FastQCFastQC Report
Thu 26 May 2016
SRR1546616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546616_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1557553
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65800.42245753435035605No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26760.17180795773883778No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA207.857343E-444.00000428
GCGATCG351.4465331E-744.09
TATTACG800.041.2500041
CGTTTTT53950.040.737721
GTACGAG603.6379788E-1240.3333321
TAGTACG501.3478711E-939.61
ACGGGTA2850.039.368425
CGGGTAT2700.039.111116
AGGGCGA17700.038.406786
CGCATGG1900.038.2105262
TACGGGA3800.038.2105264
TAACGCG357.2918738E-637.7142871
CGTTAGG2450.037.7142872
GGGCGAT34850.037.6872337
AGTACGG1950.037.230772
TCGATAG651.0913936E-1137.2307661
CGTAAGG3500.037.0857122
GGCGATA8550.037.052638
AGGGATT14200.036.718316
CGAAACG301.3013078E-436.66666426
Produced by FastQC (version 0.11.5)