FastQCFastQC Report
Thu 26 May 2016
SRR1546629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546629_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2949796
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112860.38260272913787935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG351.4471152E-744.011
CGGTCTA750.041.06666631
CGTTTTT105800.040.880911
TACGGGA13400.040.05974
ACGGGAT21700.038.9308785
AGGGCGA29600.038.7972956
CGTTAGG5100.038.392162
TCGTAAG1550.038.3225821
AATCGCG357.2940857E-637.7142871
AGGGATC48000.037.445836
CGTAAGG5600.037.321432
AAGGGAT68800.037.0610475
AACGGGA14250.036.8982434
GGGCGAT62700.036.4912267
TAAGGGA50100.036.4910164
TAGGGAT63900.036.4600945
AGGGATT84100.036.099886
ATAGGGA45100.036.04
CGACAAT2700.035.85185220
AAGGGCG12650.035.8260885
Produced by FastQC (version 0.11.5)