##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546620_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3915907
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.006260618548907	31.0	31.0	33.0	30.0	34.0
2	31.384539265105122	31.0	31.0	34.0	30.0	34.0
3	31.50875263380872	31.0	31.0	34.0	30.0	34.0
4	35.28887279498721	37.0	35.0	37.0	33.0	37.0
5	35.09457757806812	37.0	35.0	37.0	32.0	37.0
6	35.16992946972438	37.0	35.0	37.0	32.0	37.0
7	35.74130105745616	37.0	35.0	37.0	35.0	37.0
8	35.79678960710763	37.0	35.0	37.0	35.0	37.0
9	37.558541865269014	39.0	37.0	39.0	35.0	39.0
10	36.79460697100314	39.0	37.0	39.0	32.0	39.0
11	36.39722138447108	38.0	35.0	39.0	32.0	39.0
12	36.083872012282214	38.0	35.0	39.0	31.0	39.0
13	35.996932000683366	38.0	35.0	39.0	31.0	39.0
14	36.99257311269139	39.0	35.0	41.0	31.0	41.0
15	37.12302615971217	39.0	35.0	41.0	31.0	41.0
16	37.13940984808883	39.0	35.0	41.0	32.0	41.0
17	37.04150813591845	39.0	35.0	41.0	31.0	41.0
18	36.92485368013081	38.0	35.0	40.0	31.0	41.0
19	36.88360755247763	38.0	35.0	40.0	31.0	41.0
20	36.75462185388979	38.0	35.0	40.0	31.0	41.0
21	36.67713380322873	38.0	35.0	40.0	31.0	41.0
22	36.609486384635794	38.0	35.0	40.0	31.0	41.0
23	36.4086488775142	38.0	35.0	40.0	30.0	41.0
24	36.3885227611381	38.0	35.0	40.0	30.0	41.0
25	36.24907792754016	38.0	35.0	40.0	30.0	41.0
26	36.15608235844212	38.0	35.0	40.0	30.0	41.0
27	35.98099750581411	38.0	34.0	40.0	30.0	41.0
28	35.938117273980204	38.0	34.0	40.0	30.0	41.0
29	35.824208031498195	38.0	34.0	40.0	29.0	41.0
30	35.781346696946585	38.0	34.0	40.0	29.0	41.0
31	35.719451202492806	38.0	34.0	40.0	29.0	41.0
32	35.48891406256584	38.0	34.0	40.0	27.0	41.0
33	35.24888563492443	38.0	34.0	40.0	26.0	41.0
34	35.13346818502074	38.0	34.0	40.0	25.0	41.0
35	34.91249894341209	38.0	34.0	40.0	25.0	41.0
36	34.72264841836132	38.0	33.0	40.0	24.0	41.0
37	34.81061603352684	38.0	34.0	40.0	24.0	41.0
38	34.77162966331938	38.0	33.0	40.0	24.0	41.0
39	34.684150568437914	38.0	33.0	40.0	24.0	41.0
40	34.70287496613173	38.0	33.0	40.0	23.0	41.0
41	34.72078448236896	38.0	34.0	40.0	24.0	41.0
42	34.485330984622465	38.0	33.0	40.0	23.0	41.0
43	34.56353457832375	38.0	33.0	40.0	23.0	41.0
44	34.385730815364106	38.0	33.0	40.0	23.0	41.0
45	34.21066128485687	37.0	33.0	40.0	23.0	41.0
46	34.282656099851195	37.0	33.0	40.0	23.0	41.0
47	34.14839729339844	37.0	33.0	40.0	23.0	41.0
48	34.21147846463157	37.0	33.0	40.0	23.0	41.0
49	34.12174523041533	37.0	33.0	40.0	23.0	41.0
50	33.64294197998063	37.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	8.0
14	26.0
15	81.0
16	293.0
17	863.0
18	2026.0
19	4417.0
20	7338.0
21	11550.0
22	17503.0
23	25537.0
24	36591.0
25	51727.0
26	68380.0
27	80511.0
28	88352.0
29	99082.0
30	115208.0
31	139219.0
32	169610.0
33	210539.0
34	292073.0
35	372574.0
36	327547.0
37	404012.0
38	567342.0
39	823491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.949788388743656	24.864558836560725	28.96062138350068	14.225031391194939
2	31.484276822713102	25.759345152987546	29.792255025464087	12.964122998835265
3	27.110883889734865	25.888102041238465	33.997870735949554	13.003143333077114
4	23.45745187513391	29.05495457374243	33.462260467370655	14.025333083753011
5	21.63299588064783	33.52962672504735	31.66288167722063	13.17449571708419
6	20.9035863211256	41.60011971683699	27.987692251118325	9.508601710919079
7	89.31637038366846	2.903746181919029	6.845463898912819	0.9344195354996939
8	90.25112189845163	2.1486720700976814	6.377270961746538	1.2229350697041579
9	84.08233392672501	5.794391950574925	7.9599694272616786	2.163304695438375
10	34.60546943530579	42.961668905824375	13.203709894029657	9.229151764840177
11	25.3078277905987	25.564269018646257	34.483454280196135	14.644448910558907
12	26.33801058094587	24.816932577816583	32.512263442415765	16.332793398821778
13	20.799676805399105	29.615948489072903	33.383351545376335	16.20102316015166
14	18.34208524359746	32.24346237027591	32.149027032562316	17.26542535356432
15	16.706857440689983	27.312982662764973	39.545219025885956	16.434940870659084
16	19.46236210410513	25.455916087894835	38.47379419378448	16.607927614215555
17	19.759560173415764	25.14158278018349	33.01758698559491	22.081270060805835
18	19.951393125526216	25.749768827502802	35.61621866913591	18.68261937783507
19	23.109333291112378	26.563066998271413	32.184880795177214	18.142718915439
20	24.561461750751487	26.56679538099347	31.538031929767484	17.33371093848756
21	22.101188817814112	27.731021191259135	32.82222994570606	17.345560045220687
22	21.757692406893216	25.910089284551447	31.52092222823474	20.8112960803206
23	18.59551312122581	29.335604752615424	32.13071709823548	19.938165027923286
24	17.733235237711213	26.840984732272755	37.45962301964781	17.966157010368224
25	17.9981291690533	28.15184323836087	35.3620757592047	18.487951833381132
26	18.434017968251034	31.672943203196603	31.65496524815324	18.238073580399124
27	18.066414753976538	31.233887832371916	32.43483565876309	18.264861754888457
28	17.33371093848756	29.34977771433285	35.7083301518652	17.608181195314394
29	17.518342493833487	26.648002621104126	35.75340272381341	20.080252161248975
30	18.733948482433316	29.79404260622124	33.32992841760542	18.14208049374002
31	21.525409055935192	28.741387372069866	31.178549439504057	18.55465413249089
32	22.67967548769672	28.531908444199516	31.360142107562822	17.428273960540945
33	22.58386115911333	28.802829076380004	30.55552647190038	18.057783292606285
34	19.528017391628556	29.142341735899247	30.968329942462887	20.361310930009317
35	19.476815971370108	28.60716559407565	31.969426240204378	19.946592194349865
36	22.868980289879204	28.95906363455516	29.588496356016623	18.58345971954901
37	20.144043257411372	31.100023570529128	30.787554454178817	17.968378717880682
38	19.77069424784603	30.932859232867376	30.211621471092137	19.084825048194453
39	20.095472134552736	31.290809511053254	30.06496323840173	18.548755115992286
40	21.942962383938127	29.05040901124567	29.887992743443604	19.118635861372603
41	18.849196367533754	28.377333782441717	31.0621779322134	21.711291917811124
42	19.961352504030355	28.64164036582074	29.78329158481036	21.61371554533854
43	20.04983775150942	28.679894594023814	30.085265048429395	21.185002606037376
44	19.31376306944981	30.264074197880593	30.5796843489899	19.842478383679694
45	19.050069370901813	32.0050757078756	28.872825631456518	20.072029289766075
46	20.05737112755742	31.647763851388707	29.06516932092616	19.22969570012771
47	19.77600591638157	29.524168985627085	30.154776403014676	20.545048694976668
48	20.581540879290543	28.320667472439975	31.24543049668953	19.852361151579952
49	20.019167973090273	28.498327462833007	31.766969951022837	19.715534613053883
50	19.41989429268877	30.718655984424554	30.256387600624834	19.60506212226184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2486.0
1	3213.0
2	3940.0
3	20510.5
4	37081.0
5	29430.0
6	21779.0
7	22360.0
8	22941.0
9	24017.5
10	25094.0
11	25681.0
12	26268.0
13	26010.5
14	25753.0
15	25735.5
16	25718.0
17	25379.5
18	25041.0
19	25062.5
20	25084.0
21	27781.0
22	30478.0
23	35191.0
24	39904.0
25	47422.0
26	54940.0
27	68457.0
28	81974.0
29	94477.0
30	106980.0
31	123100.5
32	139221.0
33	154674.0
34	170127.0
35	190753.0
36	211379.0
37	226136.0
38	240893.0
39	248414.5
40	255936.0
41	279629.5
42	303323.0
43	312878.5
44	322434.0
45	329744.5
46	337055.0
47	319706.0
48	302357.0
49	283445.0
50	264533.0
51	236739.0
52	208945.0
53	183005.0
54	157065.0
55	137486.5
56	117908.0
57	107111.0
58	96314.0
59	87836.0
60	79358.0
61	69686.5
62	60015.0
63	48536.0
64	37057.0
65	29102.0
66	21147.0
67	17057.5
68	12968.0
69	10962.5
70	8957.0
71	7219.0
72	5481.0
73	4374.5
74	3268.0
75	2850.0
76	2432.0
77	1768.5
78	1105.0
79	910.5
80	716.0
81	469.5
82	223.0
83	154.0
84	85.0
85	81.5
86	78.0
87	69.0
88	60.0
89	32.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3915907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.332463517113325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81693597217041	16.02542475184812
2	6.890982525019923	2.802213015740661
3	2.4285699983894906	1.4813643267303083
4	1.3816557941558352	1.1236986411152705
5	0.9433500607972023	0.9590315347512874
6	0.710807301475714	0.8671478114971636
7	0.5645903089450297	0.803565830111839
8	0.4749976786038725	0.7726298376741406
9	0.3955410892601657	0.7238092290211426
>10	5.028943643506307	23.930533388775547
>50	1.4494418733572256	21.04083664614877
>100	0.9111192862502563	28.545143441670916
>500	0.0026814095599458996	0.3510287383836268
>1k	2.5537233904246345E-4	0.10369120487420984
>5k	0.0	0.0
>10k+	1.2768616952123173E-4	0.46988160165706655
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18099	0.4621917731958395	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025383646751569944	0.0
2	0.0	0.0	0.0	0.0954568124319602	0.0
3	0.0	0.0	0.0	0.1481138341640902	0.0
4	0.0	0.0	0.0	0.23343251001619802	0.0
5	0.0	0.0	0.0	0.3682416359734795	0.0
6	0.0	0.0	0.0	0.6374768348686524	0.0
7	0.0	0.0	0.0	0.7708814330881709	0.0
8	0.0	0.0	0.0	1.1376419307200094	0.0
9	0.0	0.0	0.0	1.3216095274989932	0.0
10	0.0	0.0	0.0	1.6014680634652456	0.0
11	0.0	0.0	0.0	1.8250688793171033	0.0
12	0.0	0.0	0.0	2.016059114784902	0.0
13	0.0	0.0	0.0	2.1281148913904238	0.0
14	0.0	0.0	0.0	2.191242028985877	0.0
15	0.0	0.0	0.0	2.2502577308398797	0.0
16	0.0	0.0	0.0	2.3453059533844907	0.0
17	0.0	0.0	0.0	2.461779608146976	0.0
18	0.0	0.0	0.0	2.617401281491108	0.0
19	0.0	0.0	0.0	2.70180063009668	0.0
20	0.0	0.0	0.0	2.7931970805231074	0.0
21	0.0	0.0	0.0	2.8998645779892116	0.0
22	0.0	0.0	0.0	3.0217009750231556	0.0
23	0.0	0.0	0.0	3.160672610457807	0.0
24	0.0	0.0	0.0	3.2706343638906645	0.0
25	0.0	0.0	0.0	3.3713262342542865	0.0
26	0.0	0.0	0.0	3.467268247177474	0.0
27	0.0	0.0	0.0	3.568368707428445	0.0
28	2.5536867959325897E-5	0.0	0.0	3.674321172591688	0.0
29	2.5536867959325897E-5	0.0	0.0	3.7893647627484515	0.0
30	2.5536867959325897E-5	0.0	0.0	3.9445267724693154	0.0
31	2.5536867959325897E-5	0.0	0.0	4.070832121396141	0.0
32	2.5536867959325897E-5	0.0	0.0	4.192642981562126	0.0
33	2.5536867959325897E-5	0.0	0.0	4.31790131890262	0.0
34	2.5536867959325897E-5	0.0	0.0	4.451740043877447	0.0
35	2.5536867959325897E-5	0.0	0.0	4.598857940191123	0.0
36	2.5536867959325897E-5	0.0	0.0	4.74406057140785	0.0
37	2.5536867959325897E-5	0.0	0.0	4.893604470177663	0.0
38	2.5536867959325897E-5	0.0	0.0	5.044910412836669	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGA	25	4.44481E-5	44.0	6
CGTTTTT	19105	0.0	42.18058	1
GCGAACG	90	0.0	41.555557	1
CCGAACG	50	1.3496901E-9	39.6	1
AGGGCGA	4120	0.0	39.247574	6
CGAACGG	185	0.0	39.24324	2
TATAGCG	270	0.0	39.11111	1
GTAAGCG	225	0.0	39.11111	1
CGTAAGG	700	0.0	38.342857	2
TCGCGAG	110	0.0	38.0	1
CGCGTAC	35	7.2947023E-6	37.714283	37
TAGGGCG	1535	0.0	37.69381	5
GGGCGAT	8325	0.0	37.366966	7
CGTTAGG	595	0.0	37.344536	2
TAGGGAT	10455	0.0	37.034912	5
AGGGATC	6390	0.0	37.010956	6
CGGGATT	2785	0.0	36.96948	6
CGAGGGA	3435	0.0	36.5706	4
TGGGCGA	2470	0.0	36.51822	6
TAAGGGA	7800	0.0	36.497437	4
>>END_MODULE