##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546628_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3560240
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98137035705458	31.0	31.0	33.0	30.0	34.0
2	31.356661910433004	31.0	31.0	34.0	30.0	34.0
3	31.484200222456913	31.0	31.0	34.0	30.0	34.0
4	35.26865548389996	37.0	35.0	37.0	33.0	37.0
5	35.063030582207944	37.0	35.0	37.0	32.0	37.0
6	35.13832157382648	37.0	35.0	37.0	32.0	37.0
7	35.734830797923735	37.0	35.0	37.0	35.0	37.0
8	35.78385614452958	37.0	35.0	37.0	35.0	37.0
9	37.53536643597061	39.0	37.0	39.0	35.0	39.0
10	36.76662135136957	39.0	37.0	39.0	32.0	39.0
11	36.35813428308204	38.0	35.0	39.0	32.0	39.0
12	36.11825551086444	38.0	35.0	39.0	31.0	39.0
13	36.07061293620655	38.0	35.0	39.0	31.0	39.0
14	37.06591128687953	39.0	35.0	41.0	31.0	41.0
15	37.17749898883221	39.0	36.0	41.0	32.0	41.0
16	37.17275015167517	39.0	35.0	41.0	32.0	41.0
17	37.062246084533626	39.0	35.0	41.0	31.0	41.0
18	36.944257690492776	39.0	35.0	40.0	31.0	41.0
19	36.89845993528526	38.0	35.0	40.0	31.0	41.0
20	36.74392456688313	38.0	35.0	40.0	31.0	41.0
21	36.66525880277734	38.0	35.0	40.0	31.0	41.0
22	36.60791744376784	38.0	35.0	40.0	31.0	41.0
23	36.38483079792373	38.0	35.0	40.0	30.0	41.0
24	36.352503763791205	38.0	35.0	40.0	30.0	41.0
25	36.213409489247915	38.0	35.0	40.0	30.0	41.0
26	36.116490180437275	38.0	35.0	40.0	30.0	41.0
27	35.9357509606094	38.0	34.0	40.0	29.0	41.0
28	35.8817737006494	38.0	34.0	40.0	29.0	41.0
29	35.769209660022916	38.0	34.0	40.0	29.0	41.0
30	35.720035727928455	38.0	34.0	40.0	29.0	41.0
31	35.622426297103566	38.0	34.0	40.0	28.0	41.0
32	35.36727552075141	38.0	34.0	40.0	27.0	41.0
33	35.0931737748017	38.0	34.0	40.0	25.0	41.0
34	34.94814029391277	38.0	34.0	40.0	24.0	41.0
35	34.703350335932406	38.0	33.0	40.0	24.0	41.0
36	34.54522447924859	38.0	33.0	40.0	23.0	41.0
37	34.62695211558771	38.0	33.0	40.0	23.0	41.0
38	34.57779587892951	38.0	33.0	40.0	23.0	41.0
39	34.49160309417343	38.0	33.0	40.0	23.0	41.0
40	34.52817956092848	38.0	33.0	40.0	23.0	41.0
41	34.54040514122644	38.0	33.0	40.0	23.0	41.0
42	34.29635108869065	38.0	33.0	40.0	22.0	41.0
43	34.36819484079725	38.0	33.0	40.0	23.0	41.0
44	34.19433408983664	38.0	33.0	40.0	22.0	41.0
45	34.01195481203514	37.0	33.0	40.0	20.0	41.0
46	34.08408225288183	37.0	33.0	40.0	22.0	41.0
47	33.94828438532234	37.0	33.0	40.0	20.0	41.0
48	33.98694048715817	37.0	33.0	40.0	21.0	41.0
49	33.90091594948655	37.0	33.0	40.0	20.0	41.0
50	33.440514122643414	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	4.0
14	18.0
15	83.0
16	289.0
17	821.0
18	1991.0
19	3983.0
20	7092.0
21	11091.0
22	16697.0
23	24246.0
24	35354.0
25	50230.0
26	67499.0
27	79062.0
28	85087.0
29	93588.0
30	108077.0
31	127852.0
32	154945.0
33	190222.0
34	255917.0
35	317810.0
36	302551.0
37	371208.0
38	519211.0
39	735306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.28109340943307	22.99429251960542	29.862986764937194	15.861627306024312
2	30.58279778891311	26.128013841763476	29.820349189942252	13.468839179381165
3	26.253988495157632	26.004567107835424	34.586488551333616	13.154955845673324
4	23.333258432015818	28.623379322742288	34.088291800552774	13.955070444689122
5	21.156719771700786	33.22820933420219	32.65816349459587	12.956907399501159
6	20.500640406264747	41.1424791587084	28.908051142619602	9.448829292407252
7	88.55226052176258	2.6051614497899016	7.899439363638407	0.943138664809114
8	89.15736017796553	1.9961856504055904	7.554940116396647	1.2915140552322315
9	82.94716086555962	5.883648293373481	9.117081994472283	2.0521088465946113
10	32.96960317281981	44.37433993213941	13.890748938273825	8.76530795676696
11	22.349616879760916	26.026110599285442	36.60087522189515	15.023397299058491
12	24.149467451632475	24.475063478866595	34.74993258881424	16.625536480686694
13	20.672201874030964	28.12863739523178	35.16524166910096	16.033919061636297
14	18.105155832191087	30.99105678268881	33.173859065680965	17.72992831943914
15	16.977479046356425	27.401411140821967	39.04792935307732	16.573180459744286
16	19.59640361323956	26.25154483967373	37.976625171336764	16.17542637574995
17	19.888490663550773	25.11943015077635	33.994000404467116	20.998078781205763
18	20.168078556501808	25.701525739837766	35.75472440060221	18.375671303058223
19	22.33166865155158	26.751792014021525	32.91101161719435	18.005527717232546
20	23.72441183740422	27.045480079994604	31.85844212749702	17.371665955104152
21	21.970541311821677	27.678583466283175	33.23702896433948	17.11384625755567
22	21.844145338516505	25.818568411118353	32.36627306024313	19.971013190122015
23	18.542626339797316	28.899624744399254	33.23009122980473	19.327657685998698
24	18.095381210255486	27.271391816282048	36.98573691661236	17.6474900568501
25	18.29545199200054	28.52141990427612	35.00856683819069	18.17456126553266
26	18.69261622811945	30.69217243781318	32.24428690200661	18.370924432060757
27	18.16282048401231	30.238776037570503	33.502320068310006	18.096083410107184
28	17.511909309484754	29.022004134552727	36.004595195829495	17.461491360133024
29	18.302361638541225	26.501331370918813	35.70571085095387	19.490596139586096
30	19.450346044086917	28.56046221603038	33.83667954969328	18.152512190189427
31	21.449958429768778	28.23604588454711	32.02022897332764	18.293766712356472
32	22.18291463496843	28.084511156551244	32.05267060647597	17.67990360200436
33	21.468019009954382	28.885721187335683	31.570736804260385	18.075522998449543
34	19.653618857155696	28.728625036514394	31.922538929959778	19.695217176370132
35	19.10994764397906	28.84993708289329	32.375148866368555	19.664966406759095
36	21.659101633597736	28.81698986585174	30.875109543176865	18.64879895737366
37	19.727349841583713	30.913140687144686	31.048328202593083	18.311181268678517
38	19.263111475630858	30.63829966519111	31.15910725119655	18.939481607981485
39	19.433746039592837	31.08798845021684	30.474967979686763	19.003297530503563
40	21.03720535694223	28.890327618362804	30.902326809428576	19.170140215266386
41	18.505915331550682	29.127811608206187	31.406056895040784	20.960216165202347
42	19.74282632631508	29.364621486191943	30.10518953778397	20.78736264970901
43	19.578680088982765	29.076691459002763	30.907523088331125	20.437105363683347
44	19.335915556254633	30.41084308922994	30.898703458193825	19.354537896321595
45	19.0881794485765	31.090488281688877	29.621345751971777	20.199986517762845
46	20.06325416264072	30.75388176078017	29.98559085904321	19.197273217535894
47	19.44911017234793	29.785520077298163	30.38174392737568	20.383625822978228
48	20.01028020582882	28.556333280902415	31.675476934139272	19.757909579129496
49	19.67406691683707	28.787806440015277	31.51484169606543	20.02328494708222
50	19.052226816169696	30.186251488663686	30.852723411904815	19.908798283261802
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2117.0
1	2845.0
2	3573.0
3	23060.0
4	42547.0
5	33178.0
6	23809.0
7	24296.5
8	24784.0
9	25742.5
10	26701.0
11	26666.5
12	26632.0
13	26688.5
14	26745.0
15	26212.5
16	25680.0
17	25024.5
18	24369.0
19	24957.0
20	25545.0
21	28280.5
22	31016.0
23	33639.0
24	36262.0
25	44195.5
26	52129.0
27	62708.5
28	73288.0
29	85425.0
30	97562.0
31	114536.5
32	131511.0
33	144463.5
34	157416.0
35	171492.0
36	185568.0
37	198154.5
38	210741.0
39	223871.0
40	237001.0
41	255177.0
42	273353.0
43	285535.5
44	297718.0
45	295517.5
46	293317.0
47	285468.5
48	277620.0
49	257513.5
50	237407.0
51	211622.0
52	185837.0
53	165503.0
54	145169.0
55	128909.0
56	112649.0
57	98635.5
58	84622.0
59	76181.0
60	67740.0
61	58932.5
62	50125.0
63	40679.5
64	31234.0
65	23633.5
66	16033.0
67	12512.0
68	8991.0
69	7120.0
70	5249.0
71	4428.5
72	3608.0
73	2852.5
74	2097.0
75	1676.5
76	1256.0
77	900.5
78	545.0
79	493.5
80	442.0
81	303.5
82	165.0
83	101.0
84	37.0
85	25.0
86	13.0
87	11.0
88	9.0
89	6.5
90	4.0
91	2.0
92	0.0
93	1.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3560240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.18128666716803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.52634196204762	17.97160357279124
2	6.587922946812815	3.054330607425644
3	2.5990649287920258	1.8074900754273187
4	1.5943174573561862	1.4783332006977679
5	1.084098942714058	1.2565404183314175
6	0.8619557836738799	1.1988746469460614
7	0.6952432793381094	1.1281643633231089
8	0.601375758082263	1.1152531074232344
9	0.5001409720583726	1.043452012255309
>10	6.113404065280263	32.50860708338863
>50	1.3834109674877815	22.411390173867705
>100	0.45099693986854666	14.112513731746645
>500	0.0011095691709157363	0.15784268244212718
>1k	4.93141853740232E-4	0.1548132918321312
>5k	0.0	0.0
>10k+	1.23285463435058E-4	0.6007910321017282
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21022	0.5904658112936206	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037778352021212054	0.0
2	0.0	0.0	0.0	0.12726670112127272	0.0
3	0.0	0.0	0.0	0.18906028807046715	0.0
4	0.0	0.0	0.0	0.2818911084645979	0.0
5	0.0	0.0	0.0	0.44859335325708377	0.0
6	0.0	0.0	0.0	0.7530391209581376	0.0
7	0.0	0.0	0.0	0.9256398445048648	0.0
8	0.0	0.0	0.0	1.364261959867874	0.0
9	0.0	0.0	0.0	1.5979259825180325	0.0
10	0.0	0.0	0.0	1.9457957890479294	0.0
11	0.0	0.0	0.0	2.192015145046401	0.0
12	0.0	0.0	0.0	2.4138541221940093	0.0
13	0.0	0.0	0.0	2.5412331752915533	0.0
14	5.6175988135631306E-5	0.0	0.0	2.6120710963305847	0.0
15	5.6175988135631306E-5	0.0	0.0	2.678920522211986	0.0
16	5.6175988135631306E-5	0.0	0.0	2.7851493157764646	0.0
17	5.6175988135631306E-5	0.0	0.0	2.9103936813248543	0.0
18	5.6175988135631306E-5	0.0	0.0	3.0802698694470037	0.0
19	5.6175988135631306E-5	0.0	0.0	3.1765836011055435	0.0
20	5.6175988135631306E-5	0.0	0.0	3.276604947981035	0.0
21	5.6175988135631306E-5	0.0	0.0	3.3932262993506055	0.0
22	5.6175988135631306E-5	0.0	0.0	3.528975574680359	0.0
23	5.6175988135631306E-5	0.0	0.0	3.671578320562659	0.0
24	5.6175988135631306E-5	0.0	0.0	3.788677167831382	0.0
25	5.6175988135631306E-5	0.0	0.0	3.89170393007213	0.0
26	5.6175988135631306E-5	0.0	0.0	3.999112419387457	0.0
27	5.6175988135631306E-5	0.0	0.0	4.111239691706177	0.0
28	5.6175988135631306E-5	0.0	0.0	4.228759858885918	0.0
29	5.6175988135631306E-5	0.0	0.0	4.357150079769903	0.0
30	5.6175988135631306E-5	0.0	0.0	4.5269420039098485	0.0
31	8.426398220344696E-5	0.0	0.0	4.665893310563333	0.0
32	8.426398220344696E-5	0.0	0.0	4.801895377839696	0.0
33	8.426398220344696E-5	0.0	0.0	4.939975956677078	0.0
34	8.426398220344696E-5	0.0	0.0	5.084488686155989	0.0
35	8.426398220344696E-5	0.0	0.0	5.248887715434915	0.0
36	8.426398220344696E-5	0.0	0.0	5.40671415410197	0.0
37	1.1235197627126261E-4	0.0	0.0	5.569371727748691	0.0
38	1.1235197627126261E-4	0.0	0.0	5.737225580297958	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21010	0.0	42.712044	1
ACGGGCC	205	0.0	39.707317	5
GTTCGCG	50	1.3496901E-9	39.6	1
TACGGGA	1820	0.0	38.560436	4
AGGGCGA	3800	0.0	38.500004	6
CGTAAGG	600	0.0	38.5	2
TAGGGCG	1260	0.0	38.412697	5
CGAACGG	70	0.0	37.714283	2
AAGGGAT	9620	0.0	37.642414	5
GGCGATA	1830	0.0	37.267757	8
GGGCGAT	7710	0.0	36.952007	7
AGGGATT	12570	0.0	36.754177	6
ACGTTCG	60	1.9826984E-10	36.666664	13
CGTTCGA	60	1.9826984E-10	36.666664	14
TAGGGAT	9360	0.0	36.666664	5
AGGGATC	6015	0.0	36.575226	6
GCGTAAG	255	0.0	36.23529	1
AGGGACC	4690	0.0	36.21322	6
TAAGGGA	7015	0.0	36.191017	4
TTAGGGA	7140	0.0	36.112045	4
>>END_MODULE