FastQCFastQC Report
Thu 26 May 2016
SRR1546623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546623_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2863224
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109430.38219154351877466No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCC207.8584504E-444.00000426
CCATACG207.8584504E-444.0000041
TCGCGAG600.044.01
ATCGACG254.44451E-544.01
CGTTTTT113300.041.9805831
TACGGGA12850.039.8910524
TTGCGAG2550.039.6862751
TCGATAG1400.039.2857131
CGTAAGG5100.039.25492
GCGAACG452.3530447E-839.111111
TACAACG404.1274143E-738.5000041
TAGGGAC32650.038.0030635
TATTGCG2100.037.7142871
AGTAGCG2100.037.7142871
ACTACGG2300.037.3043442
TATCGCG651.0913936E-1137.230771
TCGAACG950.037.052631
TGGGCGA17500.036.9600036
TATGGGA52650.036.8547024
AATGCGG4600.036.8260842
Produced by FastQC (version 0.11.5)