##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546622_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1736811
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.067878427762146	31.0	31.0	33.0	30.0	34.0
2	31.44283287012807	31.0	31.0	34.0	30.0	34.0
3	31.590088961896257	31.0	31.0	34.0	30.0	34.0
4	35.35635195769718	37.0	35.0	37.0	33.0	37.0
5	35.14933691691267	37.0	35.0	37.0	32.0	37.0
6	35.228700186721525	37.0	35.0	37.0	32.0	37.0
7	35.777349406469675	37.0	35.0	37.0	35.0	37.0
8	35.83003619852707	37.0	35.0	37.0	35.0	37.0
9	37.605038199320475	39.0	37.0	39.0	35.0	39.0
10	36.85471936785292	39.0	37.0	39.0	32.0	39.0
11	36.427127073700014	38.0	35.0	39.0	32.0	39.0
12	36.031744386694925	37.0	35.0	39.0	31.0	39.0
13	35.90647456746877	38.0	35.0	39.0	31.0	39.0
14	36.88685009480018	39.0	35.0	41.0	31.0	41.0
15	37.06357974471604	39.0	35.0	41.0	32.0	41.0
16	37.10378734358546	39.0	35.0	41.0	32.0	41.0
17	37.01409652518323	38.0	35.0	41.0	32.0	41.0
18	36.94712665914714	38.0	35.0	40.0	31.0	41.0
19	36.929866865191435	38.0	35.0	40.0	31.0	41.0
20	36.81029196613794	38.0	35.0	40.0	31.0	41.0
21	36.72233766368361	38.0	35.0	40.0	31.0	41.0
22	36.66021921786539	38.0	35.0	40.0	31.0	41.0
23	36.46430613348257	38.0	35.0	40.0	30.0	41.0
24	36.44506224338745	38.0	35.0	40.0	30.0	41.0
25	36.30841697801315	38.0	35.0	40.0	30.0	41.0
26	36.205938930603274	38.0	35.0	40.0	30.0	41.0
27	36.043957575118995	38.0	34.0	40.0	30.0	41.0
28	36.01834684372681	38.0	34.0	40.0	30.0	41.0
29	35.930790396882564	38.0	34.0	40.0	30.0	41.0
30	35.91556997278345	38.0	34.0	40.0	30.0	41.0
31	35.86527089015443	38.0	34.0	40.0	29.0	41.0
32	35.68529793972977	38.0	34.0	40.0	29.0	41.0
33	35.50251006010441	38.0	34.0	40.0	27.0	41.0
34	35.418199792608405	38.0	34.0	40.0	27.0	41.0
35	35.21269556676	38.0	34.0	40.0	27.0	41.0
36	35.04473428599888	38.0	34.0	40.0	25.0	41.0
37	35.12165514843008	38.0	34.0	40.0	26.0	41.0
38	35.09156839748251	38.0	34.0	40.0	26.0	41.0
39	35.018947369633196	38.0	34.0	40.0	25.0	41.0
40	35.02148880908746	38.0	34.0	40.0	25.0	41.0
41	35.057940098260545	38.0	34.0	40.0	25.0	41.0
42	34.83593839513914	38.0	34.0	40.0	24.0	41.0
43	34.90526487913768	38.0	34.0	40.0	25.0	41.0
44	34.72665246823057	38.0	34.0	40.0	24.0	41.0
45	34.501414373814995	37.0	33.0	40.0	23.0	41.0
46	34.5958207312137	37.0	33.0	40.0	24.0	41.0
47	34.5001557452135	37.0	33.0	40.0	23.0	41.0
48	34.54996484937048	37.0	33.0	40.0	24.0	41.0
49	34.47658956558889	37.0	33.0	40.0	24.0	41.0
50	34.015761070145224	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	11.0
15	24.0
16	108.0
17	297.0
18	727.0
19	1479.0
20	2666.0
21	4369.0
22	6657.0
23	9760.0
24	13980.0
25	19674.0
26	25987.0
27	31196.0
28	35886.0
29	41817.0
30	50074.0
31	61094.0
32	75358.0
33	94572.0
34	136873.0
35	180118.0
36	142835.0
37	177762.0
38	251402.0
39	372081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.101298874776816	24.250019144282252	28.9032024785656	13.74547950237533
2	30.35459816871266	26.457455647160227	30.107075553989464	13.080870630137648
3	27.598166985354194	26.533629738641686	32.28975403771625	13.578449238287874
4	24.030190964935162	29.31700685912284	32.112993296334494	14.539808879607511
5	22.025194451209718	33.687488160772816	30.822467153881455	13.46485023413601
6	20.957260173962506	42.46731509646127	26.789328257363636	9.786096472212579
7	90.84005110515767	2.950292230991167	5.251809206643671	0.9578474572074912
8	91.66132641951255	2.361454412713876	4.836853290311957	1.1403658774616237
9	85.84699198703831	5.578269598707056	6.522931971296819	2.051806442957812
10	39.0275050077412	40.3495256536261	11.783262542671597	8.839706795961105
11	27.325425737170022	25.865163221559513	32.32660318249942	14.482807858771046
12	28.4809918868547	24.839432730446777	30.860813295171436	15.818762087527084
13	21.093832316815128	32.447687169185365	31.19579505196593	15.262685462033579
14	17.991652517170838	34.35157884191199	30.648124637626083	17.00864400329109
15	16.11320978506009	27.58054848800474	40.32770405069982	15.978537676235351
16	19.164664433838798	25.965634717882374	38.60725202684691	16.262448821431924
17	19.226962519237844	24.668314514359942	32.05023459662566	24.05448836977656
18	20.019737323174486	25.89746380003351	35.3308448645247	18.751954012267312
19	23.99552973812349	26.237800198179308	30.841928108470064	18.924741955227137
20	25.932643217943692	26.664501779410653	30.129760808746603	17.27309419389905
21	22.294308361704296	28.24648162638307	31.742889698418537	17.716320313494098
22	22.362421702764436	26.326871490334874	29.923463174749582	21.387243632151108
23	18.761799643139064	30.488694509650156	30.37682280916001	20.372683038050774
24	18.44126966031422	26.864984157746584	37.09994927484914	17.593796907090066
25	18.137264215853076	28.753445251095254	34.69105158822693	18.41823894482474
26	18.264566495721184	32.50232754168415	30.55271989871091	18.68038606388375
27	18.387492939646283	32.794644898034385	31.172591606110277	17.645270556209052
28	17.081018026716784	30.306463973339643	35.66289020509428	16.949627794849295
29	17.639224993393064	27.3363077502388	35.43125878405883	19.593208472309307
30	18.65441893216936	30.484779288016945	32.57429852758878	18.28650325222491
31	22.680591037251606	29.065799329921333	30.06642634115053	18.187183291676526
32	23.770346917425098	28.689650169189395	30.830527904302773	16.709475009082738
33	23.901794726081306	29.270427237045364	29.10616065881665	17.72161737805668
34	20.222234889115743	29.114336562815414	30.178470772006854	20.48495777606199
35	19.933314563300208	28.468440146912933	31.9881668183815	19.61007847140535
36	24.49235984802031	28.523541133721515	28.857083470797917	18.12701554746026
37	20.23398055401538	32.14316353362571	30.128666849760855	17.49418906259806
38	20.000449098952046	32.00117917263306	28.775727468331326	19.222644260083566
39	20.438953921871754	31.59278701021585	29.997449348259543	17.97080971965286
40	22.723140284118422	28.436888066692344	29.408841837137146	19.431129812052088
41	18.368838060099804	28.355819948169376	30.85568896097503	22.419653030755793
42	19.913335417613084	28.870268555415645	29.3875960020981	21.828800024873175
43	20.48870026732903	28.948630564868598	29.355583307567723	21.20708586023465
44	19.43550564799509	30.486621745256105	30.750496167976827	19.327376438771978
45	18.932342091338665	33.322566473842	28.01565628038975	19.729435154429584
46	20.327139798170325	31.75146863993837	29.206229117618438	18.715162444272863
47	20.02371012159642	29.686649842729	29.875559286531466	20.414080749143114
48	20.62342995294249	28.220284187513784	32.169533702861166	18.986752156682563
49	20.655845684993935	27.749421209331356	31.916253409265604	19.67847969640911
50	19.137085152040147	31.28912702648705	30.133963914323438	19.439823907149368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1386.0
1	1748.5
2	2111.0
3	6935.5
4	11760.0
5	9436.5
6	7113.0
7	7518.0
8	7923.0
9	8298.0
10	8673.0
11	8980.0
12	9287.0
13	9326.5
14	9366.0
15	9374.0
16	9382.0
17	9453.5
18	9525.0
19	9466.0
20	9407.0
21	10744.0
22	12081.0
23	14042.5
24	16004.0
25	19586.0
26	23168.0
27	27575.5
28	31983.0
29	38049.5
30	44116.0
31	52706.5
32	61297.0
33	67496.5
34	73696.0
35	83548.0
36	93400.0
37	97562.0
38	101724.0
39	109326.5
40	116929.0
41	132134.5
42	147340.0
43	154241.5
44	161143.0
45	158718.0
46	156293.0
47	148443.5
48	140594.0
49	131015.5
50	121437.0
51	107187.0
52	92937.0
53	80820.0
54	68703.0
55	60357.0
56	52011.0
57	46064.0
58	40117.0
59	36816.0
60	33515.0
61	29370.0
62	25225.0
63	20237.0
64	15249.0
65	12394.5
66	9540.0
67	7182.0
68	4824.0
69	3789.0
70	2754.0
71	2304.5
72	1855.0
73	1470.5
74	1086.0
75	1049.5
76	1013.0
77	704.5
78	396.0
79	347.5
80	299.0
81	199.0
82	99.0
83	69.5
84	40.0
85	23.0
86	6.0
87	4.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1736811.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.617619613961843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39417762170892	16.94695511775463
2	5.804284417683971	2.5094962534547616
3	2.2352808708852305	1.449643547914868
4	1.3763401451963346	1.190127908731175
5	0.9654954664211519	1.0435856866048567
6	0.7924067442719473	1.0277968546325336
7	0.6451659235058808	0.9762866065568372
8	0.5593974883118967	0.9674273692265799
9	0.456604079800909	0.8883623980187213
>10	6.636411964964914	34.15549784030993
>50	1.659290333573181	24.919912568189893
>100	0.47380272632463244	13.369318209513
>500	5.368869404291034E-4	0.06963730592340088
>1k	5.368869404291034E-4	0.1590632129467015
>5k	2.684434702145517E-4	0.32688912022209765
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5633	0.32433005088060823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02821262647461353	0.0
2	0.0	0.0	0.0	0.08970463683152628	0.0
3	0.0	0.0	0.0	0.13461453203601312	0.0
4	0.0	0.0	0.0	0.22506766712094753	0.0
5	0.0	0.0	0.0	0.3666489905925285	0.0
6	0.0	0.0	0.0	0.6202747449204318	0.0
7	0.0	0.0	0.0	0.7451012228734157	0.0
8	0.0	0.0	0.0	1.0710434238382875	0.0
9	0.0	0.0	0.0	1.2281129034765441	0.0
10	0.0	0.0	0.0	1.4752900574673928	0.0
11	0.0	0.0	0.0	1.6848695684216648	0.0
12	0.0	0.0	0.0	1.8614575794372559	0.0
13	0.0	0.0	0.0	1.9606048096194693	0.0
14	0.0	0.0	0.0	2.007069278119496	0.0
15	0.0	0.0	0.0	2.0558943949571944	0.0
16	0.0	0.0	0.0	2.1460596460985104	0.0
17	0.0	0.0	0.0	2.250388787265857	0.0
18	0.0	0.0	0.0	2.390818574962964	0.0
19	0.0	0.0	0.0	2.462213792980353	0.0
20	0.0	0.0	0.0	2.538560614827981	0.0
21	0.0	0.0	0.0	2.629301633856534	0.0
22	0.0	0.0	0.0	2.7383520716992233	0.0
23	0.0	0.0	0.0	2.8573057172023897	0.0
24	0.0	0.0	0.0	2.949198272005417	0.0
25	0.0	0.0	0.0	3.0318785406126514	0.0
26	0.0	0.0	0.0	3.1133496966566887	0.0
27	0.0	0.0	0.0	3.195914811686476	0.0
28	0.0	0.0	0.0	3.28371941449012	0.0
29	0.0	0.0	0.0	3.3840181804468075	0.0
30	0.0	0.0	0.0	3.5086719280336203	0.0
31	0.0	0.0	0.0	3.615995062214599	0.0
32	0.0	0.0	0.0	3.7156604834953257	0.0
33	0.0	0.0	0.0	3.815556211930947	0.0
34	0.0	0.0	0.0	3.924779380139808	0.0
35	0.0	0.0	0.0	4.045920943614475	0.0
36	0.0	0.0	0.0	4.163492746188273	0.0
37	0.0	0.0	0.0	4.289182875972112	5.757678872370108E-5
38	0.0	0.0	0.0	4.41095778412274	5.757678872370108E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	30	2.528468E-6	44.0	28
CGCGGAT	25	4.443784E-5	44.0	4
CTATCGA	35	1.4466605E-7	44.0	43
GCGAACG	30	2.528468E-6	44.0	1
TAACGCG	40	8.3164196E-9	44.0	1
CCGCTAT	20	7.857594E-4	44.0	42
ACTACCG	25	4.443784E-5	44.0	1
TACGCAG	95	0.0	41.68421	1
CGTTTTT	5865	0.0	41.449276	1
CGTTAGG	340	0.0	41.411766	2
CGAAAGG	255	0.0	41.411762	2
GCGTAAG	160	0.0	41.25	1
TACGGGA	785	0.0	40.07643	4
GTTTACG	110	0.0	40.0	1
CGAACGG	105	0.0	39.80952	2
GCGAAAG	150	0.0	39.6	1
TATTGCG	100	0.0	39.6	1
TATAGCG	95	0.0	39.36842	1
ACGGGTA	40	4.1261592E-7	38.5	5
ACGGGAT	1270	0.0	37.937008	5
>>END_MODULE