FastQCFastQC Report
Thu 26 May 2016
SRR1546628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546628_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3560240
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210220.5904658112936206No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT210100.042.7120441
ACGGGCC2050.039.7073175
GTTCGCG501.3496901E-939.61
TACGGGA18200.038.5604364
AGGGCGA38000.038.5000046
CGTAAGG6000.038.52
TAGGGCG12600.038.4126975
CGAACGG700.037.7142832
AAGGGAT96200.037.6424145
GGCGATA18300.037.2677578
GGGCGAT77100.036.9520077
AGGGATT125700.036.7541776
ACGTTCG601.9826984E-1036.66666413
CGTTCGA601.9826984E-1036.66666414
TAGGGAT93600.036.6666645
AGGGATC60150.036.5752266
GCGTAAG2550.036.235291
AGGGACC46900.036.213226
TAAGGGA70150.036.1910174
TTAGGGA71400.036.1120454
Produced by FastQC (version 0.11.5)