FastQCFastQC Report
Thu 26 May 2016
SRR1546619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546619_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2402783
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98400.40952512149453363No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42250.17583776812138258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24090.10025874163418003No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTA207.858198E-444.00000433
CCCGTTA207.858198E-444.00000414
TATCGCT207.858198E-444.00000433
GACACGC254.4442942E-544.010
CGCGATC302.5288737E-644.039
GTTCGCT254.4442942E-544.025
ATACGAC254.4442942E-544.018
TACACCG302.5288737E-644.024
AAGTACG1100.042.01
AGTACGG3000.041.82
CCGGATA850.041.41176620
GCGATAC2400.041.259
CGGGTAT3850.041.142866
CGATACG1450.040.9655210
GTCGTTT3700.040.432439
CACGACG1200.040.33333226
ATGACGG3000.040.3333322
CGGTCTA1150.040.17391631
CGGGTCA2800.040.071436
GTTAGTC557.8216544E-1140.015
Produced by FastQC (version 0.11.5)