##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546625_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1633306
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.957413981213563	31.0	31.0	33.0	30.0	34.0
2	31.33138064759451	31.0	31.0	34.0	30.0	34.0
3	31.502393917612498	31.0	31.0	34.0	30.0	34.0
4	35.26603404383502	37.0	35.0	37.0	33.0	37.0
5	35.063754128130306	37.0	35.0	37.0	32.0	37.0
6	35.131392402893276	37.0	35.0	37.0	32.0	37.0
7	35.63815414870208	37.0	35.0	37.0	35.0	37.0
8	35.67330494102146	37.0	35.0	37.0	35.0	37.0
9	37.43355990855357	39.0	37.0	39.0	35.0	39.0
10	36.66872772156595	39.0	35.0	39.0	32.0	39.0
11	36.26714896045199	38.0	35.0	39.0	31.0	39.0
12	35.568976664507446	37.0	35.0	39.0	30.0	39.0
13	35.27247558020359	37.0	35.0	39.0	30.0	39.0
14	36.153278075265746	38.0	35.0	40.0	30.0	41.0
15	36.43270826164846	38.0	35.0	40.0	30.0	41.0
16	36.54467074755129	38.0	35.0	40.0	31.0	41.0
17	36.46043301132794	38.0	35.0	40.0	31.0	41.0
18	36.405489234717805	38.0	35.0	40.0	31.0	41.0
19	36.355615542953984	38.0	35.0	40.0	30.0	41.0
20	36.20244767361413	38.0	34.0	40.0	30.0	41.0
21	36.061734298410705	38.0	34.0	40.0	30.0	41.0
22	35.98476219397957	38.0	34.0	40.0	30.0	41.0
23	35.81287156234043	37.0	34.0	40.0	30.0	41.0
24	35.75095603640714	37.0	34.0	40.0	30.0	41.0
25	35.582479339450174	37.0	34.0	40.0	29.0	41.0
26	35.4569468305388	37.0	34.0	40.0	29.0	41.0
27	35.25426588771486	37.0	34.0	40.0	28.0	41.0
28	35.21868712904991	36.0	34.0	40.0	27.0	41.0
29	35.11430130055238	36.0	34.0	40.0	27.0	41.0
30	35.085821640280514	36.0	34.0	40.0	27.0	41.0
31	34.99694607134242	36.0	34.0	40.0	27.0	41.0
32	34.81600508416671	36.0	33.0	40.0	26.0	41.0
33	34.60648219011012	36.0	33.0	40.0	25.0	41.0
34	34.502210853324485	36.0	33.0	40.0	25.0	41.0
35	34.289161369639245	36.0	33.0	40.0	24.0	41.0
36	34.0567033978936	36.0	33.0	40.0	23.0	41.0
37	34.103514589427824	36.0	33.0	40.0	23.0	41.0
38	34.04763222568214	36.0	33.0	40.0	23.0	41.0
39	33.95539721276968	36.0	33.0	40.0	23.0	41.0
40	33.8926484075856	36.0	33.0	40.0	23.0	41.0
41	33.914976740427086	36.0	33.0	40.0	23.0	41.0
42	33.695040610883694	35.0	33.0	40.0	23.0	41.0
43	33.72951241224853	35.0	33.0	40.0	23.0	41.0
44	33.513079606638314	35.0	32.0	40.0	22.0	41.0
45	33.32453685959643	35.0	32.0	40.0	22.0	41.0
46	33.413724678657886	35.0	32.0	39.0	23.0	41.0
47	33.260729465268604	35.0	32.0	39.0	22.0	41.0
48	33.31383525193687	35.0	32.0	39.0	22.0	41.0
49	33.227330334915806	35.0	32.0	39.0	22.0	41.0
50	32.72696420633978	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	6.0
14	20.0
15	78.0
16	203.0
17	523.0
18	1169.0
19	2350.0
20	3866.0
21	6058.0
22	9264.0
23	12800.0
24	17631.0
25	23966.0
26	30847.0
27	36423.0
28	41455.0
29	47800.0
30	56455.0
31	67024.0
32	81259.0
33	101792.0
34	152466.0
35	196654.0
36	131241.0
37	155708.0
38	202592.0
39	253654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.5469936435671	24.877212230898557	29.443900897933396	13.131893227600951
2	31.182399378928384	25.782860039698623	29.253122195106123	13.781618386266873
3	29.599413704474237	25.71018535412225	30.92996658311425	13.760434358289261
4	25.489161247188218	28.7896450512029	30.933333986405486	14.787859715203396
5	22.577337008496876	33.19892292075092	30.028298432749285	14.195441638002922
6	21.578871319887394	41.838394030267445	26.800366863282203	9.78236778656296
7	88.28547743043863	4.144416294313497	6.146062036140196	1.4240442391076749
8	88.8713443775998	3.4330370426607137	5.841036523468352	1.854582056271146
9	83.37813000135921	5.359252950763667	8.336833391905742	2.9257836559713857
10	47.0924003218013	25.069399120556714	14.886310342336342	12.951890215305644
11	38.58517632335888	23.645538557992197	23.197612694743057	14.571672423905868
12	35.49720627977856	21.802466898425646	26.276705038737386	16.423621783058408
13	22.66115473769153	33.133595296900886	27.89030347038461	16.314946495022976
14	18.671026739631152	35.51655354232459	28.970260318642065	16.842159399402192
15	17.230757739211146	24.05832097598368	42.04160151251511	16.669319772290066
16	19.319711064552507	20.90826826081579	40.834479270877594	18.937541403754103
17	20.290380369630675	21.004882122517152	29.218591004992327	29.486146502859846
18	22.15598301849133	23.520393606586886	33.48845837828306	20.83516499663872
19	28.028305779811006	24.361754625281485	27.30762024997153	20.302319344935977
20	29.91288833813137	24.101913542226626	26.756774297039254	19.228423822602743
21	24.14997557101976	27.52111361863607	29.03332259845981	19.29558821188436
22	24.01307532085231	24.55314558325262	26.686854759610263	24.746924336284813
23	21.79891581859125	29.246203711980485	26.06357902315916	22.8913014462691
24	20.912676497851596	24.732964919004765	35.85855926568567	18.495799317457966
25	20.655774239487272	24.260181496914846	34.59088499032025	20.493159273277634
26	19.49077515174744	31.018498676916632	28.243819590450286	21.24690658088564
27	19.751473392003703	31.980596409980738	28.35684189000714	19.91108830800842
28	18.869030053155992	28.04440809009457	34.510924468531925	18.575637388217515
29	19.081605039104733	25.026051456371313	33.93497605470132	21.95736744982263
30	20.66440703701572	29.74047728961995	30.225873167673416	19.369242505690913
31	27.33933506642356	26.933287455014554	26.447218096302837	19.28015938225905
32	28.829135507981967	25.416609012640617	26.875306892890862	18.878948586486548
33	28.70062315328542	26.165703181155276	25.73718580596655	19.396487859592753
34	22.053675183952056	26.61381272094758	27.627646013667984	23.70486608143238
35	21.337214214605225	26.34576741896497	30.576940267163653	21.74007809926615
36	28.79631863227099	25.342709816776527	26.435462797540694	19.42550875341179
37	22.784095570578934	31.264808921292154	27.461541193138334	18.489554314990578
38	22.073451025098787	31.2849521155252	25.193870591303774	21.44772626807224
39	21.55395253553223	31.434036243055495	26.476055313578716	20.535955907833557
40	25.176237643160558	26.102702126851916	26.656058325874028	22.065001904113497
41	19.72949343233907	25.18603372546234	27.9319368201672	27.152536022031388
42	21.498176091926435	25.597469182137335	26.298256419801312	26.606098306134918
43	21.470134806337576	26.014292484078304	27.64331974535084	24.87225296423328
44	20.638631095459147	27.52723617007468	29.7654572994895	22.06867543497667
45	19.41552899456685	32.10555768484289	26.173540046996706	22.30537327359356
46	22.167738317253473	30.56579722354537	27.153699306804725	20.112765152396427
47	21.68062812479719	27.45382677832568	27.66248333135371	23.203061765523422
48	22.182922244821242	25.76810469073156	30.76906593130742	21.27990713313978
49	21.986633245699213	25.41764984638518	31.511731420811532	21.08398548710407
50	20.805837975247748	30.885027055554808	27.533481172542075	20.775653796655373
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1949.0
1	2576.5
2	3204.0
3	7614.5
4	12025.0
5	9264.0
6	6503.0
7	6885.0
8	7267.0
9	7892.0
10	8517.0
11	8802.5
12	9088.0
13	9261.0
14	9434.0
15	9207.5
16	8981.0
17	8776.0
18	8571.0
19	8715.5
20	8860.0
21	8906.5
22	8953.0
23	9881.0
24	10809.0
25	12826.5
26	14844.0
27	16815.5
28	18787.0
29	21932.0
30	25077.0
31	28524.0
32	31971.0
33	35433.5
34	38896.0
35	44654.5
36	50413.0
37	53880.0
38	57347.0
39	67923.0
40	78499.0
41	95207.5
42	111916.0
43	127042.0
44	142168.0
45	140029.0
46	137890.0
47	136718.5
48	135547.0
49	127785.0
50	120023.0
51	107883.5
52	95744.0
53	88392.5
54	81041.0
55	76281.5
56	71522.0
57	68336.5
58	65151.0
59	61598.5
60	58046.0
61	55232.5
62	52419.0
63	47613.5
64	42808.0
65	37202.0
66	31596.0
67	27466.0
68	23336.0
69	19866.0
70	16396.0
71	13704.0
72	11012.0
73	9164.5
74	7317.0
75	5778.5
76	4240.0
77	3574.0
78	2908.0
79	2029.5
80	1151.0
81	843.0
82	535.0
83	434.0
84	333.0
85	250.5
86	168.0
87	100.5
88	33.0
89	18.0
90	3.0
91	3.0
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1633306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.182237844112176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30285626688828	17.8129705727367
2	5.89232405854886	2.6140986744263035
3	2.095260005877793	1.3943266738691127
4	1.2749720689810304	1.1312693471494806
5	0.9000769739432214	0.9982860757008648
6	0.6788137410250187	0.9034564713161118
7	0.534891409186961	0.8305561921550247
8	0.4499497135387893	0.7984713250886053
9	0.4073825036942176	0.8132990031427537
>10	5.3090961233569445	28.019811057495993
>50	1.5321464978973003	23.65375442857454
>100	0.6205564739126774	20.279972399479597
>500	8.370815744333513E-4	0.1366011069062097
>1k	5.580543829555675E-4	0.21793044740224934
>5k	2.7902719147778377E-4	0.3951962245564789
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6385	0.3909249093556259	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2280	0.13959417280044278	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03153113990887194	0.0
2	0.0	0.0	0.0	0.11100185758210647	0.0
3	0.0	0.0	0.0	0.16506398678508497	0.0
4	0.0	0.0	0.0	0.2772291291405285	0.0
5	0.0	0.0	0.0	0.4398440953501671	0.0
6	0.0	0.0	0.0	0.7396042137848021	0.0
7	0.0	0.0	0.0	0.8879536351424656	0.0
8	0.0	0.0	0.0	1.320205766708749	0.0
9	0.0	0.0	0.0	1.5316786933985427	0.0
10	0.0	0.0	0.0	1.8813988315722834	0.0
11	0.0	0.0	0.0	2.1595463434286044	0.0
12	0.0	0.0	0.0	2.3881624141465223	0.0
13	0.0	0.0	0.0	2.508531775429711	0.0
14	0.0	0.0	0.0	2.5707368980460488	0.0
15	0.0	0.0	0.0	2.6257174099648197	0.0
16	0.0	0.0	0.0	2.732433481539895	0.0
17	0.0	0.0	0.0	2.844659849409725	0.0
18	0.0	0.0	0.0	2.999376724263549	0.0
19	0.0	0.0	0.0	3.0741943028434355	0.0
20	0.0	0.0	0.0	3.155746688005799	0.0
21	0.0	0.0	0.0	3.258789228717705	0.0
22	0.0	0.0	0.0	3.369607409756653	0.0
23	0.0	0.0	0.0	3.4986095685682903	0.0
24	0.0	0.0	0.0	3.597305097758779	0.0
25	0.0	0.0	0.0	3.6776329726334196	0.0
26	0.0	0.0	0.0	3.7654303602631716	0.0
27	0.0	0.0	0.0	3.857513533899955	0.0
28	0.0	0.0	0.0	3.9410863610370623	0.0
29	0.0	0.0	0.0	4.032924632616301	0.0
30	0.0	0.0	0.0	4.16970243175498	0.0
31	0.0	0.0	0.0	4.278561396333571	0.0
32	0.0	0.0	0.0	4.379767171613892	0.0
33	0.0	0.0	0.0	4.480666819322282	0.0
34	0.0	0.0	0.0	4.596199364968965	0.0
35	0.0	0.0	0.0	4.735364959168704	0.0
36	0.0	0.0	0.0	4.8504077007002975	0.0
37	0.0	0.0	0.0	4.9690015220662875	0.0
38	6.122551438615911E-5	0.0	0.0	5.100146573881441	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	65	0.0	44.000004	31
ATCGCTA	20	7.8574556E-4	44.000004	14
TCGAATA	20	7.8574556E-4	44.000004	39
TGCGTAA	20	7.8574556E-4	44.000004	30
ATTCGAC	25	4.443666E-5	44.0	18
CGTAACA	25	4.443666E-5	44.0	33
GTTACGG	180	0.0	41.555557	2
CGTTTTT	5330	0.0	39.831142	1
ATTACCG	50	1.3478711E-9	39.600002	1
TAACGGG	445	0.0	38.5618	3
TCGATTG	75	0.0	38.13333	1
GCATATC	480	0.0	37.125004	28
GGGCGAT	3400	0.0	36.947056	7
TAGGGCG	650	0.0	36.892307	5
ACGTCCA	30	1.3013349E-4	36.666668	17
GTACGGA	30	1.3013349E-4	36.666668	3
ATAGGGC	885	0.0	36.54237	4
AGGGACC	1575	0.0	36.31746	6
GCGATAA	195	0.0	36.102566	9
AGGGCGA	1775	0.0	35.94366	6
>>END_MODULE