FastQCFastQC Report
Thu 26 May 2016
SRR1546621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546621_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3010459
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89810.2983266006944456No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30280.10058266862295749No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGA302.5290883E-644.034
GCGTAAG2050.041.853661
TATAGCG1900.041.684211
GGTCGTA750.041.0666668
CGTTTTT91050.039.9406931
TACGGGA13000.039.769234
CGTAAGG5500.038.82
ACCGCGT404.1275052E-738.524
TCGATCC404.1275052E-738.520
GCGATCG800.038.59
TATACGG3700.038.0540542
CGTTAGG4550.037.7142872
CGCACGA357.2941366E-637.71428739
CGTTTCG1050.037.71428742
TCGTAAG1650.037.3333321
GGCGATT15150.037.3201338
ACGTTAG651.0913936E-1137.230771
TTAGGGA46850.037.1910364
ACGGGAT17550.037.105415
GGGCGAT55550.037.02977
Produced by FastQC (version 0.11.5)