FastQCFastQC Report
Thu 26 May 2016
SRR1546617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546617_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2550813
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99650.39065976220130605No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28920.11337561789123703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAT254.444371E-544.015
TCGCGAA254.444371E-544.01
AATCGCG207.8582903E-444.01
TCGCAAG1550.041.1612931
CGTTTTT95150.041.0635831
CTAAGCG1450.040.965521
CGACGGT603.6379788E-1240.33333228
TATTGCG1650.038.6666681
TACGGGA11050.038.4253434
TTCGAAG2200.038.01
CGTTGCC357.293702E-637.71428712
CGTACCG357.293702E-637.71428730
CCGCAAC357.293702E-637.71428725
ACGACTC357.293702E-637.71428725
AAGGGAC31450.037.7042925
CATACGA2550.037.0980418
GCGATCG950.037.052639
AGGGACT41250.036.9066666
AGGGATT78900.036.833976
AACGGGA13200.036.8333324
Produced by FastQC (version 0.11.5)