7	90.70845476376249	2.7571115594548075	5.642415527960805	0.8920181488218992
8	91.59384451157567	1.9859642525568082	5.285348331137586	1.1348429047299489
9	85.47998937722923	5.511667068847425	6.925639304945004	2.082704248978338
10	37.626053341732195	41.928188856574835	11.86024558009123	8.585512221601741
11	25.858172146939083	25.200184208896008	33.96500984079274	14.976633803372167
12	27.327121120814958	23.90093096542011	32.1851325398603	16.586815373904635
13	20.81001659699625	31.400208556355924	32.10255912598684	15.687215720660985
14	17.856219151400044	33.4202679173694	31.2354949574331	17.488017973797454
15	16.249763348754836	27.401723124324974	39.37231208591447	16.976201441005713
16	19.074631896298882	25.22648119851888	38.61546133021177	17.083425574970466
17	19.51492235023171	24.291226813051797	32.52352757436481	23.67032326235168
18	20.521659491675294	25.40783078428714	34.96130616757551	19.109203556462063
19	23.42533236232313	26.37258805267543	31.526927987405173	18.675151597596273
20	24.732210433103187	26.716311801716365	30.750928517677252	17.800549247503195
21	22.25271190413166	27.779325367410536	32.12482708754558	17.843135640912227
22	21.97713504912165	26.329568430442297	30.554935797654416	21.13836072278164
23	18.7571618139984	29.323829448209445	31.29580040974782	20.62320832804433
24	18.602455800912292	27.191043947165145	36.677585477130066	17.528914774792494
25	18.297592677393123	28.235428673423012	34.83861576459039	18.628362884593475
26	18.369422016428906	31.927967909874543	30.894240612689533	18.808369461007025
27	17.708531445264352	32.06456495811327	31.80341462294579	18.423488973676584
28	17.224744817391883	29.88195814229747	35.42425448900877	17.469042551301875
29	17.689729314298415	26.794509604466416	35.63835617387899	19.877404907356183
30	18.638976990783924	29.417276905567814	33.00640442170667	18.937341681941593
31	22.12135692466454	28.331215566431524	31.23848423631939	18.308943272584543
32	23.07606328433371	27.869393639940927	31.40999952778058	17.644543547944785
33	22.521963618309808	28.404301269057196	30.749022310851217	18.324712801781786
34	19.549060781570795	28.565679006034443	31.34882761781766	20.5364325945771
35	19.012506882922942	28.394726912044582	32.66809603296698	19.924670172065493
36	23.08884353464466	28.073271124562382	29.936458328369252	18.901427012423703
37	19.564873633650443	31.00826643919265	31.458174573020504	17.968685354136404
38	19.754931118783112	30.552163133180173	29.921988485644313	19.770917262392402
39	20.237764643242564	30.870023121422342	30.20969574772912	18.682516487605973
40	22.2658820603843	28.596611544075184	29.611450064529432	19.526056331011084
41	18.630312414301923	27.840800537550326	30.936610391686514	22.59227665646124
42	20.42283999689808	28.232006165712626	29.686615265510785	21.65853857187851
43	20.369856111710654	27.811211008864294	30.622476171331602	21.19645670809345
44	18.993011585838442	30.076997758907293	30.99435979393904	19.935630861315225
45	18.338142895328883	32.31761391427008	29.128139988363472	20.216103202037562
46	20.19422514642051	31.113021168860033	29.764899714025656	18.927853970693803
47	19.502791943156915	29.201398982518782	30.643444446418044	20.652364627906262
48	20.118756685262294	28.263241963930234	32.06274539705205	19.55525595375542
49	20.277015174706023	27.216864385081507	32.33628607658879	20.169834363623682
50	19.255764867655092	30.621696359448226	30.739664568250653	19.382874204646033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1499.0
1	2050.0
2	2601.0
3	10905.5
4	19210.0
5	14751.0
6	10292.0
7	10666.5
8	11041.0
9	11614.5
10	12188.0
11	12247.0
12	12306.0
13	12433.5
14	12561.0
15	12246.0
16	11931.0
17	11868.0
18	11805.0
19	12137.0
20	12469.0
21	14834.0
22	17199.0
23	19688.5
24	22178.0
25	26880.5
26	31583.0
27	38822.5
28	46062.0
29	55135.5
30	64209.0
31	73302.5
32	82396.0
33	93582.5
34	104769.0
35	112213.5
36	119658.0
37	127819.0
38	135980.0
39	145726.5
40	155473.0
41	168811.5
42	182150.0
43	193844.0
44	205538.0
45	205115.0
46	204692.0
47	191706.5
48	178721.0
49	170128.0
50	161535.0
51	142484.0
52	123433.0
53	108680.0
54	93927.0
55	83789.5
56	73652.0
57	65653.0
58	57654.0
59	51317.5
60	44981.0
61	39597.0
62	34213.0
63	27780.0
64	21347.0
65	16970.5
66	12594.0
67	9429.5
68	6265.0
69	5295.0
70	4325.0
71	3357.5
72	2390.0
73	1984.0
74	1578.0
75	1252.0
76	926.0
77	676.0
78	426.0
79	342.0
80	258.0
81	176.0
82	94.0
83	92.0
84	90.0
85	61.5
86	33.0
87	20.0
88	7.0
89	6.5
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2308249.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.409927287056895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.91448873901365	15.90229049771482
2	6.231541756486334	2.543706282722898
3	2.3060268287322767	1.4119751968928451
4	1.4246077348026476	1.1630456111960346
5	0.9925043974036519	1.0128471291546377
6	0.797623037341637	0.9767656916774565
7	0.6402898263141539	0.9147788158399917
8	0.5573897962100888	0.9101028168956298
9	0.4781003025324985	0.8782193169547379
>10	6.262435653305347	30.32363836978837
>50	1.7481925915834564	25.16370283245332
>100	0.6459412283468241	18.206519081121208
>500	2.1452698183819136E-4	0.0302115126929619
>1k	4.290539636763827E-4	0.13700264234243156
>5k	2.1452698183819136E-4	0.4251942025526855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9711	0.42070851108350965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014989717313859986	0.0
2	0.0	0.0	0.0	0.08625585887830992	0.0
3	0.0	0.0	0.0	0.13871986947681988	0.0
4	0.0	0.0	0.0	0.2199069511131598	0.0
5	0.0	0.0	0.0	0.35589747899814966	0.0
6	0.0	0.0	0.0	0.5839924548867995	0.0
7	0.0	0.0	0.0	0.6994912593918594	0.0
8	0.0	0.0	0.0	0.9767577068158592	0.0
9	0.0	0.0	0.0	1.125961713835899	0.0
10	0.0	0.0	0.0	1.357219260140479	0.0
11	0.0	0.0	0.0	1.5689381864781486	0.0
12	0.0	0.0	0.0	1.7481649510083184	0.0
13	8.664576481999992E-5	0.0	0.0	1.8429120948389883	0.0
14	8.664576481999992E-5	0.0	0.0	1.8916503375502383	0.0
15	8.664576481999992E-5	0.0	0.0	1.9384823734354482	0.0
16	8.664576481999992E-5	0.0	0.0	2.025734658609188	0.0
17	8.664576481999992E-5	0.0	0.0	2.127890015331968	0.0
18	8.664576481999992E-5	0.0	0.0	2.2652235525716677	0.0
19	8.664576481999992E-5	0.0	0.0	2.3362297568416577	0.0
20	8.664576481999992E-5	0.0	0.0	2.411871509529518	0.0
21	8.664576481999992E-5	0.0	0.0	2.506575330477778	0.0
22	8.664576481999992E-5	0.0	0.0	2.6116333203220274	0.0
23	8.664576481999992E-5	0.0	0.0	2.7309445384791675	0.0
24	8.664576481999992E-5	0.0	0.0	2.8251284848385074	0.0
25	8.664576481999992E-5	0.0	0.0	2.9033262875885573	0.0
26	8.664576481999992E-5	0.0	0.0	2.9834302971646474	0.0
27	8.664576481999992E-5	0.0	0.0	3.075318130756257	0.0
28	8.664576481999992E-5	0.0	0.0	3.1660795694052073	0.0
29	8.664576481999992E-5	0.0	0.0	3.266502010831587	0.0
30	8.664576481999992E-5	0.0	0.0	3.393221441880837	0.0
31	8.664576481999992E-5	0.0	0.0	3.507853788737697	0.0
32	8.664576481999992E-5	0.0	0.0	3.612348581110617	0.0
33	8.664576481999992E-5	0.0	0.0	3.7146772293630366	0.0
34	8.664576481999992E-5	0.0	0.0	3.8231577269176764	0.0
35	8.664576481999992E-5	0.0	0.0	3.9543394148551565	0.0
36	8.664576481999992E-5	0.0	0.0	4.0734340186002465	0.0
37	8.664576481999992E-5	0.0	0.0	4.193828308817636	0.0
38	8.664576481999992E-5	0.0	0.0	4.321110937338216	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	20	7.8581314E-4	44.000004	21
ACCGAAC	20	7.8581314E-4	44.000004	24
CTACGCG	35	1.4469333E-7	44.0	1
CGTTTTT	8610	0.0	41.44483	1
CGGTCTA	80	0.0	41.250004	31
TATCGCG	55	7.8216544E-11	40.0	1
GCGATCG	50	1.3496901E-9	39.6	9
GCGCGAC	135	0.0	39.11111	9
TAACGCG	45	2.3526809E-8	39.111107	1
CGGGTCA	85	0.0	38.82353	6
ACGCGAG	80	0.0	38.500004	1
CGTTCGG	40	4.1269413E-7	38.500004	2
CGTACTA	40	4.1269413E-7	38.500004	38
GCGTAAG	160	0.0	38.500004	1
GGCGCGA	140	0.0	37.714287	8
TTAATCG	375	0.0	37.546665	20
CGAAAGG	370	0.0	37.45946	2
TACGGGA	1025	0.0	37.34634	4
GCGATAC	195	0.0	37.23077	9
TAGGGAC	3015	0.0	37.14096	5
>>END_MODULE