FastQCFastQC Report
Thu 26 May 2016
SRR1546627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546627_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1915799
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.2548284031884347No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30140.15732339352927943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT950.044.00000428
AGCGTTA207.8577996E-444.035
CGTATCG302.5286045E-644.032
GTCGACG207.8577996E-444.012
ACGCATC254.4439563E-544.043
TCCGATT207.8577996E-444.035
GCAACGC207.8577996E-444.013
TCTAGCG454.802132E-1044.01
CTCGGTA302.5286045E-644.011
CGGATGT207.8577996E-444.018
TACTCGA351.4467696E-744.033
ACGTCGA254.4439563E-544.034
TTACGCC207.8577996E-444.041
CGCACTC351.4467696E-744.019
TCGACCG207.8577996E-444.018
TCGGTAC454.802132E-1044.036
GCTAGCG1200.042.1666641
AATCGAC950.041.6842119
CGTTTTT57950.041.4184651
TGTAACG750.041.0666661
Produced by FastQC (version 0.11.5)