FastQCFastQC Report
Thu 26 May 2016
SRR1546620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546620_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3915907
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180990.4621917731958395No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA254.44481E-544.06
CGTTTTT191050.042.180581
GCGAACG900.041.5555571
CCGAACG501.3496901E-939.61
AGGGCGA41200.039.2475746
CGAACGG1850.039.243242
TATAGCG2700.039.111111
GTAAGCG2250.039.111111
CGTAAGG7000.038.3428572
TCGCGAG1100.038.01
CGCGTAC357.2947023E-637.71428337
TAGGGCG15350.037.693815
GGGCGAT83250.037.3669667
CGTTAGG5950.037.3445362
TAGGGAT104550.037.0349125
AGGGATC63900.037.0109566
CGGGATT27850.036.969486
CGAGGGA34350.036.57064
TGGGCGA24700.036.518226
TAAGGGA78000.036.4974374
Produced by FastQC (version 0.11.5)