FastQCFastQC Report
Thu 26 May 2016
SRR1546630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546630_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1570607
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57890.36858361130441925No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32990.21004617959807897No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTC207.8573625E-444.00000418
ATCCGCG207.8573625E-444.0000041
CCCGTTA207.8573625E-444.00000431
GTCGTTA207.8573625E-444.00000434
CGCACCA207.8573625E-444.00000418
CACGTCG207.8573625E-444.00000428
ACCGCGA254.443588E-544.011
TAGCGGA454.802132E-1044.03
CGCGAGT254.443588E-544.013
TCGATAG254.443588E-544.01
CGTAATC254.443588E-544.041
GCGTAAT254.443588E-544.040
GCGTAAG1550.044.01
CGACGGT1250.042.23999828
GCGTTAG1100.042.01
CGTTTTT58850.041.9813081
CGTAAGG3150.041.2063522
GCCGATC650.040.6153839
TATAACG1050.039.809521
ACGGGAT9950.039.7989965
Produced by FastQC (version 0.11.5)