FastQCFastQC Report
Thu 26 May 2016
SRR1546618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546618_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3982056
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222710.559283947789785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA207.858824E-444.021
CGGTCGA207.858824E-444.06
CGTTTTT237400.042.1095161
TATAGCG3000.039.61
TGCGGGA34500.037.878264
TAGGGAC48350.037.8572925
CGTAAGG6000.037.7666662
TACGGGA19150.037.6814654
GGGCGAT79350.037.6509177
AGGGATT136200.037.5389146
AGGGCGA40350.037.511776
TATTGCG3600.037.277781
GGCGATA17650.037.2691238
AAGGGAT108250.036.9274835
GCGCGAC1500.036.6666689
ATTCGCG601.9826984E-1036.6666641
CGGGATA16750.036.644786
TGGGCGA21500.036.632566
ACGGGCG5350.036.598135
TAGGGAT111600.036.5878145
Produced by FastQC (version 0.11.5)