##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546626_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3678641
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93540060038476	31.0	31.0	33.0	30.0	34.0
2	31.311312791870694	31.0	31.0	34.0	30.0	34.0
3	31.478651762974426	31.0	31.0	34.0	30.0	34.0
4	35.2719384685812	37.0	35.0	37.0	33.0	37.0
5	35.06703807194015	37.0	35.0	37.0	32.0	37.0
6	35.13459100792929	37.0	35.0	37.0	32.0	37.0
7	35.61771670570735	36.0	35.0	37.0	35.0	37.0
8	35.64596545300289	37.0	35.0	37.0	35.0	37.0
9	37.405976011249805	39.0	37.0	39.0	35.0	39.0
10	36.62482177521536	39.0	35.0	39.0	32.0	39.0
11	36.29719915588392	38.0	35.0	39.0	32.0	39.0
12	35.80707304681267	37.0	35.0	39.0	30.0	39.0
13	35.66447419033279	37.0	35.0	39.0	30.0	39.0
14	36.54258298105197	38.0	35.0	40.0	30.0	41.0
15	36.72398665702905	38.0	35.0	40.0	31.0	41.0
16	36.76443773665329	38.0	35.0	40.0	31.0	41.0
17	36.66798499772062	38.0	35.0	40.0	31.0	41.0
18	36.60020262917746	38.0	35.0	40.0	31.0	41.0
19	36.54478270643969	38.0	35.0	40.0	30.0	41.0
20	36.40260057994243	38.0	35.0	40.0	30.0	41.0
21	36.29311993206187	38.0	35.0	40.0	30.0	41.0
22	36.22860779293223	38.0	34.0	40.0	30.0	41.0
23	36.00571624140545	38.0	34.0	40.0	30.0	41.0
24	35.94968141767571	38.0	34.0	40.0	30.0	41.0
25	35.76671629550152	38.0	34.0	40.0	29.0	41.0
26	35.63436687624588	37.0	34.0	40.0	29.0	41.0
27	35.45825183811087	37.0	34.0	40.0	28.0	41.0
28	35.38504001885479	37.0	34.0	40.0	27.0	41.0
29	35.24468519760423	37.0	34.0	40.0	27.0	41.0
30	35.207066142088884	37.0	34.0	40.0	27.0	41.0
31	35.13680160689776	37.0	34.0	40.0	27.0	41.0
32	34.961860371805784	37.0	33.0	40.0	26.0	41.0
33	34.758742698730316	37.0	33.0	40.0	25.0	41.0
34	34.60326000824761	37.0	33.0	40.0	24.0	41.0
35	34.38749554522988	37.0	33.0	40.0	24.0	41.0
36	34.17986669533668	37.0	33.0	40.0	23.0	41.0
37	34.24664434501763	37.0	33.0	40.0	23.0	41.0
38	34.16238551138858	37.0	33.0	40.0	23.0	41.0
39	34.06040545951616	37.0	33.0	40.0	23.0	41.0
40	34.00276922918002	36.0	33.0	40.0	23.0	41.0
41	34.001161026585635	36.0	33.0	40.0	23.0	41.0
42	33.76481341886854	36.0	32.0	40.0	22.0	41.0
43	33.799495520220646	36.0	33.0	40.0	23.0	41.0
44	33.591337398783956	36.0	32.0	40.0	22.0	41.0
45	33.33520096144201	35.0	32.0	40.0	21.0	41.0
46	33.43193695715347	35.0	32.0	40.0	22.0	41.0
47	33.279841387077454	35.0	32.0	40.0	21.0	41.0
48	33.33831406761356	35.0	32.0	39.0	22.0	41.0
49	33.238734358693875	35.0	32.0	39.0	21.0	41.0
50	32.75777603740077	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	14.0
14	38.0
15	134.0
16	412.0
17	1150.0
18	2557.0
19	4967.0
20	8576.0
21	13331.0
22	19984.0
23	28847.0
24	40181.0
25	53610.0
26	69326.0
27	82179.0
28	93441.0
29	106122.0
30	124705.0
31	147678.0
32	178040.0
33	220788.0
34	314145.0
35	376398.0
36	321036.0
37	386516.0
38	493740.0
39	590718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.63609006695679	23.72397850184348	28.32575943126823	14.314171999931496
2	32.15698406014613	25.714713667357046	28.594499979747955	13.53380229274887
3	29.02194043941771	25.75717500022427	31.5883773382616	13.632507222096422
4	25.432571430590805	28.512295709203478	30.830053816069576	15.225079044136136
5	22.57105273387645	33.00074674315868	29.992081314811635	14.436119208153228
6	21.40216998614434	41.18417643907084	27.14741666827505	10.266236906509768
7	88.43176053330563	3.5372030051315146	6.66425454400144	1.3667819175614038
8	88.67345304964523	3.0601790171968397	6.482747297167622	1.7836206359903017
9	82.82411901569084	5.137739725077821	8.889451294649302	3.148689964582029
10	41.682947588525224	26.494077568319383	17.411348375663728	14.411626467491665
11	34.43162841929941	25.558868071116482	24.282065034342846	15.727438475241264
12	31.21829501710007	22.09449087312407	28.60482988146981	18.08238422830605
13	24.090608461113764	27.738368598621065	29.730354225922017	18.44066871434315
14	20.896303825244157	29.488879181197618	30.853214543088058	18.76160245047016
15	20.198600515788304	23.681707456639558	37.272867887896645	18.846824139675494
16	22.600628873543247	21.51041648260866	36.351848413585344	19.53710623026275
17	22.96818308717812	21.529119041515603	29.85817316775407	25.644524703552207
18	24.390556186374262	22.731383682180457	32.04688905495263	20.831171076492648
19	27.229539386963825	24.802719265076423	27.43208701256796	20.535654335391794
20	29.10433499762548	24.324662286969563	26.891724416707145	19.67927829869781
21	26.35070940600075	25.994789923778917	28.867426856820224	18.787073813400113
22	25.248101132999935	23.677031816912823	27.881057162142213	23.193809887945033
23	23.41671829352198	26.525257561148262	27.93257075099201	22.125453394337747
24	23.109594004959984	24.249417108111395	32.663502635891895	19.977486251036726
25	23.223250107852326	24.133559105115175	30.908914460530397	21.734276326502098
26	21.59966139669514	27.5017051133829	28.38382435252584	22.51480913739612
27	21.369603611768582	28.0209729625696	28.773832510429802	21.83559091523201
28	20.740240757388396	27.504151669053872	31.24689253449847	20.50871503905926
29	21.51414068401891	25.323210392098606	30.57305673481049	22.589592189071997
30	22.683295271270016	26.928966430809638	30.113321740283983	20.274416557636364
31	25.851258657748883	26.211636308082255	27.26025725261041	20.67684778155846
32	27.11237655427643	25.38717423091843	27.212114473796166	20.288334741008978
33	26.608059878634528	26.021783588015246	26.06351095418118	21.306645579169047
34	22.98555363244198	27.50798460627172	27.487080147260905	22.019381614025395
35	22.907671610249547	26.943727316691135	28.57362270468904	21.574978368370274
36	26.880660548284002	27.25933299824582	25.136130435125363	20.723876018344818
37	23.13202076527718	30.533803108267428	25.649336263038442	20.68483986341695
38	23.579631717256454	28.896676789064223	25.66453209215033	21.859159401528988
39	23.029129507337085	28.58183225816273	25.680570623771114	22.708467610729073
40	25.228800527151197	25.707591471959347	27.00622322210838	22.057384778781078
41	21.46923279548072	25.411503867868596	27.736030778757698	25.38323255789298
42	22.36423722782408	26.057149909436667	26.738379744041346	24.84023311869791
43	22.153480048746264	25.25272240482287	28.00142770115377	24.5923698452771
44	22.13681628623179	26.809384226403175	28.52333239367473	22.530467093690305
45	21.32088996996445	28.891430286347592	26.623581915169215	23.164097828518738
46	22.674514854806436	28.125332153912275	26.807046406539808	22.393106584741485
47	21.85464686551365	27.427547292600718	27.88491728331196	22.832888558573668
48	22.633548639293696	26.780868260860462	28.50805501270714	22.077528087138703
49	22.75802395504209	26.061961468922902	29.329526855162	21.85048772087301
50	21.988935587897814	28.261061625747118	27.55373519731879	22.196267589036278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2756.0
1	4153.0
2	5550.0
3	20463.5
4	35377.0
5	25988.0
6	16599.0
7	17143.5
8	17688.0
9	18433.0
10	19178.0
11	19665.5
12	20153.0
13	20126.0
14	20099.0
15	19827.0
16	19555.0
17	18814.5
18	18074.0
19	18121.5
20	18169.0
21	19698.0
22	21227.0
23	22061.5
24	22896.0
25	27404.5
26	31913.0
27	36698.0
28	41483.0
29	45779.0
30	50075.0
31	62340.5
32	74606.0
33	81905.0
34	89204.0
35	101039.0
36	112874.0
37	121262.5
38	129651.0
39	141898.5
40	154146.0
41	184231.5
42	214317.0
43	226236.5
44	238156.0
45	245440.0
46	252724.0
47	257001.5
48	261279.0
49	259463.0
50	257647.0
51	240397.5
52	223148.0
53	210191.5
54	197235.0
55	195335.0
56	193435.0
57	190625.5
58	187816.0
59	182904.0
60	177992.0
61	167398.0
62	156804.0
63	142254.0
64	127704.0
65	107654.0
66	87604.0
67	74809.5
68	62015.0
69	52539.0
70	43063.0
71	37311.5
72	31560.0
73	25729.0
74	19898.0
75	15871.0
76	11844.0
77	9310.0
78	6776.0
79	5019.5
80	3263.0
81	2628.0
82	1993.0
83	1305.0
84	617.0
85	469.0
86	321.0
87	195.5
88	70.0
89	60.5
90	51.0
91	37.0
92	23.0
93	17.5
94	12.0
95	6.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3678641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.714360367908917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59913453456605	16.48845157722665
2	6.266407153403483	2.596092319752841
3	2.2850073333979015	1.419973960419562
4	1.2753528572822466	1.0567247472794705
5	0.9039228667227559	0.9362092003044236
6	0.6902927405345088	0.8579383552069959
7	0.5645114141059862	0.8185445004512482
8	0.4681611912654226	0.7758127700913198
9	0.40282382571047853	0.7509814101491927
>10	5.176417302846001	25.386806772009706
>50	1.5738242507844529	23.187865332963774
>100	0.7897457344298251	24.30649576439447
>500	0.0029325299673678613	0.4137043476378076
>1k	0.001332968166985388	0.4659454626168316
>5k	0.0	0.0
>10k+	1.332968166985388E-4	0.5384534794957531
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19501	0.5301142459946485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022453944269092852	0.0
2	0.0	0.0	0.0	0.07317919851379898	0.0
3	0.0	0.0	0.0	0.11479782887212968	0.0
4	0.0	0.0	0.0	0.1946914635051368	0.0
5	0.0	0.0	0.0	0.31568723341038174	0.0
6	0.0	0.0	0.0	0.6010915444045777	0.0
7	0.0	0.0	0.0	0.7559857023286589	0.0
8	0.0	0.0	0.0	1.1870144436491628	0.0
9	0.0	0.0	0.0	1.4246837351076118	0.0
10	0.0	0.0	0.0	1.761683186807302	0.0
11	0.0	0.0	0.0	1.9930186174731375	0.0
12	0.0	0.0	0.0	2.193282791117698	0.0
13	0.0	0.0	0.0	2.3022088863795083	0.0
14	0.0	0.0	0.0	2.364351400422058	0.0
15	0.0	0.0	0.0	2.4144514237730728	0.0
16	0.0	0.0	0.0	2.4936926435604887	0.0
17	0.0	0.0	0.0	2.5794036439000165	0.0
18	0.0	0.0	0.0	2.708255575904254	0.0
19	0.0	0.0	0.0	2.7729805653772686	0.0
20	0.0	0.0	0.0	2.8455617169492755	0.0
21	0.0	0.0	0.0	2.9300766234052196	0.0
22	0.0	0.0	0.0	3.0222030363930594	0.0
23	0.0	0.0	0.0	3.1281388969459103	0.0
24	0.0	0.0	0.0	3.2147741516500252	0.0
25	0.0	0.0	0.0	3.291976575044969	0.0
26	0.0	0.0	0.0	3.3698857811893035	0.0
27	0.0	0.0	0.0	3.45421039998195	0.0
28	0.0	0.0	0.0	3.5363331186707265	0.0
29	0.0	0.0	0.0	3.633896322038492	0.0
30	0.0	0.0	0.0	3.7807168462483838	0.0
31	0.0	0.0	0.0	3.8885012155304093	0.0
32	0.0	0.0	0.0	4.000906856635371	0.0
33	0.0	0.0	0.0	4.108908697532594	0.0
34	0.0	0.0	0.0	4.225228827711103	0.0
35	0.0	0.0	0.0	4.366096066454976	0.0
36	0.0	0.0	0.0	4.483258899142373	0.0
37	0.0	0.0	0.0	4.603738173961525	0.0
38	0.0	0.0	0.0	4.752053815525897	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14585	0.0	41.164207	1
TATCGCT	40	4.1278327E-7	38.500004	33
AGGGTAC	1545	0.0	38.019417	6
TACGGGA	955	0.0	37.54974	4
AGGGCGA	4795	0.0	37.20959	6
TAGGGTA	1870	0.0	37.17647	5
GGGCGAT	9060	0.0	36.958057	7
GTAGGGT	1985	0.0	36.79597	4
TAGGGCG	2045	0.0	36.684597	5
GGCGATA	1875	0.0	36.490665	8
ATAGGGC	2070	0.0	36.347824	4
AGGGATT	3645	0.0	36.27435	6
TACGTAG	225	0.0	36.17778	1
ACATACG	730	0.0	36.164383	17
AAGGGAT	3735	0.0	35.871487	5
GCGATAC	270	0.0	35.851852	9
TAAGGGC	2340	0.0	35.726498	4
TAGCGGG	2165	0.0	35.66744	3
GCGATGT	920	0.0	35.630436	9
TAAGGGA	3650	0.0	35.62192	4
>>END_MODULE