FastQCFastQC Report
Thu 26 May 2016
SRR1546625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546625_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1633306
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63850.3909249093556259No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22800.13959417280044278No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA650.044.00000431
ATCGCTA207.8574556E-444.00000414
TCGAATA207.8574556E-444.00000439
TGCGTAA207.8574556E-444.00000430
ATTCGAC254.443666E-544.018
CGTAACA254.443666E-544.033
GTTACGG1800.041.5555572
CGTTTTT53300.039.8311421
ATTACCG501.3478711E-939.6000021
TAACGGG4450.038.56183
TCGATTG750.038.133331
GCATATC4800.037.12500428
GGGCGAT34000.036.9470567
TAGGGCG6500.036.8923075
ACGTCCA301.3013349E-436.66666817
GTACGGA301.3013349E-436.6666683
ATAGGGC8850.036.542374
AGGGACC15750.036.317466
GCGATAA1950.036.1025669
AGGGCGA17750.035.943666
Produced by FastQC (version 0.11.5)