FastQCFastQC Report
Thu 26 May 2016
SRR1546626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546626_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3678641
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195010.5301142459946485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT145850.041.1642071
TATCGCT404.1278327E-738.50000433
AGGGTAC15450.038.0194176
TACGGGA9550.037.549744
AGGGCGA47950.037.209596
TAGGGTA18700.037.176475
GGGCGAT90600.036.9580577
GTAGGGT19850.036.795974
TAGGGCG20450.036.6845975
GGCGATA18750.036.4906658
ATAGGGC20700.036.3478244
AGGGATT36450.036.274356
TACGTAG2250.036.177781
ACATACG7300.036.16438317
AAGGGAT37350.035.8714875
GCGATAC2700.035.8518529
TAAGGGC23400.035.7264984
TAGCGGG21650.035.667443
GCGATGT9200.035.6304369
TAAGGGA36500.035.621924
Produced by FastQC (version 0.11.5)