##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546618_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3982056
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.957940571403316	31.0	31.0	33.0	30.0	34.0
2	31.33937970736725	31.0	31.0	34.0	30.0	34.0
3	31.459846873072603	31.0	31.0	34.0	30.0	34.0
4	35.25363128996679	37.0	35.0	37.0	33.0	37.0
5	35.04039973320315	37.0	35.0	37.0	32.0	37.0
6	35.12271951976567	37.0	35.0	37.0	32.0	37.0
7	35.705912473355475	37.0	35.0	37.0	35.0	37.0
8	35.76147648350501	37.0	35.0	37.0	35.0	37.0
9	37.51881239239227	39.0	37.0	39.0	35.0	39.0
10	36.74656283085923	39.0	37.0	39.0	32.0	39.0
11	36.323654413699856	38.0	35.0	39.0	31.0	39.0
12	36.04277187462959	38.0	35.0	39.0	31.0	39.0
13	35.97529040274672	38.0	35.0	39.0	31.0	39.0
14	36.96818578141543	39.0	35.0	41.0	31.0	41.0
15	37.113561938857714	39.0	35.0	41.0	31.0	41.0
16	37.1102867463441	39.0	35.0	41.0	31.0	41.0
17	37.0079391148693	39.0	35.0	41.0	31.0	41.0
18	36.892699650632736	38.0	35.0	40.0	31.0	41.0
19	36.83727627135329	38.0	35.0	40.0	31.0	41.0
20	36.68779243687181	38.0	35.0	40.0	31.0	41.0
21	36.59303636111596	38.0	35.0	40.0	31.0	41.0
22	36.51403747209984	38.0	35.0	40.0	30.0	41.0
23	36.31862359544918	38.0	35.0	40.0	30.0	41.0
24	36.27690042530793	38.0	35.0	40.0	30.0	41.0
25	36.1373403588498	38.0	34.0	40.0	30.0	41.0
26	36.025812293950665	38.0	34.0	40.0	30.0	41.0
27	35.84610387196965	38.0	34.0	40.0	29.0	41.0
28	35.786759653806975	38.0	34.0	40.0	29.0	41.0
29	35.673394849293935	38.0	34.0	40.0	29.0	41.0
30	35.61984889213009	38.0	34.0	40.0	28.0	41.0
31	35.52597627959024	38.0	34.0	40.0	27.0	41.0
32	35.27965352571636	38.0	34.0	40.0	26.0	41.0
33	35.00755890926697	38.0	34.0	40.0	25.0	41.0
34	34.863601114600094	38.0	34.0	40.0	24.0	41.0
35	34.61044696508537	38.0	33.0	40.0	24.0	41.0
36	34.4458422985513	38.0	33.0	40.0	23.0	41.0
37	34.51608289787989	38.0	33.0	40.0	23.0	41.0
38	34.461413651641266	38.0	33.0	40.0	23.0	41.0
39	34.37352463149689	38.0	33.0	40.0	23.0	41.0
40	34.407415917807285	38.0	33.0	40.0	23.0	41.0
41	34.42555679779491	38.0	33.0	40.0	23.0	41.0
42	34.185110405278074	38.0	33.0	40.0	21.0	41.0
43	34.262647486625	38.0	33.0	40.0	22.0	41.0
44	34.07906593980597	37.0	33.0	40.0	21.0	41.0
45	33.8423296909938	37.0	33.0	40.0	20.0	41.0
46	33.95725926506307	37.0	33.0	40.0	20.0	41.0
47	33.85086397579542	37.0	33.0	40.0	20.0	41.0
48	33.91563955906195	37.0	33.0	40.0	21.0	41.0
49	33.84598257784421	37.0	33.0	40.0	20.0	41.0
50	33.38566760487547	36.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	3.0
13	4.0
14	26.0
15	115.0
16	360.0
17	1098.0
18	2537.0
19	4888.0
20	8715.0
21	13690.0
22	20266.0
23	28846.0
24	41457.0
25	58414.0
26	77420.0
27	90058.0
28	96176.0
29	105532.0
30	121623.0
31	143417.0
32	173804.0
33	213794.0
34	292988.0
35	366564.0
36	333376.0
37	408601.0
38	569904.0
39	808376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.16037443973666	24.27821205929801	29.32261625652678	16.23879724443855
2	31.768111749307394	25.885673129659654	29.096024767105234	13.25019035392772
3	26.435740732927915	26.00990543578493	34.47093662168488	13.083417209602278
4	22.991690724590512	28.92033663012273	33.85926767478911	14.228704970497652
5	21.175116572946237	33.19312937839146	32.87992434059189	12.751829708070403
6	20.55716946220746	41.50371064595777	28.04350315515402	9.89561673668075
7	88.43956488808796	2.692629134296454	7.9487079036558	0.9190980739597836
8	89.25949308598372	2.030810214622798	7.507026520973086	1.202670178420394
9	83.27017500507276	5.6010010908937495	9.1007007435355	2.0281231604979943
10	34.85965541418805	42.677777509909454	13.854928207940823	8.607638867961676
11	23.39143397280199	26.07195880720914	35.93098640501289	14.605620814975984
12	24.55261804454784	24.575571011557848	34.191709006603624	16.680101937290686
13	20.38163200115719	28.98542863284695	34.75478496535458	15.878154400641275
14	17.740508923028706	31.42647918562672	33.13617387600777	17.6968380153368
15	16.440652768318678	26.763485998187868	40.03444451810823	16.76141671538522
16	19.031851887567626	25.65569143176289	38.76123289074789	16.551223789921586
17	19.474186199290013	24.739732439724605	33.854797622132885	21.931283738852493
18	20.054238313072442	25.7457203012715	35.74048180136091	18.45955958429515
19	22.576553418636	26.159451298525184	32.85388251697113	18.41011276586768
20	24.13886193463879	26.471275140279293	32.15522333186676	17.234639593215164
21	21.957099548574906	27.5650568450067	33.346341688816025	17.131501917602364
22	21.52594036849306	25.921760015429214	32.48005552910356	20.072244086974166
23	18.856439989794215	28.657984719451456	32.65335796382573	19.832217326928603
24	18.115239966489675	27.073627292032054	37.39294475014917	17.418187991329102
25	18.007355999011565	27.966532866438843	35.50424705227651	18.521864082273076
26	18.483140367689455	30.78007943635147	32.43746446559265	18.299315730366423
27	17.83751911073074	30.838265458848397	33.235067512862706	18.089147917558165
28	17.300007835148477	28.882491858477128	36.51533278286393	17.302167523510466
29	17.78852432009997	26.576220927078875	35.874633606358124	19.760621146463034
30	18.998753407787333	28.57692106791065	33.788424873984695	18.635900650317325
31	21.20191177622816	28.501658439760767	31.73528950873619	18.561140275274884
32	22.4117139487742	27.856815675118586	32.217301815946335	17.514168560160883
33	22.130226194709465	28.81144815642974	30.959760485538123	18.098565163322668
34	19.674811203056912	28.693795366011933	31.203378355301886	20.428015075629272
35	18.92846308540111	28.323961290348503	32.347837398569986	20.3997382256804
36	22.124174044764818	28.316678620290624	30.573954761058108	18.985192573886454
37	19.576319368687933	30.89203165399984	31.258801985707883	18.272846991604336
38	19.8152662845525	30.379582808478833	30.50989237720414	19.29525852976452
39	19.59839339275992	30.736634542557915	30.559364308286973	19.105607756395187
40	21.23360394730762	28.704995610307844	30.561272870095245	19.500127572289287
41	18.67351940806458	28.33686919520971	31.23082648762348	21.758784909102232
42	19.547213801111788	28.52757470010467	30.344324640336552	21.580886858446995
43	19.77792376601434	28.653037526343176	30.554919368286132	21.014119339356352
44	19.55853960868456	30.094503944695905	30.743892099960423	19.603064346659114
45	18.798705995093993	31.81595135779105	29.269729004313348	20.11561364280161
46	19.746809186008434	30.797181154659803	30.297037510271075	19.158972149060684
47	19.885832846147817	29.009612119970186	30.471670915727955	20.63288411815404
48	19.959940292150588	27.999807134806744	31.957385832846146	20.082866740196522
49	19.86436152580476	28.146942182631285	31.688127941947574	20.30056834961638
50	19.031450085081676	30.842383934329405	30.793364031043257	19.33280194954566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2367.0
1	3175.5
2	3984.0
3	24446.0
4	44908.0
5	35378.5
6	25849.0
7	26548.5
8	27248.0
9	28416.0
10	29584.0
11	29792.5
12	30001.0
13	30546.5
14	31092.0
15	30066.5
16	29041.0
17	28899.5
18	28758.0
19	28217.0
20	27676.0
21	31322.5
22	34969.0
23	38620.5
24	42272.0
25	49217.0
26	56162.0
27	71548.5
28	86935.0
29	97463.0
30	107991.0
31	123962.0
32	139933.0
33	155750.0
34	171567.0
35	186491.5
36	201416.0
37	217077.5
38	232739.0
39	249752.5
40	266766.0
41	287511.0
42	308256.0
43	315845.0
44	323434.0
45	327075.5
46	330717.0
47	316212.5
48	301708.0
49	280667.0
50	259626.0
51	236602.5
52	213579.0
53	188009.5
54	162440.0
55	142898.5
56	123357.0
57	112953.5
58	102550.0
59	91896.5
60	81243.0
61	70852.0
62	60461.0
63	49925.5
64	39390.0
65	30202.5
66	21015.0
67	17462.5
68	13910.0
69	10941.5
70	7973.0
71	6195.0
72	4417.0
73	3757.0
74	3097.0
75	2358.5
76	1620.0
77	1328.5
78	1037.0
79	815.0
80	593.0
81	410.0
82	227.0
83	149.0
84	71.0
85	58.0
86	45.0
87	31.5
88	18.0
89	14.0
90	10.0
91	6.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3982056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.555870095387064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.48653751049854	17.134014769084175
2	6.496467278247067	2.800740094576531
3	2.3335620156333423	1.5090587900556578
4	1.3013176533601722	1.1220413715466322
5	0.9336884988455638	1.0063233995335965
6	0.7230763326241434	0.9351923697056954
7	0.5510108211172637	0.8314262376809406
8	0.4515738257909503	0.7787253381781338
9	0.41262491389067857	0.8005040137752965
>10	5.096901898690173	25.766405345593547
>50	1.4462244909410156	22.143666939945344
>100	0.7647698555215308	24.24873073972377
>500	0.0018904461806483943	0.25114865213444043
>1k	2.363057725809489E-4	0.10480445223941363
>5k	0.0	0.0
>10k+	1.1815288629047445E-4	0.5672174862269699
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22271	0.559283947789785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023455220117446867	0.0
2	0.0	0.0	0.0	0.08505656374495989	0.0
3	0.0	0.0	0.0	0.13332308736994156	0.0
4	0.0	0.0	0.0	0.21609439947604955	0.0
5	0.0	0.0	0.0	0.3537619762253469	0.0
6	0.0	0.0	0.0	0.6382883615900932	0.0
7	0.0	0.0	0.0	0.7847955930303341	0.0
8	0.0	0.0	0.0	1.1525704309532563	0.0
9	0.0	0.0	0.0	1.3416436132490352	0.0
10	0.0	0.0	0.0	1.5966124032409388	0.0
11	0.0	0.0	0.0	1.783400333897866	0.0
12	0.0	0.0	0.0	1.9570543457952374	0.0
13	0.0	0.0	0.0	2.0632557653634205	0.0
14	0.0	0.0	0.0	2.1180515793851216	0.0
15	0.0	0.0	0.0	2.1657405119365474	0.0
16	0.0	0.0	0.0	2.2459754458500836	0.0
17	0.0	0.0	0.0	2.3421569159248388	0.0
18	0.0	0.0	0.0	2.4763840588881725	0.0
19	0.0	0.0	0.0	2.548407154495065	0.0
20	0.0	0.0	0.0	2.625503006487101	0.0
21	0.0	0.0	0.0	2.7173650998378727	0.0
22	0.0	0.0	0.0	2.8290159656217795	0.0
23	0.0	0.0	0.0	2.9484517545710056	0.0
24	0.0	0.0	0.0	3.047445842047425	0.0
25	0.0	0.0	0.0	3.1345867561882605	0.0
26	0.0	0.0	0.0	3.222481049990256	0.0
27	0.0	0.0	0.0	3.3141422420980517	0.0
28	0.0	0.0	0.0	3.410625064037271	0.0
29	0.0	0.0	0.0	3.516851596260826	0.0
30	0.0	0.0	0.0	3.6559003690555834	0.0
31	0.0	0.0	0.0	3.7791532816213533	0.0
32	0.0	0.0	0.0	3.890979935992864	0.0
33	0.0	0.0	0.0	4.003434406748674	0.0
34	0.0	0.0	0.0	4.122794857731785	0.0
35	0.0	0.0	0.0	4.265158501035646	0.0
36	0.0	0.0	0.0	4.397577532812195	0.0
37	0.0	0.0	0.0	4.537530361200345	0.0
38	0.0	0.0	0.0	4.678286794560398	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTCGA	20	7.858824E-4	44.0	21
CGGTCGA	20	7.858824E-4	44.0	6
CGTTTTT	23740	0.0	42.109516	1
TATAGCG	300	0.0	39.6	1
TGCGGGA	3450	0.0	37.87826	4
TAGGGAC	4835	0.0	37.857292	5
CGTAAGG	600	0.0	37.766666	2
TACGGGA	1915	0.0	37.681465	4
GGGCGAT	7935	0.0	37.650917	7
AGGGATT	13620	0.0	37.538914	6
AGGGCGA	4035	0.0	37.51177	6
TATTGCG	360	0.0	37.27778	1
GGCGATA	1765	0.0	37.269123	8
AAGGGAT	10825	0.0	36.927483	5
GCGCGAC	150	0.0	36.666668	9
ATTCGCG	60	1.9826984E-10	36.666664	1
CGGGATA	1675	0.0	36.64478	6
TGGGCGA	2150	0.0	36.63256	6
ACGGGCG	535	0.0	36.59813	5
TAGGGAT	11160	0.0	36.587814	5
>>END_MODULE