FastQCFastQC Report
Thu 26 May 2016
SRR1546622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546622_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1736811
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56330.32433005088060823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT302.528468E-644.028
CGCGGAT254.443784E-544.04
CTATCGA351.4466605E-744.043
GCGAACG302.528468E-644.01
TAACGCG408.3164196E-944.01
CCGCTAT207.857594E-444.042
ACTACCG254.443784E-544.01
TACGCAG950.041.684211
CGTTTTT58650.041.4492761
CGTTAGG3400.041.4117662
CGAAAGG2550.041.4117622
GCGTAAG1600.041.251
TACGGGA7850.040.076434
GTTTACG1100.040.01
CGAACGG1050.039.809522
GCGAAAG1500.039.61
TATTGCG1000.039.61
TATAGCG950.039.368421
ACGGGTA404.1261592E-738.55
ACGGGAT12700.037.9370085
Produced by FastQC (version 0.11.5)