##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546629_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2949796
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03256259076899	31.0	31.0	33.0	30.0	34.0
2	31.416723054746836	31.0	31.0	34.0	30.0	34.0
3	31.572140581924987	31.0	31.0	34.0	30.0	34.0
4	35.341971444805	37.0	35.0	37.0	33.0	37.0
5	35.14700982712025	37.0	35.0	37.0	32.0	37.0
6	35.21990300346194	37.0	35.0	37.0	32.0	37.0
7	35.73258320236383	37.0	35.0	37.0	35.0	37.0
8	35.7756193987652	37.0	35.0	37.0	35.0	37.0
9	37.54828299990915	39.0	37.0	39.0	35.0	39.0
10	36.806452717408256	39.0	37.0	39.0	32.0	39.0
11	36.393556367965786	38.0	35.0	39.0	32.0	39.0
12	36.05435731826879	38.0	35.0	39.0	31.0	39.0
13	35.96970231161748	38.0	35.0	39.0	31.0	39.0
14	36.954878235647485	39.0	35.0	41.0	31.0	41.0
15	37.109353324772286	39.0	35.0	41.0	32.0	41.0
16	37.13355499837955	39.0	35.0	41.0	32.0	41.0
17	37.02912811597819	39.0	35.0	41.0	31.0	41.0
18	36.95073218622576	38.0	35.0	40.0	31.0	41.0
19	36.938162503440914	38.0	35.0	40.0	31.0	41.0
20	36.81104150931115	38.0	35.0	40.0	31.0	41.0
21	36.70971111222607	38.0	35.0	40.0	31.0	41.0
22	36.6362338276952	38.0	35.0	40.0	31.0	41.0
23	36.434510725487456	38.0	35.0	40.0	30.0	41.0
24	36.41381336200876	38.0	35.0	40.0	30.0	41.0
25	36.277182218702585	38.0	35.0	40.0	30.0	41.0
26	36.163240441033885	38.0	35.0	40.0	30.0	41.0
27	35.999875584616696	38.0	34.0	40.0	30.0	41.0
28	35.96214822991149	38.0	34.0	40.0	30.0	41.0
29	35.870369001788596	38.0	34.0	40.0	29.0	41.0
30	35.84279217952699	38.0	34.0	40.0	29.0	41.0
31	35.80482989332144	38.0	34.0	40.0	29.0	41.0
32	35.62292307671446	38.0	34.0	40.0	28.0	41.0
33	35.440132131171104	38.0	34.0	40.0	27.0	41.0
34	35.35966656677275	38.0	34.0	40.0	27.0	41.0
35	35.16183898818766	38.0	34.0	40.0	26.0	41.0
36	34.99173502167608	38.0	34.0	40.0	25.0	41.0
37	35.083046420837235	38.0	34.0	40.0	25.0	41.0
38	35.04875354092283	38.0	34.0	40.0	25.0	41.0
39	34.96009452857079	38.0	34.0	40.0	25.0	41.0
40	34.95237162163078	38.0	34.0	40.0	24.0	41.0
41	34.97147701061362	38.0	34.0	40.0	25.0	41.0
42	34.7181791554399	38.0	33.0	40.0	24.0	41.0
43	34.792802281920515	38.0	33.0	40.0	24.0	41.0
44	34.61861260914314	38.0	33.0	40.0	24.0	41.0
45	34.45096338865467	37.0	33.0	40.0	23.0	41.0
46	34.52530446173227	37.0	33.0	40.0	23.0	41.0
47	34.39779700019933	37.0	33.0	40.0	23.0	41.0
48	34.45821507656801	37.0	33.0	40.0	23.0	41.0
49	34.36449300222795	37.0	33.0	40.0	24.0	41.0
50	33.89850450675233	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	8.0
14	16.0
15	66.0
16	210.0
17	649.0
18	1371.0
19	2919.0
20	4865.0
21	7896.0
22	12022.0
23	17483.0
24	24846.0
25	34278.0
26	45038.0
27	54766.0
28	62294.0
29	72218.0
30	86788.0
31	104649.0
32	128021.0
33	160411.0
34	227513.0
35	292052.0
36	248143.0
37	307268.0
38	432966.0
39	621037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.0460791186916	24.506169240177964	30.42712106193106	14.020630579199375
2	30.049739032800915	26.307683649988	29.998277847010435	13.644299470200652
3	27.69957651308769	26.1664874452335	32.590796109290274	13.543139932388545
4	24.13573684417499	29.276397418668953	32.19059894311335	14.397266794042707
5	22.11986862820344	33.713416114199084	30.648288898622145	13.518426358975333
6	21.473756151272834	42.10599648246862	26.61736608226467	9.802881283993877
7	90.06117711190875	3.3163649282865664	5.614252646623698	1.0082053131809792
8	90.73071493757534	2.685372140988733	5.255821080508618	1.3280918409273048
9	84.8718691055246	5.955123676349144	6.921427786870685	2.251579431255585
10	37.952522818527115	39.90031853050177	12.450454200900673	9.696704450070445
11	26.45867714241934	25.95935447739437	32.30403051600857	15.277937864177726
12	27.379147574950945	24.713505611913504	31.24348260015269	16.663864212982865
13	21.384258436854616	30.48353174253406	31.88213693421511	16.25007288639621
14	18.291739496561796	32.704532788030086	31.162866855877493	17.840860859530626
15	16.736411602700663	26.92037008660938	39.34116121928432	17.002057091405643
16	19.58030318028772	24.998067662984152	38.03361317189392	17.388015984834205
17	19.615932762808004	24.84178566924628	32.11608531573031	23.42619625221541
18	20.345339135316475	25.957896749470134	34.17507515773972	19.52168895747367
19	24.3057825015696	26.48820460804747	30.340810008556524	18.865202881826406
20	25.94901477932711	26.56976957050589	29.777279513566363	17.703936136600632
21	22.88592160271422	28.35697790626877	31.049130177137673	17.707970313879333
22	22.59932551267952	26.034647819713637	29.886812511780477	21.47921415582637
23	19.51741747564916	29.591368352252157	30.60492996803847	20.286284204060212
24	19.068301672386838	26.249374533018553	36.77376333821051	17.908560456384105
25	19.063521680821317	27.836806341862285	33.81128728901931	19.28838468829709
26	18.995144070979826	31.720871545015317	30.23253133437024	19.05145304963462
27	18.612304037296138	31.587574191571218	31.100150654485937	18.69997111664671
28	17.76570311980896	29.85318306757484	34.564695321303574	17.816418491312618
29	18.750279680357558	26.412945166377604	34.65131148052272	20.185463672742117
30	19.720584067508398	29.905593471548542	31.946514267427307	18.427308193515753
31	22.959892819706855	28.667846861274477	29.65123689909404	18.72102341992463
32	23.6478725986475	27.82331388340075	30.427765174269677	18.101048343682073
33	23.601327007020146	28.951256290265498	29.27510919399172	18.17230750872264
34	20.427988918555723	28.04285448892059	30.735549170179905	20.793607422343783
35	19.855271347577936	28.178660490420356	31.905663984899295	20.060404177102416
36	23.550713337464693	28.084891294177634	29.51573600343888	18.84865936491879
37	20.011112632873594	31.154595097423687	30.362743728718865	18.47154854098385
38	19.38191657999401	31.16001920132782	29.833385088324754	19.62467913035342
39	20.393410256166867	30.84538727423862	29.69317200240288	19.06803046719163
40	21.779133201075602	28.263039206779045	29.945765741088536	20.01206185105682
41	18.93086843971583	27.627368129863893	31.0826579193951	22.359105511025167
42	20.004196900395822	28.03451492916798	29.49705674561902	22.464231424817175
43	20.095559150531088	28.33511198740523	29.86952318058605	21.699805681477635
44	19.610678162150876	29.50753204628388	30.87460963402215	20.007180157543097
45	19.157392579012246	31.694564641080262	28.815348586817528	20.332694193089964
46	19.78658863189183	31.4012901231136	29.37321767335775	19.438903571636818
47	19.82323523389414	28.89976120382562	30.06729956919055	21.209703993089693
48	20.319472939823633	27.66621827407726	31.93647967520466	20.07782911089445
49	20.477653369927953	27.3207367560333	32.360034388818754	19.841575485219995
50	19.78852096890768	30.94844524841718	29.673577427049192	19.58945635562595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2558.0
1	3241.0
2	3924.0
3	13554.0
4	23184.0
5	18144.5
6	13105.0
7	13450.5
8	13796.0
9	14559.0
10	15322.0
11	15741.0
12	16160.0
13	16328.5
14	16497.0
15	15875.5
16	15254.0
17	16344.0
18	17434.0
19	18003.0
20	18572.0
21	20820.5
22	23069.0
23	25193.0
24	27317.0
25	34388.0
26	41459.0
27	49712.5
28	57966.0
29	66689.0
30	75412.0
31	88251.0
32	101090.0
33	114829.5
34	128569.0
35	138886.5
36	149204.0
37	159767.5
38	170331.0
39	181374.5
40	192418.0
41	205780.0
42	219142.0
43	233441.0
44	247740.0
45	247409.0
46	247078.0
47	235349.0
48	223620.0
49	210160.5
50	196701.0
51	174927.0
52	153153.0
53	137720.0
54	122287.0
55	109526.0
56	96765.0
57	89034.5
58	81304.0
59	78086.5
60	74869.0
61	66228.0
62	57587.0
63	48907.5
64	40228.0
65	32623.0
66	25018.0
67	19979.5
68	14941.0
69	12660.5
70	10380.0
71	8537.0
72	6694.0
73	5516.5
74	4339.0
75	3559.5
76	2780.0
77	2073.5
78	1367.0
79	1022.0
80	677.0
81	509.0
82	341.0
83	210.5
84	80.0
85	64.0
86	48.0
87	28.0
88	8.0
89	6.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2949796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.89150133737376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73104601043819	15.660787070094662
2	6.090943617085083	2.423160262102322
3	2.2534483177941294	1.344734106813137
4	1.2878177774111847	1.0246651616667313
5	0.8935329590681541	0.888685602514586
6	0.7327011335620036	0.874471534688632
7	0.620019476616703	0.8633182773823248
8	0.5015193380180779	0.7980778066324319
9	0.4392595729775395	0.7863779145003257
>10	5.922768800381181	28.08911504434559
>50	1.748155367546566	24.461440117971474
>100	0.7765413608482219	22.022348259154025
>500	0.0015551087905064898	0.19821466444018093
>1k	5.183695968354966E-4	0.176698732423612
>5k	0.0	0.0
>10k+	1.7278986561183217E-4	0.38790544526996396
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11286	0.38260272913787935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01813684742944936	0.0
2	0.0	0.0	0.0	0.07827659946653938	0.0
3	0.0	0.0	0.0	0.11994049758017164	0.0
4	0.0	0.0	0.0	0.19350490677999427	0.0
5	0.0	0.0	0.0	0.3033091101893148	0.0
6	0.0	0.0	0.0	0.5381050079395321	0.0
7	0.0	0.0	0.0	0.6544181360338138	0.0
8	0.0	0.0	0.0	0.9738300546885276	0.0
9	0.0	0.0	0.0	1.1327223984302643	0.0
10	0.0	0.0	0.0	1.3739255189172404	0.0
11	0.0	0.0	0.0	1.5606502958170667	0.0
12	0.0	0.0	0.0	1.7279839012596125	0.0
13	0.0	0.0	0.0	1.8209055812673147	0.0
14	0.0	0.0	0.0	1.8679257819862798	0.0
15	0.0	0.0	0.0	1.9059284099646212	0.0
16	0.0	0.0	0.0	1.98054373929587	0.0
17	0.0	0.0	0.0	2.0640749394195397	0.0
18	0.0	0.0	0.0	2.1788286376413826	0.0
19	0.0	0.0	0.0	2.2413753357859325	0.0
20	0.0	0.0	0.0	2.3046678482173006	0.0
21	0.0	0.0	0.0	2.3861989100263203	0.0
22	0.0	0.0	0.0	2.4777306634085883	0.0
23	0.0	0.0	0.0	2.5806191343401372	0.0
24	0.0	0.0	0.0	2.661370481212938	0.0
25	0.0	0.0	0.0	2.732358441058297	0.0
26	0.0	0.0	0.0	2.804770228178491	0.0
27	0.0	0.0	0.0	2.8799279679001533	0.0
28	0.0	0.0	0.0	2.9549501050242117	0.0
29	0.0	0.0	0.0	3.04004073502032	0.0
30	0.0	0.0	0.0	3.151472169600881	0.0
31	0.0	0.0	0.0	3.2470720009112495	0.0
32	0.0	0.0	0.0	3.3416548127395926	0.0
33	0.0	0.0	0.0	3.438983577169404	0.0
34	0.0	0.0	0.0	3.5359055338064054	0.0
35	0.0	0.0	0.0	3.64991341774143	0.0
36	0.0	0.0	0.0	3.7584971977723205	0.0
37	0.0	0.0	0.0	3.872166075213337	0.0
38	0.0	0.0	0.0	3.9870214753833824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	35	1.4471152E-7	44.0	11
CGGTCTA	75	0.0	41.066666	31
CGTTTTT	10580	0.0	40.88091	1
TACGGGA	1340	0.0	40.0597	4
ACGGGAT	2170	0.0	38.930878	5
AGGGCGA	2960	0.0	38.797295	6
CGTTAGG	510	0.0	38.39216	2
TCGTAAG	155	0.0	38.322582	1
AATCGCG	35	7.2940857E-6	37.714287	1
AGGGATC	4800	0.0	37.44583	6
CGTAAGG	560	0.0	37.32143	2
AAGGGAT	6880	0.0	37.061047	5
AACGGGA	1425	0.0	36.898243	4
GGGCGAT	6270	0.0	36.491226	7
TAAGGGA	5010	0.0	36.491016	4
TAGGGAT	6390	0.0	36.460094	5
AGGGATT	8410	0.0	36.09988	6
ATAGGGA	4510	0.0	36.0	4
CGACAAT	270	0.0	35.851852	20
AAGGGCG	1265	0.0	35.826088	5
>>END_MODULE