##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546619_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2402783
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.003144270622858	31.0	31.0	33.0	30.0	34.0
2	31.36597811787415	31.0	31.0	34.0	30.0	34.0
3	31.53162811623022	31.0	31.0	34.0	30.0	34.0
4	35.30440285285854	37.0	35.0	37.0	33.0	37.0
5	35.10491875462745	37.0	35.0	37.0	32.0	37.0
6	35.16665674761308	37.0	35.0	37.0	32.0	37.0
7	35.6629620735622	37.0	35.0	37.0	35.0	37.0
8	35.69631922649694	37.0	35.0	37.0	35.0	37.0
9	37.4596112091687	39.0	37.0	39.0	35.0	39.0
10	36.74911217534001	39.0	37.0	39.0	32.0	39.0
11	36.35091058992843	38.0	35.0	39.0	32.0	39.0
12	35.52780588176294	37.0	35.0	39.0	30.0	39.0
13	35.186873721014344	37.0	35.0	39.0	30.0	39.0
14	36.08223464207962	38.0	35.0	40.0	30.0	41.0
15	36.39762600284753	38.0	35.0	40.0	31.0	41.0
16	36.535327160213804	38.0	35.0	40.0	31.0	41.0
17	36.467750520958404	38.0	35.0	40.0	31.0	41.0
18	36.42665900333072	38.0	35.0	40.0	31.0	41.0
19	36.34973029191567	37.0	35.0	40.0	31.0	41.0
20	36.15907761957696	37.0	34.0	40.0	31.0	41.0
21	36.03529407358051	37.0	34.0	40.0	30.0	41.0
22	35.94164683202769	37.0	34.0	40.0	30.0	41.0
23	35.79271369907312	37.0	34.0	40.0	30.0	41.0
24	35.73908630117659	37.0	34.0	40.0	30.0	41.0
25	35.58571248423183	36.0	34.0	40.0	30.0	41.0
26	35.467841665268985	36.0	34.0	40.0	29.0	41.0
27	35.297048880402436	36.0	34.0	40.0	29.0	41.0
28	35.25088532755559	36.0	34.0	40.0	29.0	41.0
29	35.17948562146478	36.0	34.0	40.0	29.0	41.0
30	35.151298307004836	36.0	34.0	40.0	29.0	41.0
31	35.020780902811445	36.0	34.0	40.0	27.0	41.0
32	34.78189332952664	36.0	34.0	40.0	26.0	41.0
33	34.53280799805892	36.0	33.0	40.0	25.0	41.0
34	34.4183220041094	36.0	33.0	40.0	25.0	41.0
35	34.176779592664005	36.0	33.0	40.0	24.0	41.0
36	33.926665454183755	35.0	33.0	40.0	23.0	41.0
37	33.93900572794131	35.0	33.0	40.0	23.0	41.0
38	33.91432767753059	35.0	33.0	40.0	23.0	41.0
39	33.83746638793433	35.0	33.0	40.0	23.0	41.0
40	33.75157265554151	35.0	33.0	40.0	23.0	41.0
41	33.76977613042876	35.0	33.0	40.0	23.0	41.0
42	33.53385927901105	35.0	33.0	40.0	21.0	41.0
43	33.579401469046516	35.0	33.0	40.0	22.0	41.0
44	33.40180740416425	35.0	32.0	40.0	21.0	41.0
45	33.24365454558318	35.0	32.0	40.0	20.0	41.0
46	33.301640639208784	35.0	32.0	39.0	21.0	41.0
47	33.166418690327006	35.0	32.0	39.0	20.0	41.0
48	33.2356879501811	35.0	32.0	39.0	21.0	41.0
49	33.16055881866985	35.0	32.0	39.0	20.0	41.0
50	32.64332151509312	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	1.0
13	6.0
14	23.0
15	76.0
16	243.0
17	687.0
18	1689.0
19	3356.0
20	5759.0
21	9121.0
22	13840.0
23	19461.0
24	27235.0
25	36739.0
26	45966.0
27	53359.0
28	60111.0
29	69208.0
30	80975.0
31	96325.0
32	118442.0
33	148593.0
34	235604.0
35	322173.0
36	179966.0
37	211957.0
38	280279.0
39	381585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52649115629668	24.901582872860345	29.62069400357835	12.951231967264626
2	29.02334501284552	27.693470446561342	29.423838940095713	13.859345600497422
3	27.803467895353013	26.70844599782835	32.33958289200481	13.148503214813822
4	23.68957163422581	30.24442906413105	31.50854654789883	14.557452753744304
5	23.365114535936037	33.49153876983481	29.90523904988507	13.23810764434408
6	21.083926430310186	42.79221219727291	26.480127418913817	9.643733953503084
7	87.19047870739888	4.876012523810931	6.509576603463567	1.4239321653266235
8	87.39873721430524	4.434566084411284	5.876976822293149	2.289719878990321
9	81.58206546325657	7.04782745674495	8.559241512862377	2.8108655671361085
10	46.29594099841725	29.2306462963988	14.105684949493982	10.367727755689964
11	40.65864458005571	23.16110110650858	22.664676751916424	13.515577561519287
12	38.23961631158536	22.503613518157902	24.54395590446578	14.71281426579096
13	21.776831282725073	38.42848064099005	25.762209904098704	14.032478172186169
14	15.622842345729932	40.880262595498635	26.782776472115877	16.71411858665556
15	14.26225339533366	24.963219733117807	45.12808688924468	15.646439982303853
16	15.507517740886298	20.49177974040935	45.222435817133714	18.778266701570637
17	16.208954366665655	21.990833129749962	29.96995567223507	31.830256831349313
18	19.431051409969193	24.272520656255683	35.58835733397481	20.708070599800315
19	26.500062635702015	25.470839439100413	27.060288007697743	20.968809917499833
20	28.986221394108412	24.1991890237279	27.936355467805456	18.878234114358225
21	21.65617952182948	30.44398932404632	28.79315360563147	19.106677548492726
22	22.378466969343467	27.125961853400828	25.422603705786166	25.072967471469543
23	18.749008961691505	31.413032304623428	25.594945527748447	24.243013205936617
24	18.536172430052986	24.807608510631212	39.74890782896333	16.90731123035247
25	16.791903388695527	26.301959020019698	36.42942371408488	20.4767138771999
26	16.6588077242098	34.77205390582503	28.67337583127565	19.895762538689514
27	17.205715206075624	36.97179478962519	27.447921847291244	18.374568157007936
28	16.183483901792215	31.318766613547705	35.26881120767044	17.22893827698964
29	15.800885889404078	26.405505615779703	34.99874936687999	22.79485912793623
30	18.329994843479415	34.16862862772044	29.57291607273732	17.928460456062822
31	25.874496365256455	30.128438564780925	25.964184031600023	18.0328810383626
32	27.81765977202269	27.390821393359282	27.938894190611467	16.852624644006553
33	27.259806649206357	29.58194726698166	25.304573904509898	17.853672179302084
34	20.593120560616583	28.341843603854365	26.934725274816746	24.130310560712307
35	20.27607153871157	27.422118435164556	30.432294551776	21.86951547434787
36	29.49176017975822	26.27794519937922	25.168606569964915	19.061688050897647
37	20.42406659278012	34.563670543698706	28.06824419849816	16.944018665023016
38	20.4546977400789	33.154013491855075	25.490400090228704	20.900888677837322
39	20.579927525706648	33.95275395239604	27.153971041080283	18.313347480817036
40	25.0565698192471	26.32509885412041	26.894563512393756	21.72376781423874
41	17.808765918520315	25.90312982903575	29.517896539138157	26.770207713305776
42	20.28327152306305	26.03963820286726	26.87362945384581	26.803460820223883
43	20.062444257346584	26.454365625193788	28.781916635834364	24.701273481625265
44	18.998552928000574	29.393291029610246	29.930792751571822	21.677363290817357
45	17.49808451283366	36.096101895177384	25.13743438337961	21.26837920860935
46	20.72721506686205	33.04921834389539	26.38927443718388	19.834292152058673
47	20.305995173097198	28.943021487999545	27.944429438696712	22.806553900206552
48	21.015713861801085	26.67960444201578	31.74248361171192	20.562198084471213
49	21.328392951007228	25.873497523496713	32.73033811209751	20.067771413398546
50	20.189713344900476	32.29734020924903	27.713696992196134	19.799249453654365
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3476.0
1	4275.0
2	5074.0
3	11915.5
4	18757.0
5	14912.5
6	11068.0
7	12159.5
8	13251.0
9	14750.0
10	16249.0
11	17002.5
12	17756.0
13	17990.5
14	18225.0
15	17939.0
16	17653.0
17	17521.0
18	17389.0
19	17146.0
20	16903.0
21	17479.0
22	18055.0
23	18987.0
24	19919.0
25	20541.5
26	21164.0
27	24199.0
28	27234.0
29	32648.5
30	38063.0
31	48582.0
32	59101.0
33	62060.0
34	65019.0
35	71819.5
36	78620.0
37	84894.0
38	91168.0
39	106125.0
40	121082.0
41	160654.0
42	200226.0
43	227922.5
44	255619.0
45	246615.0
46	237611.0
47	220384.5
48	203158.0
49	190176.5
50	177195.0
51	153758.0
52	130321.0
53	115682.5
54	101044.0
55	88947.5
56	76851.0
57	75094.0
58	73337.0
59	73094.5
60	72852.0
61	65934.5
62	59017.0
63	48946.5
64	38876.0
65	32707.5
66	26539.0
67	22259.5
68	17980.0
69	15768.0
70	13556.0
71	11121.0
72	8686.0
73	7655.0
74	6624.0
75	5252.0
76	3880.0
77	2843.0
78	1806.0
79	1533.0
80	1260.0
81	991.5
82	723.0
83	468.0
84	213.0
85	165.0
86	117.0
87	72.0
88	27.0
89	30.0
90	33.0
91	18.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2402783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.237012866317357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.19289196565083	16.63338612228708
2	7.0487901520480065	2.8529291399793317
3	2.273128849576941	1.3800401332705718
4	1.1823330276826527	0.957075547739432
5	0.7296612535025198	0.7383082087591871
6	0.5118869569557633	0.6215437760408292
7	0.3847481941388208	0.5450307908555779
8	0.32054916009716217	0.5189565981738795
9	0.24830016328842142	0.45223682392588366
>10	2.945163084717632	13.97117177719468
>50	0.9945924543035412	14.518537144938906
>100	1.1531705011635112	44.00915480646855
>500	0.012493721302136562	1.6534183979570967
>1k	0.0020822868836894272	0.7335603504762631
>5k	2.082286883689427E-4	0.41465038193281417
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9840	0.40952512149453363	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4225	0.17583776812138258	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2409	0.10025874163418003	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04049470967623793	0.0
2	0.0	0.0	0.0	0.16888749420983917	0.0
3	0.0	0.0	0.0	0.24713009872302244	0.0
4	0.0	0.0	0.0	0.42971004872266866	0.0
5	0.0	0.0	0.0	0.649455235866077	0.0
6	0.0	0.0	0.0	1.0827028491545012	0.0
7	0.0	0.0	0.0	1.2825544379163663	0.0
8	0.0	0.0	0.0	1.8870201761873628	0.0
9	0.0	0.0	0.0	2.1574565826377166	0.0
10	0.0	0.0	0.0	2.6070602297419283	0.0
11	0.0	0.0	0.0	2.9815426528321534	0.0
12	0.0	0.0	0.0	3.3070818296949827	0.0
13	0.0	0.0	0.0	3.4756779950582306	0.0
14	0.0	0.0	0.0	3.5557101910576194	0.0
15	0.0	0.0	0.0	3.630456849411703	0.0
16	0.0	0.0	0.0	3.7672981704964617	0.0
17	0.0	0.0	0.0	3.908592661093407	0.0
18	0.0	0.0	0.0	4.134039569948681	0.0
19	0.0	0.0	0.0	4.22310296019241	0.0
20	0.0	0.0	0.0	4.320656505393954	0.0
21	0.0	0.0	0.0	4.452378762460031	0.0
22	0.0	0.0	0.0	4.590884819811027	0.0
23	0.0	0.0	0.0	4.743083332951831	0.0
24	0.0	0.0	0.0	4.867147803193214	0.0
25	0.0	0.0	0.0	4.96436840114151	0.0
26	0.0	0.0	0.0	5.071410943060609	0.0
27	0.0	0.0	0.0	5.170462750901767	0.0
28	0.0	0.0	0.0	5.263355034557844	0.0
29	0.0	0.0	0.0	5.369939774003728	0.0
30	0.0	0.0	0.0	5.523053892090963	0.0
31	0.0	0.0	0.0	5.6450790604062036	0.0
32	0.0	0.0	0.0	5.765772439708455	0.0
33	0.0	0.0	0.0	5.866863549475754	0.0
34	0.0	0.0	0.0	5.9812309309663005	0.0
35	0.0	0.0	0.0	6.124523105082731	0.0
36	0.0	0.0	0.0	6.250085837963728	0.0
37	0.0	0.0	0.0	6.3762728469445635	0.0
38	0.0	0.0	0.0	6.506371986151059	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCTA	20	7.858198E-4	44.000004	33
CCCGTTA	20	7.858198E-4	44.000004	14
TATCGCT	20	7.858198E-4	44.000004	33
GACACGC	25	4.4442942E-5	44.0	10
CGCGATC	30	2.5288737E-6	44.0	39
GTTCGCT	25	4.4442942E-5	44.0	25
ATACGAC	25	4.4442942E-5	44.0	18
TACACCG	30	2.5288737E-6	44.0	24
AAGTACG	110	0.0	42.0	1
AGTACGG	300	0.0	41.8	2
CCGGATA	85	0.0	41.411766	20
GCGATAC	240	0.0	41.25	9
CGGGTAT	385	0.0	41.14286	6
CGATACG	145	0.0	40.96552	10
GTCGTTT	370	0.0	40.43243	9
CACGACG	120	0.0	40.333332	26
ATGACGG	300	0.0	40.333332	2
CGGTCTA	115	0.0	40.173916	31
CGGGTCA	280	0.0	40.07143	6
GTTAGTC	55	7.8216544E-11	40.0	15
>>END_MODULE