##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546617_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2550813
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.071065185883874	31.0	31.0	33.0	30.0	34.0
2	31.454470006229386	31.0	31.0	34.0	30.0	34.0
3	31.610770762106043	31.0	31.0	34.0	30.0	34.0
4	35.3785032458279	37.0	35.0	37.0	33.0	37.0
5	35.19861942055337	37.0	35.0	37.0	32.0	37.0
6	35.26464778092318	37.0	35.0	37.0	32.0	37.0
7	35.779466389735354	37.0	35.0	37.0	35.0	37.0
8	35.82914466877815	37.0	35.0	37.0	35.0	37.0
9	37.61194489756795	39.0	37.0	39.0	35.0	39.0
10	36.89052901957141	39.0	37.0	39.0	32.0	39.0
11	36.466129034154996	38.0	35.0	39.0	32.0	39.0
12	36.019344028746914	37.0	35.0	39.0	31.0	39.0
13	35.86409117406882	38.0	35.0	39.0	31.0	39.0
14	36.84749450469321	39.0	35.0	41.0	31.0	41.0
15	37.0431928957552	39.0	35.0	41.0	32.0	41.0
16	37.09570125289466	39.0	35.0	41.0	32.0	41.0
17	37.01640771001245	38.0	35.0	41.0	32.0	41.0
18	36.938503136058976	38.0	35.0	40.0	31.0	41.0
19	36.912976372631	38.0	35.0	40.0	31.0	41.0
20	36.781040789740366	38.0	35.0	40.0	31.0	41.0
21	36.6808441073493	38.0	35.0	40.0	31.0	41.0
22	36.607429082414114	38.0	35.0	40.0	31.0	41.0
23	36.40853171126225	38.0	35.0	40.0	30.0	41.0
24	36.385592750232966	38.0	35.0	40.0	30.0	41.0
25	36.255784724321224	38.0	35.0	40.0	30.0	41.0
26	36.16634108419551	38.0	35.0	40.0	30.0	41.0
27	35.9952132908214	38.0	34.0	40.0	30.0	41.0
28	35.983747534609556	38.0	34.0	40.0	30.0	41.0
29	35.88705483310615	38.0	34.0	40.0	30.0	41.0
30	35.85089773338932	38.0	34.0	40.0	30.0	41.0
31	35.79007986865364	38.0	34.0	40.0	29.0	41.0
32	35.592764346112396	38.0	34.0	40.0	29.0	41.0
33	35.39315112475905	38.0	34.0	40.0	27.0	41.0
34	35.3151818655464	38.0	34.0	40.0	27.0	41.0
35	35.090855738935	38.0	34.0	40.0	26.0	41.0
36	34.903658559055486	38.0	34.0	40.0	25.0	41.0
37	34.96829285408221	38.0	34.0	40.0	25.0	41.0
38	34.94699062612587	38.0	34.0	40.0	25.0	41.0
39	34.85642969515994	38.0	34.0	40.0	24.0	41.0
40	34.857442313489855	38.0	34.0	40.0	24.0	41.0
41	34.89740643473277	38.0	34.0	40.0	24.0	41.0
42	34.678554249174674	38.0	33.0	40.0	24.0	41.0
43	34.74737975696376	38.0	33.0	40.0	24.0	41.0
44	34.5647548448279	37.0	33.0	40.0	24.0	41.0
45	34.40882455907195	37.0	33.0	40.0	23.0	41.0
46	34.49328116173157	37.0	33.0	40.0	23.0	41.0
47	34.378734152601545	37.0	33.0	40.0	23.0	41.0
48	34.43751031533868	37.0	33.0	40.0	23.0	41.0
49	34.36460140355251	37.0	33.0	40.0	24.0	41.0
50	33.87755354861372	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	3.0
14	17.0
15	45.0
16	173.0
17	481.0
18	1227.0
19	2280.0
20	4115.0
21	6594.0
22	10300.0
23	14852.0
24	21633.0
25	30338.0
26	39660.0
27	47127.0
28	53074.0
29	61406.0
30	73458.0
31	89693.0
32	110320.0
33	140144.0
34	206049.0
35	272838.0
36	208769.0
37	256839.0
38	364772.0
39	534599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.385326952622556	23.622350991625023	30.220600255683188	14.771721800069232
2	28.905529335157066	27.223398971229955	31.717965997507463	12.153105696105516
3	27.01962080324979	27.127076739847254	32.95459134009431	12.898711116808641
4	23.02199338014978	30.21758161025524	32.59835197640909	14.16207303318589
5	21.399569470596237	33.83866241860928	31.78704201366388	12.9747260971306
6	20.952300305824064	41.70537001340357	27.36276630235145	9.97956337842092
7	90.22919359435599	3.1193584163166803	5.700378663586864	0.95106932574046
8	91.1624254698404	2.293582477429745	5.287294678206517	1.2566973745233383
9	85.4795314278232	5.788507428807992	6.8613026513507664	1.8706584920180351
10	39.192563312167536	40.843605548505515	11.639661551042746	8.324169588284205
11	28.4927981784631	25.12990956216704	32.120621935045804	14.25667032432405
12	28.74115037048972	24.54260661208799	30.75482209005521	15.96142092736708
13	20.586220942107477	33.49712425019003	31.111923923862705	14.804730883839778
14	17.32580945761214	35.80834816193896	30.430964559142517	16.434877821306383
15	15.921041644369854	26.96591243654474	41.47720746287556	15.635838456209845
16	18.34740531744193	24.732271632612818	40.0525244304463	16.86779861949896
17	18.751433366538432	24.586827807448056	31.532691733968736	25.12904709204477
18	19.801529943590534	26.017234505234217	35.56995357950583	18.611281971669424
19	24.070757048831098	26.43992327152167	30.55025985832752	18.939059821319713
20	26.009433070946404	26.61445586171938	29.764118341877666	17.611992725456552
21	22.090996086345804	29.003145271723174	31.780651894121597	17.125206747809425
22	22.22981457284403	26.613475782034985	29.7117428835434	21.444966761577582
23	18.538050417651156	30.524581770596278	29.976442804705794	20.960925007046775
24	17.710392725770177	27.6790184149132	37.63357015978827	16.977018699528344
25	17.250264915538693	28.44563674404984	35.81799214603344	18.486106194378028
26	17.762924996853943	32.99802847170687	30.7844989028988	18.45454762854039
27	17.40002109131481	33.58156791579783	31.207971732933775	17.810439259953593
28	16.48878220394831	30.538773324426373	36.062188800198214	16.91025567142711
29	17.208474317795936	26.971871321025887	35.857038520659884	19.962615840518296
30	18.36218491908266	30.64195611359986	33.04107357144565	17.954785395871824
31	22.604440231408574	29.286427503701763	30.33722973812663	17.771902526763036
32	23.969142387152644	28.0185180175889	30.819820974724525	17.19251862053392
33	23.813427326895386	29.1440807303397	29.10256455490857	17.93992738785634
34	19.873428589238017	28.4926413657136	30.566372368339035	21.06755767670935
35	19.306236874282824	27.57336582493503	32.92824679817768	20.192150502604463
36	24.476392428610016	27.85578558679135	29.947118820548585	17.72070316405005
37	19.834578230548455	32.059072930865575	30.55037746788965	17.555971370696323
38	19.71528293136345	32.628656040250696	28.870873717516726	18.785187310869123
39	20.18199687707409	31.91409954394932	29.762275792070998	18.141627786905588
40	22.419322780619353	28.120132679267357	29.54242431726669	19.918120222846596
41	18.28330810608226	28.207202958429335	30.37337507688725	23.13611385860116
42	19.798903330036346	28.27384837696844	29.024511008843064	22.90273728415215
43	19.82101392771638	28.01593060722209	30.086838980356458	22.07621648470507
44	18.608890577239492	30.78567499852008	30.714011571996853	19.89142285224358
45	18.306908424882575	33.63892217892884	28.382205986875558	19.67196340931303
46	19.816113529294384	32.5272765976965	29.083354993094357	18.573254879914757
47	19.458110022177245	29.38631722513567	29.920382246758194	21.235190505928895
48	20.332889945284112	27.909925188557533	32.09176838913711	19.665416477021246
49	20.31371958665727	27.637071004420942	32.34227675646941	19.706932652452373
50	19.22998667483661	31.988820819087877	30.292420494955923	18.488772011119593
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2508.0
1	2822.0
2	3136.0
3	11419.5
4	19703.0
5	15423.5
6	11144.0
7	11927.5
8	12711.0
9	13601.0
10	14491.0
11	14871.0
12	15251.0
13	15157.5
14	15064.0
15	15081.5
16	15099.0
17	14691.0
18	14283.0
19	15024.0
20	15765.0
21	17096.5
22	18428.0
23	21256.5
24	24085.0
25	30268.5
26	36452.0
27	43755.5
28	51059.0
29	59307.0
30	67555.0
31	76637.5
32	85720.0
33	97970.0
34	110220.0
35	121343.0
36	132466.0
37	139226.0
38	145986.0
39	158265.0
40	170544.0
41	193798.5
42	217053.0
43	229564.5
44	242076.0
45	238049.5
46	234023.0
47	220087.0
48	206151.0
49	189548.0
50	172945.0
51	154657.5
52	136370.0
53	118923.5
54	101477.0
55	87480.0
56	73483.0
57	65398.0
58	57313.0
59	50469.5
60	43626.0
61	38623.5
62	33621.0
63	27706.0
64	21791.0
65	17292.5
66	12794.0
67	9553.0
68	6312.0
69	5226.0
70	4140.0
71	3289.0
72	2438.0
73	1871.5
74	1305.0
75	1152.0
76	999.0
77	828.0
78	657.0
79	467.5
80	278.0
81	240.0
82	202.0
83	133.5
84	65.0
85	38.0
86	11.0
87	8.5
88	6.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2550813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.865812520080155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59219435539686	15.811636111260958
2	6.3678013725528215	2.5300309646448684
3	2.1726404796985497	1.294838053296852
4	1.2654234556546162	1.0055466051418627
5	0.8872077464078016	0.8812551378250102
6	0.677602407941637	0.8076673439594059
7	0.5455954872495091	0.7587088363050372
8	0.4580535019537698	0.7279683995183808
9	0.3716553839659916	0.6644912561952114
>10	5.097856349825273	23.804614984569387
>50	1.6364697028163062	23.192340551915336
>100	0.9240986605215854	27.590484582914694
>500	0.002800902600884738	0.3466111986741824
>1k	4.0012894298353395E-4	0.18775270066928534
>5k	2.0006447149176698E-4	0.3960532731095318
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9965	0.39065976220130605	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2892	0.11337561789123703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03214661364827606	0.0
2	0.0	0.0	0.0	0.12693992072331448	0.0
3	0.0	0.0	0.0	0.18492143485233922	0.0
4	0.0	0.0	0.0	0.28053800886227254	0.0
5	0.0	0.0	0.0	0.4188076507372355	0.0
6	0.0	0.0	0.0	0.6696296435685407	0.0
7	0.0	0.0	0.0	0.786455141948861	0.0
8	0.0	0.0	0.0	1.083536895883783	0.0
9	0.0	0.0	0.0	1.237840641395508	0.0
10	0.0	0.0	0.0	1.4893290884122043	0.0
11	0.0	0.0	0.0	1.71635474650631	0.0
12	0.0	0.0	0.0	1.9184079742419378	0.0
13	0.0	0.0	0.0	2.031783592133175	0.0
14	0.0	0.0	0.0	2.0921957038795083	0.0
15	0.0	0.0	0.0	2.1475506044543446	0.0
16	0.0	0.0	0.0	2.2477147481998876	0.0
17	0.0	0.0	0.0	2.3706559438108554	0.0
18	0.0	0.0	0.0	2.530016900494078	0.0
19	0.0	0.0	0.0	2.6092465421808653	0.0
20	3.9203187375946414E-5	0.0	0.0	2.6949447097846844	0.0
21	3.9203187375946414E-5	0.0	0.0	2.7970298097116486	0.0
22	3.9203187375946414E-5	0.0	0.0	2.9127184156580666	0.0
23	3.9203187375946414E-5	0.0	0.0	3.040324790566772	0.0
24	3.9203187375946414E-5	0.0	0.0	3.14131220124721	0.0
25	3.9203187375946414E-5	0.0	0.0	3.2274808070995404	0.0
26	3.9203187375946414E-5	0.0	0.0	3.3114148312714415	0.0
27	3.9203187375946414E-5	0.0	0.0	3.4008373016759754	0.0
28	3.9203187375946414E-5	0.0	0.0	3.4953169832520063	0.0
29	3.9203187375946414E-5	0.0	0.0	3.5932073421297446	0.0
30	7.840637475189283E-5	0.0	0.0	3.735554115491806	0.0
31	7.840637475189283E-5	0.0	0.0	3.8519875819983667	0.0
32	7.840637475189283E-5	0.0	0.0	3.960070769593851	0.0
33	7.840637475189283E-5	0.0	0.0	4.067997144439832	0.0
34	7.840637475189283E-5	0.0	0.0	4.179138180650639	0.0
35	7.840637475189283E-5	0.0	0.0	4.307999057555375	0.0
36	1.1760956212783924E-4	0.0	0.0	4.431959536038118	0.0
37	1.1760956212783924E-4	0.0	0.0	4.551803679846386	0.0
38	1.1760956212783924E-4	0.0	0.0	4.674940891394234	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAAAT	25	4.444371E-5	44.0	15
TCGCGAA	25	4.444371E-5	44.0	1
AATCGCG	20	7.8582903E-4	44.0	1
TCGCAAG	155	0.0	41.161293	1
CGTTTTT	9515	0.0	41.063583	1
CTAAGCG	145	0.0	40.96552	1
CGACGGT	60	3.6379788E-12	40.333332	28
TATTGCG	165	0.0	38.666668	1
TACGGGA	1105	0.0	38.425343	4
TTCGAAG	220	0.0	38.0	1
CGTTGCC	35	7.293702E-6	37.714287	12
CGTACCG	35	7.293702E-6	37.714287	30
CCGCAAC	35	7.293702E-6	37.714287	25
ACGACTC	35	7.293702E-6	37.714287	25
AAGGGAC	3145	0.0	37.704292	5
CATACGA	255	0.0	37.09804	18
GCGATCG	95	0.0	37.05263	9
AGGGACT	4125	0.0	36.906666	6
AGGGATT	7890	0.0	36.83397	6
AACGGGA	1320	0.0	36.833332	4
>>END_MODULE