FastQCFastQC Report
Thu 26 May 2016
SRR1546615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546615_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2402465
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123260.513056381674655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACG302.5288755E-644.01
CGTTTTT139300.042.752331
TGCGGGC3700.039.2432444
TATCGCG452.3528628E-839.1111151
GCGTAAG1700.038.8235321
GCGAACG404.1270505E-738.51
TAACGCG404.1270505E-738.51
TAATCCG404.1270505E-738.51
CGTAAGG3950.038.430382
CGGGTAT1500.038.133336
TACGGGA12700.037.9370084
AGGGCGA24450.037.881396
CAACGCG357.293522E-637.7142871
CCACGCG357.293522E-637.71428737
ACGCGCC357.293522E-637.71428739
AGGGATC43050.037.5098726
ACGGGCC1350.037.481485
GTTAGCG2000.037.3999981
AGCGGGA21950.037.0842824
AAGGGAT68250.036.876195
Produced by FastQC (version 0.11.5)