##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546624_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3559390
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02380351689475	31.0	31.0	33.0	30.0	34.0
2	31.41761593980991	31.0	31.0	34.0	30.0	34.0
3	31.461816491027957	31.0	31.0	34.0	30.0	34.0
4	35.29242903980738	37.0	35.0	37.0	33.0	37.0
5	35.138754674255985	37.0	35.0	37.0	32.0	37.0
6	35.213717238066074	37.0	35.0	37.0	32.0	37.0
7	35.76646279278191	37.0	35.0	37.0	35.0	37.0
8	35.81230716499176	37.0	35.0	37.0	35.0	37.0
9	37.57078909588441	39.0	37.0	39.0	35.0	39.0
10	36.874485234829564	39.0	37.0	39.0	32.0	39.0
11	36.472804890725655	39.0	35.0	39.0	32.0	39.0
12	36.31688407283271	38.0	35.0	39.0	32.0	39.0
13	36.31415158215312	38.0	35.0	39.0	32.0	39.0
14	37.363305229266814	39.0	36.0	41.0	32.0	41.0
15	37.4449731555126	39.0	36.0	41.0	32.0	41.0
16	37.406943043611406	39.0	36.0	41.0	32.0	41.0
17	37.2889509157468	39.0	36.0	41.0	32.0	41.0
18	37.08172383470201	39.0	36.0	40.0	32.0	41.0
19	36.918564978830645	38.0	36.0	40.0	31.0	41.0
20	36.66578149626762	38.0	35.0	40.0	31.0	41.0
21	36.58876155745788	38.0	35.0	40.0	31.0	41.0
22	36.521799240881165	38.0	35.0	40.0	31.0	41.0
23	36.30916926776779	38.0	35.0	40.0	30.0	41.0
24	36.231248893771124	38.0	35.0	40.0	30.0	41.0
25	36.10280469406275	38.0	35.0	40.0	30.0	41.0
26	36.01329946985298	38.0	35.0	40.0	30.0	41.0
27	35.863834814392355	38.0	34.0	40.0	30.0	41.0
28	35.784289442853975	38.0	34.0	40.0	29.0	41.0
29	35.64942700856046	38.0	34.0	40.0	29.0	41.0
30	35.520087992605475	38.0	34.0	40.0	27.0	41.0
31	35.32929575011448	38.0	34.0	40.0	27.0	41.0
32	34.8881774686112	38.0	34.0	40.0	24.0	41.0
33	34.382408502580496	38.0	33.0	40.0	22.0	41.0
34	33.99693065384799	38.0	33.0	40.0	18.0	41.0
35	33.61918615268347	38.0	33.0	40.0	16.0	41.0
36	33.41742489583889	38.0	32.0	40.0	15.0	41.0
37	33.45244887466673	38.0	32.0	40.0	15.0	41.0
38	33.35812063302982	38.0	32.0	40.0	15.0	41.0
39	33.25956498164011	38.0	32.0	40.0	14.0	41.0
40	33.290331489384414	38.0	32.0	40.0	15.0	41.0
41	33.28344688275238	38.0	32.0	40.0	14.0	41.0
42	33.0429773079095	37.0	31.0	40.0	11.0	41.0
43	33.11138144457337	37.0	32.0	40.0	12.0	41.0
44	32.9128628781898	37.0	31.0	40.0	10.0	41.0
45	32.76921214028246	37.0	31.0	40.0	10.0	41.0
46	32.85406122959271	37.0	31.0	40.0	10.0	41.0
47	32.75148663113623	37.0	31.0	40.0	10.0	41.0
48	32.78457460407542	37.0	31.0	40.0	10.0	41.0
49	32.70311289293952	37.0	31.0	40.0	10.0	41.0
50	32.2812970761844	36.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	20.0
15	83.0
16	318.0
17	1024.0
18	2549.0
19	5245.0
20	9216.0
21	14560.0
22	21908.0
23	32658.0
24	46600.0
25	70303.0
26	99050.0
27	112359.0
28	107985.0
29	103565.0
30	107877.0
31	122289.0
32	144287.0
33	176306.0
34	233280.0
35	287779.0
36	293532.0
37	359332.0
38	502282.0
39	704972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.833227041712206	22.462388218205927	27.00614431124434	21.698240428837526
2	36.65507291979805	23.883952025487513	27.4551257378371	12.005849316877331
3	25.031732965480042	23.841753783653942	39.1117860082767	12.01472724258932
4	21.58440069787239	26.656814791298512	38.54323353158828	13.215550979240826
5	19.87933325654115	30.856972683521615	37.02353493154726	12.240159128389976
6	19.43830263050691	38.04618207052332	33.53998859355114	8.975526705418625
7	82.70009748861462	2.299972748139428	14.096179401526665	0.9037503617192834
8	83.6146924051593	1.6611273279972132	13.829532588449144	0.8946476783943317
9	77.86837070396894	5.260648594281604	15.079465863532796	1.7915148382166606
10	30.38009321821998	41.80764119694666	19.804460876723258	8.007804708110097
11	18.950466231573387	24.700299770466287	41.59485192687511	14.754382071085214
12	21.260862114013918	23.498296056346735	39.38565315967062	15.855188669968731
13	19.414281660621626	25.28742284492568	39.89096446301192	15.40733103144078
14	17.682692820960895	27.482546166618437	37.99162215997685	16.84313885244382
15	16.72011215404887	25.579045847743576	41.53276263629442	16.168079361913136
16	19.21846159032868	24.82085413511866	40.490027785659905	15.47065648889276
17	19.47474145850834	23.91024866620404	38.20247289563661	18.412536979651005
18	19.211775051343068	24.61817895763038	39.16797541151714	17.00207057950941
19	20.50087234048531	25.048617881153795	37.38873795790852	17.061771820452382
20	21.830257431750947	25.474926883539034	36.84721258417875	15.847603100531272
21	21.16843054568339	25.485546680751476	37.512101792722916	15.833920980842223
22	20.454853219231385	24.49130328511346	37.31571982839756	17.73812366725759
23	18.147969174493383	26.62638822944381	37.927819092597325	17.29782350346548
24	17.263800819803393	25.703224428904953	40.73442359505421	16.298551156237444
25	17.64260168174884	26.716796979257683	38.380677588013675	17.2599237509798
26	17.833308516346904	28.374552943060465	37.00181772719483	16.790320813397802
27	17.236071349304236	27.441780754567496	37.683029957380334	17.63911793874793
28	16.83692992338575	26.994175968354128	39.32553049820334	16.84336361005678
29	17.4981949154209	24.98998423887239	39.39520535822149	18.116615487485216
30	18.041293592441402	26.656646223088785	38.39761869309067	16.904441491379142
31	19.43167228092454	26.566293662678152	36.87128974346728	17.130744312930023
32	19.553912327674126	26.814791298509018	36.42264545329396	17.2086509205229
33	20.022335287788078	27.5637679490025	35.144589381888466	17.269307381320957
34	18.592904964052828	28.067225002036867	34.663074290819495	18.67679574309081
35	18.85328665866904	28.61748782797052	34.20440019216776	18.324825321192677
36	20.11409258327972	29.408297489176515	32.2868806171844	18.19072931035936
37	19.43830263050691	30.123476213620876	32.64298096022071	17.795240195651502
38	19.264930226808527	30.133000317470128	32.59361856947398	18.008450886247363
39	19.450270973397128	30.538772092970984	31.652052739373882	18.358904194258006
40	20.773109999185255	29.381663712040545	31.66961192788652	18.17561436088768
41	18.875003863021472	29.055175184511956	32.17320945442899	19.896611498037586
42	19.307549889166403	29.29339015954981	31.367341033154556	20.03171891812923
43	19.76119503622812	29.250601928982213	31.475252782077828	19.512950252711843
44	19.300357645551625	30.14373249348905	31.628481284714518	18.927428576244807
45	18.821005846507408	30.528292769266645	31.019331964184875	19.631369420041075
46	19.21096030499608	30.543913423367485	31.069228154262383	19.175898117374043
47	18.992243052882657	29.195901544927644	32.13137644371648	19.68047895847322
48	19.303026642205545	28.64162117666229	32.5402948257988	19.515057355333358
49	19.395739157552278	28.577649541073047	32.50065320181267	19.525958099562004
50	18.906104697715058	29.57981564256797	32.414571036048315	19.09950862366866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1927.0
1	2738.0
2	3549.0
3	44388.0
4	85227.0
5	66293.0
6	47359.0
7	47983.5
8	48608.0
9	49177.0
10	49746.0
11	49310.5
12	48875.0
13	47840.5
14	46806.0
15	44778.5
16	42751.0
17	40960.0
18	39169.0
19	37855.0
20	36541.0
21	36282.5
22	36024.0
23	38454.5
24	40885.0
25	46795.5
26	52706.0
27	62547.5
28	72389.0
29	83849.0
30	95309.0
31	107505.5
32	119702.0
33	133978.0
34	148254.0
35	163560.5
36	178867.0
37	188782.0
38	198697.0
39	208542.0
40	218387.0
41	232696.0
42	247005.0
43	255943.5
44	264882.0
45	262446.5
46	260011.0
47	251389.5
48	242768.0
49	230624.5
50	218481.0
51	196951.5
52	175422.0
53	160091.5
54	144761.0
55	129365.5
56	113970.0
57	102613.0
58	91256.0
59	79269.5
60	67283.0
61	58503.0
62	49723.0
63	40642.5
64	31562.0
65	24903.0
66	18244.0
67	13836.5
68	9429.0
69	7186.5
70	4944.0
71	4163.5
72	3383.0
73	2624.5
74	1866.0
75	1530.5
76	1195.0
77	973.0
78	751.0
79	592.0
80	433.0
81	273.0
82	113.0
83	79.0
84	45.0
85	53.5
86	62.0
87	35.5
88	9.0
89	7.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3559390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.910685616525175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.42549613232143	20.297518138873375
2	6.529767844789706	3.5144105924006186
3	2.9872148120389634	2.411639960274236
4	2.0105820496659668	2.1642456577915854
5	1.4080993459793378	1.8946459408242335
6	1.0672664412721586	1.7232523002085613
7	0.9688828843265773	1.8251311889541255
8	0.7860989582645959	1.6923569543469186
9	0.6680477234433844	1.6179860036178244
>10	7.17659327059489	41.97812554070073
>50	0.8718228535140485	15.646353009052316
>100	0.09842783730162506	3.652677543025552
>500	0.0012748848654632666	0.23172212433555278
>1k	3.187212163579365E-4	0.10941401231526103
>5k	0.0	0.0
>10k+	1.062404054526455E-4	1.2405210332791157
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43390	1.2190291033014082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.809470162022144E-5	0.0	0.0	0.019778669940635894	0.0
2	2.809470162022144E-5	0.0	0.0	0.06363449916980157	0.0
3	2.809470162022144E-5	0.0	0.0	0.10636654033415838	0.0
4	2.809470162022144E-5	0.0	0.0	0.15123377882165204	0.0
5	2.809470162022144E-5	0.0	0.0	0.24248536968413126	0.0
6	2.809470162022144E-5	0.0	0.0	0.4427444028330697	0.0
7	2.809470162022144E-5	0.0	0.0	0.5678501091479158	0.0
8	2.809470162022144E-5	0.0	0.0	0.8182862793905697	0.0
9	2.809470162022144E-5	0.0	0.0	0.9646596748319235	0.0
10	2.809470162022144E-5	0.0	0.0	1.1512646829934343	0.0
11	2.809470162022144E-5	0.0	0.0	1.2763141999050398	0.0
12	2.809470162022144E-5	0.0	0.0	1.3921767493868331	0.0
13	5.618940324044288E-5	0.0	0.0	1.4699990728748464	0.0
14	5.618940324044288E-5	0.0	0.0	1.5209347669123081	0.0
15	5.618940324044288E-5	0.0	0.0	1.5604640120919595	0.0
16	5.618940324044288E-5	0.0	0.0	1.621682366922422	0.0
17	5.618940324044288E-5	0.0	0.0	1.6902896282790028	0.0
18	5.618940324044288E-5	0.0	0.0	1.7941557401689616	0.0
19	5.618940324044288E-5	0.0	0.0	1.8481256619814068	0.0
20	5.618940324044288E-5	0.0	0.0	1.906506451948227	0.0
21	5.618940324044288E-5	0.0	0.0	1.9714052126909387	0.0
22	5.618940324044288E-5	0.0	0.0	2.049480388493534	0.0
23	5.618940324044288E-5	0.0	0.0	2.1350849443303486	0.0
24	5.618940324044288E-5	0.0	0.0	2.2076535586153807	0.0
25	5.618940324044288E-5	0.0	0.0	2.271512815398144	0.0
26	5.618940324044288E-5	0.0	0.0	2.339895319141763	0.0
27	5.618940324044288E-5	0.0	0.0	2.4112839559587456	0.0
28	8.428410486066432E-5	0.0	0.0	2.4916909919958194	0.0
29	8.428410486066432E-5	0.0	0.0	2.5755536763321807	0.0
30	8.428410486066432E-5	0.0	0.0	2.6901519642410636	0.0
31	8.428410486066432E-5	0.0	0.0	2.7858706126611583	0.0
32	8.428410486066432E-5	0.0	0.0	2.886674402074513	0.0
33	8.428410486066432E-5	0.0	0.0	2.9805107054860525	0.0
34	8.428410486066432E-5	0.0	0.0	3.0849106167067952	0.0
35	8.428410486066432E-5	0.0	0.0	3.1988346317767933	0.0
36	8.428410486066432E-5	0.0	0.0	3.3123091316208675	0.0
37	8.428410486066432E-5	0.0	0.0	3.4274693135621552	0.0
38	8.428410486066432E-5	0.0	0.0	3.547855110004804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	44440	0.0	43.217823	1
AATCGCG	75	0.0	41.066666	1
GCGTAAG	295	0.0	40.271187	1
AGGGCGA	3385	0.0	39.450516	6
CTAAGCG	120	0.0	38.5	1
TACGGGT	230	0.0	38.260868	4
GTTTTTT	51050	0.0	38.044273	2
CGTAAGG	510	0.0	37.52941	2
TAGGGAC	4560	0.0	37.4386	5
TGGGCGA	1990	0.0	37.366833	6
TAGGGAT	9155	0.0	36.886948	5
TTAGGGA	6620	0.0	36.82175	4
CGAGGGA	2755	0.0	36.5735	4
GGCGATA	1650	0.0	36.4	8
CGACGGT	115	0.0	36.347824	28
AGGGATA	6160	0.0	36.32143	6
AAGGGAT	9425	0.0	36.320427	5
GGGCGAT	7355	0.0	36.31271	7
AGGGATC	6365	0.0	36.18853	6
GTAGGGA	6360	0.0	35.940254	4
>>END_MODULE