FastQCFastQC Report
Thu 26 May 2016
SRR1546624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546624_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3559390
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433901.2190291033014082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT444400.043.2178231
AATCGCG750.041.0666661
GCGTAAG2950.040.2711871
AGGGCGA33850.039.4505166
CTAAGCG1200.038.51
TACGGGT2300.038.2608684
GTTTTTT510500.038.0442732
CGTAAGG5100.037.529412
TAGGGAC45600.037.43865
TGGGCGA19900.037.3668336
TAGGGAT91550.036.8869485
TTAGGGA66200.036.821754
CGAGGGA27550.036.57354
GGCGATA16500.036.48
CGACGGT1150.036.34782428
AGGGATA61600.036.321436
AAGGGAT94250.036.3204275
GGGCGAT73550.036.312717
AGGGATC63650.036.188536
GTAGGGA63600.035.9402544
Produced by FastQC (version 0.11.5)