##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546627_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1915799
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99718603047606	31.0	31.0	33.0	30.0	34.0
2	31.36627276661069	31.0	31.0	34.0	30.0	34.0
3	31.539066467828828	31.0	31.0	34.0	30.0	34.0
4	35.310047661576185	37.0	35.0	37.0	33.0	37.0
5	35.09964876273555	37.0	35.0	37.0	32.0	37.0
6	35.179246883415225	37.0	35.0	37.0	32.0	37.0
7	35.702146728336324	37.0	35.0	37.0	35.0	37.0
8	35.74907962682933	37.0	35.0	37.0	35.0	37.0
9	37.535654314466186	39.0	37.0	39.0	35.0	39.0
10	36.770059385144265	39.0	37.0	39.0	32.0	39.0
11	36.338302713384856	38.0	35.0	39.0	32.0	39.0
12	35.71757266811393	37.0	35.0	39.0	31.0	39.0
13	35.47119713498128	37.0	35.0	39.0	30.0	39.0
14	36.38918592190517	38.0	35.0	40.0	30.0	41.0
15	36.65546124619545	38.0	35.0	40.0	31.0	41.0
16	36.75572176413079	38.0	35.0	40.0	31.0	41.0
17	36.684648546115746	38.0	35.0	40.0	31.0	41.0
18	36.646832992396384	38.0	35.0	40.0	31.0	41.0
19	36.617321023760844	38.0	35.0	40.0	31.0	41.0
20	36.45756940054776	38.0	35.0	40.0	31.0	41.0
21	36.349026176545664	38.0	35.0	40.0	31.0	41.0
22	36.25395879212798	38.0	34.0	40.0	30.0	41.0
23	36.120309072089505	38.0	34.0	40.0	30.0	41.0
24	36.072916313245805	38.0	34.0	40.0	30.0	41.0
25	35.94905624233022	37.0	34.0	40.0	30.0	41.0
26	35.84973736806418	37.0	34.0	40.0	30.0	41.0
27	35.693397898213746	37.0	34.0	40.0	29.0	41.0
28	35.69416415813976	37.0	34.0	40.0	29.0	41.0
29	35.606638274683306	37.0	34.0	40.0	29.0	41.0
30	35.56742278287023	37.0	34.0	40.0	29.0	41.0
31	35.46068611581904	37.0	34.0	40.0	29.0	41.0
32	35.24761313686874	37.0	34.0	40.0	27.0	41.0
33	35.03047866712531	37.0	34.0	40.0	27.0	41.0
34	34.97926243828293	37.0	34.0	40.0	26.0	41.0
35	34.78558293432662	37.0	33.0	40.0	25.0	41.0
36	34.55726879489967	37.0	33.0	40.0	24.0	41.0
37	34.62241863577547	37.0	33.0	40.0	24.0	41.0
38	34.61320315962165	37.0	33.0	40.0	24.0	41.0
39	34.54834666893552	37.0	33.0	40.0	24.0	41.0
40	34.509586861669725	37.0	33.0	40.0	24.0	41.0
41	34.56025658224062	37.0	33.0	40.0	24.0	41.0
42	34.31489837921411	37.0	33.0	40.0	23.0	41.0
43	34.3795507775085	37.0	33.0	40.0	23.0	41.0
44	34.20684842198999	37.0	33.0	40.0	23.0	41.0
45	34.04321434555504	36.0	33.0	40.0	23.0	41.0
46	34.13630761890992	36.0	33.0	40.0	23.0	41.0
47	34.027482528177536	36.0	33.0	40.0	23.0	41.0
48	34.08804055122693	36.0	33.0	40.0	23.0	41.0
49	34.0231407365804	36.0	33.0	40.0	23.0	41.0
50	33.51018765538556	35.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	16.0
15	39.0
16	149.0
17	464.0
18	1057.0
19	2095.0
20	3743.0
21	5971.0
22	8925.0
23	12866.0
24	18051.0
25	24777.0
26	32155.0
27	37799.0
28	43267.0
29	49980.0
30	59602.0
31	72120.0
32	88894.0
33	112034.0
34	169371.0
35	229794.0
36	147913.0
37	178036.0
38	248991.0
39	367686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.61641748429767	24.886431196592127	29.975795999475935	12.521355319634262
2	28.12513212502982	27.86680648648423	30.367068779136012	13.640992609349937
3	27.205620213811578	26.600494101938672	32.93711918630295	13.25676649794681
4	23.557794946129526	30.034779222663754	32.07293667028744	14.334489160919281
5	21.987692863395377	33.991874930512026	30.67143265029369	13.348999555798912
6	21.073191916270968	42.681147656930605	26.520997244491724	9.724663182306703
7	90.34475954940994	3.5775151777404623	5.126738243416976	0.9509870294326284
8	91.00505846385764	2.823678266874552	4.567545969070868	1.6037173001969411
9	85.55156360348867	6.069060480770686	6.384960008852703	1.9944159068879357
10	41.22102579654755	38.656403933815604	11.447390879732163	8.675179389904681
11	33.20572774074942	24.185679186595255	29.644550393856555	12.964042678798767
12	32.276820271855236	24.7440884978017	28.155876477647183	14.823214752695874
13	20.436590686183674	37.098568273602815	28.83507090253205	13.629770137681458
14	16.816586708730927	39.93007617187398	28.29091152046744	14.962425598927654
15	14.782657262061417	26.816435335857257	43.48582497433186	14.915082427749466
16	16.67346104680084	24.634317065621183	42.13630970681162	16.555912180766356
17	17.202587536583952	23.705409596727005	31.060878515961228	28.031124350727815
18	19.369411926825308	26.043911704724763	35.93889546867913	18.6477808997708
19	25.0755950911343	25.93403587745896	29.593240209437422	19.39712882196932
20	26.528096110291322	27.317322955069923	28.670961828459042	17.483619106179717
21	21.383610702375353	30.48487863288372	29.92704349464636	18.204467170094567
22	21.26366074937924	27.158329240176034	28.1027393792355	23.475270631209224
23	17.762719366697656	32.31915247893959	28.143975437924336	21.774152716438415
24	16.65242543711527	27.814191363498992	39.00388297519729	16.529500224188446
25	15.787459957960099	28.409191152098938	37.25161146863528	18.55173742130568
26	16.02882139514636	35.059262480040964	30.302709209055855	18.609206915756822
27	16.331619340024712	36.35757195822735	29.867329505861523	17.443479195886415
28	15.302753576967104	31.470942410973173	36.83022070686956	16.39608330519016
29	15.039469171870326	27.058684131268468	37.163241028938835	20.738605667922368
30	17.442435245033533	33.262466469603545	31.757454722546573	17.53764356281635
31	24.734536347497833	29.5497596564149	28.005286567118993	17.710417428968277
32	26.067870376798403	28.22655195038728	29.12121783130694	16.584359841507382
33	24.888310308127316	29.54814153259293	27.400891220843103	18.16265693843665
34	18.472136168773446	29.37672480254975	29.24884082307173	22.902298205605078
35	18.515616721795972	28.934350628641102	31.54527171169836	21.004760937864567
36	26.427146062817652	27.960709865700945	27.79769694002346	17.814447131457946
37	18.954859043145966	34.29503825818888	29.299576834521783	17.45052586414337
38	18.267939381949777	34.84259048052536	26.845039589226218	20.044430548298646
39	18.926254789777005	34.09313816324155	28.61469287748871	18.36591416949273
40	22.720807349831585	28.604305566502543	27.686463976648906	20.988423107016967
41	18.070893658468346	26.94170943820307	28.89802113896082	26.089375764367766
42	19.214541817800303	27.52047579104071	28.252911709422545	25.012070681736446
43	19.21094018735786	27.150082028438266	29.690014453499558	23.948963330704316
44	18.008413199923375	30.46253808463205	30.93424727750667	20.594801437937903
45	16.43298696783953	36.076122808290435	27.5170307532262	19.97385947064384
46	19.694759210125905	34.21251394326858	27.588332596478022	18.504394250127493
47	19.19679465330131	29.949801623239182	28.63520651174784	22.21819721171167
48	19.83757168680013	28.193563103436215	32.85396850087092	19.11489670889274
49	20.333239551748385	27.12283491117805	32.88079803778998	19.66312749928359
50	19.07825403395659	33.4279326797853	28.925007268507812	18.568806017750298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1852.0
1	2238.0
2	2624.0
3	6590.5
4	10557.0
5	8808.0
6	7059.0
7	7786.5
8	8514.0
9	9395.5
10	10277.0
11	10549.5
12	10822.0
13	11275.0
14	11728.0
15	11384.5
16	11041.0
17	11267.0
18	11493.0
19	11989.0
20	12485.0
21	13701.5
22	14918.0
23	15719.5
24	16521.0
25	20465.5
26	24410.0
27	28905.0
28	33400.0
29	39446.0
30	45492.0
31	53795.0
32	62098.0
33	69796.5
34	77495.0
35	84477.5
36	91460.0
37	96214.5
38	100969.0
39	115906.0
40	130843.0
41	148688.5
42	166534.0
43	184671.0
44	202808.0
45	203366.5
46	203925.0
47	186273.0
48	168621.0
49	153367.5
50	138114.0
51	118816.5
52	99519.0
53	87308.0
54	75097.0
55	64393.5
56	53690.0
57	43797.0
58	33904.0
59	29982.0
60	26060.0
61	23286.5
62	20513.0
63	16156.0
64	11799.0
65	9255.0
66	6711.0
67	5627.5
68	4544.0
69	3936.0
70	3328.0
71	2538.0
72	1748.0
73	1432.5
74	1117.0
75	966.0
76	815.0
77	622.5
78	430.0
79	375.5
80	321.0
81	205.5
82	90.0
83	64.5
84	39.0
85	24.0
86	9.0
87	6.5
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1915799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.75438337097487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.47512099097176	16.9096589624319
2	6.425446053225395	2.667123414363145
3	2.2338776272492864	1.3908825803932616
4	1.1351591104288916	0.9423810945958406
5	0.7914202069427154	0.821271919121269
6	0.572643301656953	0.7130915170445526
7	0.4551730838427392	0.6612785677554769
8	0.3937704599015205	0.6537970467968995
9	0.3036366664703337	0.5671612603269124
>10	3.8549061342905517	18.842263361136744
>50	1.3452717117658228	20.083543527856364
>100	1.0072302951366867	34.550004959480994
>500	0.005329260818550348	0.646410272800913
>1k	0.001015097298771495	0.5511315158957675
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4882	0.2548284031884347	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3014	0.15732339352927943	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04713438100761092	0.0
2	0.0	0.0	0.0	0.15147726875314163	0.0
3	0.0	0.0	0.0	0.21547145603479279	0.0
4	0.0	0.0	0.0	0.36402566239986556	0.0
5	0.0	0.0	0.0	0.5683790418514677	0.0
6	0.0	0.0	0.0	0.9051575869911196	0.0
7	0.0	0.0	0.0	1.0570524360854139	0.0
8	0.0	0.0	0.0	1.5227067140133177	0.0
9	0.0	0.0	0.0	1.7405270594670945	0.0
10	0.0	0.0	0.0	2.0960445224159736	0.0
11	0.0	0.0	0.0	2.3925787621770342	0.0
12	0.0	0.0	0.0	2.639681929054144	0.0
13	0.0	0.0	0.0	2.7718983045716175	0.0
14	0.0	0.0	0.0	2.8317688859843857	0.0
15	0.0	0.0	0.0	2.8922136403662386	0.0
16	0.0	0.0	0.0	3.0073092218964517	0.0
17	0.0	0.0	0.0	3.1456327099032833	0.0
18	0.0	0.0	0.0	3.3243048983739945	0.0
19	0.0	0.0	0.0	3.4139802766365364	0.0
20	0.0	0.0	0.0	3.510389137900166	0.0
21	0.0	0.0	0.0	3.6322182024314658	0.0
22	0.0	0.0	0.0	3.7680362083913814	0.0
23	0.0	0.0	0.0	3.9222277493620155	0.0
24	0.0	0.0	0.0	4.0315816012013785	0.0
25	0.0	0.0	0.0	4.128042660007652	0.0
26	0.0	0.0	0.0	4.23055863376064	0.0
27	0.0	0.0	0.0	4.333179002598916	0.0
28	0.0	0.0	0.0	4.43960979205021	0.0
29	5.21975426440874E-5	0.0	0.0	4.54943342177337	0.0
30	5.21975426440874E-5	0.0	0.0	4.707487580899667	0.0
31	5.21975426440874E-5	0.0	0.0	4.8394951662465635	0.0
32	5.21975426440874E-5	0.0	0.0	4.958818748730947	0.0
33	5.21975426440874E-5	0.0	0.0	5.076732997563941	0.0
34	5.21975426440874E-5	0.0	0.0	5.206496088577142	0.0
35	5.21975426440874E-5	0.0	0.0	5.364759037874015	0.0
36	5.21975426440874E-5	0.0	0.0	5.510494576936307	0.0
37	5.21975426440874E-5	0.0	0.0	5.65586473320009	0.0
38	5.21975426440874E-5	0.0	0.0	5.809429903658995	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	95	0.0	44.000004	28
AGCGTTA	20	7.8577996E-4	44.0	35
CGTATCG	30	2.5286045E-6	44.0	32
GTCGACG	20	7.8577996E-4	44.0	12
ACGCATC	25	4.4439563E-5	44.0	43
TCCGATT	20	7.8577996E-4	44.0	35
GCAACGC	20	7.8577996E-4	44.0	13
TCTAGCG	45	4.802132E-10	44.0	1
CTCGGTA	30	2.5286045E-6	44.0	11
CGGATGT	20	7.8577996E-4	44.0	18
TACTCGA	35	1.4467696E-7	44.0	33
ACGTCGA	25	4.4439563E-5	44.0	34
TTACGCC	20	7.8577996E-4	44.0	41
CGCACTC	35	1.4467696E-7	44.0	19
TCGACCG	20	7.8577996E-4	44.0	18
TCGGTAC	45	4.802132E-10	44.0	36
GCTAGCG	120	0.0	42.166664	1
AATCGAC	95	0.0	41.68421	19
CGTTTTT	5795	0.0	41.418465	1
TGTAACG	75	0.0	41.066666	1
>>END_MODULE