##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546616_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1557553
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.937300367948957	31.0	31.0	33.0	30.0	34.0
2	31.31316301917174	31.0	31.0	34.0	30.0	34.0
3	31.479296049636833	31.0	31.0	34.0	30.0	34.0
4	35.26483464768133	37.0	35.0	37.0	33.0	37.0
5	35.0616466983788	37.0	35.0	37.0	32.0	37.0
6	35.12485546238234	37.0	35.0	37.0	32.0	37.0
7	35.62235121373077	36.0	35.0	37.0	35.0	37.0
8	35.6555886059736	37.0	35.0	37.0	35.0	37.0
9	37.42079274348931	39.0	37.0	39.0	35.0	39.0
10	36.61891890677235	39.0	35.0	39.0	32.0	39.0
11	36.276683361657675	38.0	35.0	39.0	32.0	39.0
12	35.63249982504608	37.0	35.0	39.0	30.0	39.0
13	35.39539842303922	37.0	35.0	39.0	30.0	39.0
14	36.32353698397422	38.0	35.0	40.0	30.0	41.0
15	36.54769372213979	38.0	35.0	40.0	31.0	41.0
16	36.62288795309052	38.0	35.0	40.0	31.0	41.0
17	36.534565436938585	38.0	35.0	40.0	31.0	41.0
18	36.450920771235396	38.0	35.0	40.0	31.0	41.0
19	36.38935304288201	38.0	35.0	40.0	30.0	41.0
20	36.24995553923366	38.0	34.0	40.0	30.0	41.0
21	36.12031950116625	38.0	34.0	40.0	30.0	41.0
22	36.04701605659647	38.0	34.0	40.0	30.0	41.0
23	35.860705863620694	37.0	34.0	40.0	30.0	41.0
24	35.793904926509725	37.0	34.0	40.0	29.0	41.0
25	35.60588692648019	37.0	34.0	40.0	29.0	41.0
26	35.4669073861371	37.0	34.0	40.0	29.0	41.0
27	35.27161643937638	37.0	34.0	40.0	27.0	41.0
28	35.23975428123473	37.0	34.0	40.0	27.0	41.0
29	35.12233355783078	36.0	34.0	40.0	27.0	41.0
30	35.078117405956654	36.0	34.0	40.0	27.0	41.0
31	35.00773392622916	37.0	34.0	40.0	27.0	41.0
32	34.78955001852265	37.0	33.0	40.0	25.0	41.0
33	34.57349188117515	36.0	33.0	40.0	25.0	41.0
34	34.45236277674018	36.0	33.0	40.0	24.0	41.0
35	34.223418400529546	36.0	33.0	40.0	23.0	41.0
36	33.98608394064279	36.0	33.0	40.0	23.0	41.0
37	34.0315443519418	36.0	33.0	40.0	23.0	41.0
38	33.975634858011254	36.0	33.0	40.0	23.0	41.0
39	33.869716150911074	36.0	33.0	40.0	23.0	41.0
40	33.78343786696183	36.0	33.0	40.0	22.0	41.0
41	33.807167396550874	36.0	33.0	40.0	22.0	41.0
42	33.571168364736224	35.0	32.0	40.0	21.0	41.0
43	33.59770357734215	35.0	32.0	40.0	22.0	41.0
44	33.37365534270744	35.0	32.0	40.0	21.0	41.0
45	33.182936310995515	35.0	32.0	40.0	20.0	41.0
46	33.25193813629456	35.0	32.0	40.0	20.0	41.0
47	33.10798605248104	35.0	32.0	39.0	20.0	41.0
48	33.15465091717585	35.0	32.0	39.0	20.0	41.0
49	33.062377973654826	35.0	32.0	39.0	20.0	41.0
50	32.57173335353596	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	8.0
14	16.0
15	56.0
16	199.0
17	518.0
18	1214.0
19	2380.0
20	4023.0
21	6245.0
22	9296.0
23	13007.0
24	18198.0
25	24105.0
26	30308.0
27	36057.0
28	40683.0
29	46141.0
30	53414.0
31	63142.0
32	76401.0
33	94448.0
34	140177.0
35	177645.0
36	127001.0
37	150369.0
38	195828.0
39	246669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.047962412836036	23.494031984786393	29.946525094170152	14.511480508207425
2	30.9577908424304	27.00383229334732	28.66303746967198	13.375339394550299
3	28.378488565076115	26.115323202484923	32.192869199314565	13.313319033124394
4	24.560448344293903	29.610356758325402	31.221473683399537	14.607721213981161
5	22.67338575316538	33.26352297482012	30.418098132134187	13.644993139880313
6	21.47580210753663	41.626898089503214	26.899694585031774	9.99760521792838
7	87.91431174412685	4.415066453597405	6.360168803244577	1.3104529990311726
8	88.4473273140625	3.4690312303979383	6.1815552985997915	1.902086156939764
9	83.47895705635699	5.435834286216905	8.36164162632026	2.7235670311058437
10	43.86470315937885	26.212013331167544	16.92552356163803	12.99775994781558
11	37.337284830756964	25.071891614603164	22.906893055966634	14.68393049867324
12	34.155370635862795	22.89886764687943	26.535983045199746	16.409778672058028
13	22.302162430427728	32.645566475105504	28.047263881229085	17.00500721323769
14	18.94792665161314	34.37058000594522	28.57873857262	18.102754769821637
15	18.00221244477716	24.283796442239847	40.38109778607855	17.332893326904443
16	20.17286089141108	21.12255570115431	39.131252676473935	19.57333073096068
17	19.892099979904376	22.154815919586685	29.37055753479978	28.58252656570916
18	22.05343895199714	24.17253217065487	33.13062220033604	20.643406677011956
19	27.263855547772692	25.190410856002977	27.42943578805986	20.116297808164475
20	28.829837572140406	24.261389500068375	26.825796618156815	20.082976309634407
21	24.114813428499705	27.793725157346174	28.575078986076235	19.51638242807789
22	23.931127865311808	24.709913563133966	26.805765197075154	24.553193374479072
23	21.841568152094982	29.0026727822424	26.37701574200043	22.77874332366218
24	21.077677613538672	24.43846212616842	35.46376913016764	19.020091130125266
25	20.262167643733473	25.129353543667538	33.2801516224488	21.32832719015019
26	19.74667956724426	30.677928776741464	28.21233049533467	21.363061160679607
27	19.542320550247727	31.984144359774596	28.255410891314774	20.218124198662903
28	18.833516419665976	28.964728648078108	33.266283715546116	18.9354712167098
29	19.24981043983736	25.876422824777066	32.67163300382074	22.20213373156483
30	20.32296814297812	29.726693088453494	30.168090588249648	19.782248180318742
31	25.83725882843152	27.5957222643467	27.159268416548265	19.40775049067351
32	27.86235845585993	25.67636542705128	27.837062366417065	18.624213750671725
33	26.56551655064065	27.678865502490126	25.77684354882306	19.978774398046166
34	21.50167602643377	27.247612119780197	28.660148322400588	22.59056353138545
35	21.708988393974394	26.46272711105176	29.784797050244837	22.043487444729006
36	27.735364382464034	26.621630210978374	26.04739613997084	19.595609266586756
37	21.65428720563602	31.29036379500409	27.652542160684096	19.402806838675797
38	21.78391361321252	30.525574410630007	26.367577860913883	21.32293411524359
39	22.47114544416787	29.743771158991056	26.689878289855944	21.095205106985123
40	24.873054079058626	25.72002365248566	27.14135570346563	22.265566564990085
41	19.827575690843265	25.18476096800558	28.410204981788738	26.57745835936241
42	22.096262534886453	25.558488218378443	26.757420132733845	25.58782911400126
43	21.6278354572846	26.280646629681303	27.7821685682606	24.3093493447735
44	20.514550708707826	28.047777507410665	29.22333943050413	22.21433235337738
45	19.687740962907842	32.48525090317954	25.62853398889155	22.19847414502107
46	21.605492718385825	30.116085937364574	27.061101612593603	21.217319731656
47	21.35394429595654	27.39540805353012	27.778894201353022	23.471753449160317
48	22.393523687476446	26.003930524354544	30.825917320309486	20.77662846785952
49	22.294137021340525	25.752638915016053	30.527821525174424	21.425402538468997
50	21.244285106189004	29.784411830608654	27.985243519803177	20.986059543399165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2025.0
1	2616.5
2	3208.0
3	8038.0
4	12868.0
5	9836.5
6	6805.0
7	7428.5
8	8052.0
9	8778.0
10	9504.0
11	9936.0
12	10368.0
13	10418.0
14	10468.0
15	10208.0
16	9948.0
17	9573.5
18	9199.0
19	9579.5
20	9960.0
21	9986.0
22	10012.0
23	10580.5
24	11149.0
25	12336.5
26	13524.0
27	16095.5
28	18667.0
29	20344.0
30	22021.0
31	26501.5
32	30982.0
33	34863.5
34	38745.0
35	43905.5
36	49066.0
37	53257.5
38	57449.0
39	64754.0
40	72059.0
41	87487.5
42	102916.0
43	116044.5
44	129173.0
45	129724.5
46	130276.0
47	125175.5
48	120075.0
49	114059.5
50	108044.0
51	99308.5
52	90573.0
53	84241.0
54	77909.0
55	71777.5
56	65646.0
57	65514.0
58	65382.0
59	63371.5
60	61361.0
61	58005.0
62	54649.0
63	49155.0
64	43661.0
65	36645.5
66	29630.0
67	24865.5
68	20101.0
69	17639.0
70	15177.0
71	12917.5
72	10658.0
73	8680.0
74	6702.0
75	5660.5
76	4619.0
77	3535.0
78	2451.0
79	1943.0
80	1435.0
81	1020.5
82	606.0
83	431.5
84	257.0
85	178.5
86	100.0
87	69.5
88	39.0
89	29.5
90	20.0
91	14.5
92	9.0
93	6.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1557553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.761634271282986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92471645895151	18.991418817335976
2	5.660371573629814	2.689993583443164
3	2.243133203837518	1.5990153243407512
4	1.3092302099225206	1.2443779770037593
5	0.9587457690970391	1.1390683162211883
6	0.7730888688526737	1.1021912976526254
7	0.5930576906807759	0.9864413963409787
8	0.5426569796732479	1.031553334860381
9	0.44007291621536976	0.9411166519025917
>10	5.75374346154983	32.23975287861147
>50	1.3453634500358382	21.873899970335277
>100	0.45390879724150945	15.326598644273112
>500	0.0010917830356770265	0.14352715343433117
>1k	5.458915178385132E-4	0.26428971994798894
>5k	2.729457589192566E-4	0.4267549342963846
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6580	0.42245753435035605	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2676	0.17180795773883778	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02497507307937515	0.0
2	0.0	0.0	0.0	0.12493956866957336	0.0
3	0.0	0.0	0.0	0.18939965445798634	0.0
4	0.0	0.0	0.0	0.32512537294076027	0.0
5	0.0	0.0	0.0	0.5154238732165133	0.0
6	0.0	0.0	0.0	0.8579483330583293	0.0
7	0.0	0.0	0.0	1.0115225613510423	0.0
8	0.0	0.0	0.0	1.3902576669943174	0.0
9	0.0	0.0	0.0	1.5830600949052778	0.0
10	0.0	0.0	0.0	1.8991328063956732	0.0
11	0.0	0.0	0.0	2.1956877229859915	0.0
12	0.0	0.0	0.0	2.4299012617869185	0.0
13	0.0	0.0	0.0	2.5557396762742584	0.0
14	0.0	0.0	0.0	2.616540175518907	0.0
15	0.0	0.0	0.0	2.677597487854346	0.0
16	0.0	0.0	0.0	2.7982354372531786	0.0
17	0.0	0.0	0.0	2.9166262721075946	0.0
18	0.0	0.0	0.0	3.0844536269391796	0.0
19	0.0	0.0	0.0	3.161112334540141	0.0
20	0.0	0.0	0.0	3.251574745771091	0.0
21	0.0	0.0	0.0	3.358730007903423	0.0
22	0.0	0.0	0.0	3.4638949685821285	0.0
23	0.0	0.0	0.0	3.5835056656177993	0.0
24	0.0	0.0	0.0	3.6778202732106067	0.0
25	0.0	0.0	0.0	3.758074364082635	0.0
26	0.0	0.0	0.0	3.842052244771125	0.0
27	0.0	0.0	0.0	3.9307811676392395	0.0
28	0.0	0.0	0.0	4.011613087965546	0.0
29	6.42032726976225E-5	0.0	0.0	4.098159099561941	0.0
30	6.42032726976225E-5	0.0	0.0	4.222777651868026	0.0
31	6.42032726976225E-5	0.0	0.0	4.324860855457246	0.0
32	6.42032726976225E-5	0.0	0.0	4.421615187412563	0.0
33	6.42032726976225E-5	0.0	0.0	4.520552430639599	0.0
34	6.42032726976225E-5	0.0	0.0	4.621223162229471	0.0
35	6.42032726976225E-5	0.0	0.0	4.744236632718116	0.0
36	6.42032726976225E-5	0.0	0.0	4.846576649398126	0.0
37	6.42032726976225E-5	0.0	0.0	4.960280645345616	0.0
38	6.42032726976225E-5	0.0	0.0	5.0808543914717506	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	20	7.857343E-4	44.000004	28
GCGATCG	35	1.4465331E-7	44.0	9
TATTACG	80	0.0	41.250004	1
CGTTTTT	5395	0.0	40.73772	1
GTACGAG	60	3.6379788E-12	40.333332	1
TAGTACG	50	1.3478711E-9	39.6	1
ACGGGTA	285	0.0	39.36842	5
CGGGTAT	270	0.0	39.11111	6
AGGGCGA	1770	0.0	38.40678	6
CGCATGG	190	0.0	38.210526	2
TACGGGA	380	0.0	38.210526	4
TAACGCG	35	7.2918738E-6	37.714287	1
CGTTAGG	245	0.0	37.714287	2
GGGCGAT	3485	0.0	37.687233	7
AGTACGG	195	0.0	37.23077	2
TCGATAG	65	1.0913936E-11	37.230766	1
CGTAAGG	350	0.0	37.085712	2
GGCGATA	855	0.0	37.05263	8
AGGGATT	1420	0.0	36.71831	6
CGAAACG	30	1.3013078E-4	36.666664	26
>>END_MODULE