##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546615_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2402465
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99272039342925	31.0	31.0	33.0	30.0	34.0
2	31.37498111314837	31.0	31.0	34.0	30.0	34.0
3	31.503273096590377	31.0	31.0	34.0	30.0	34.0
4	35.290797992894795	37.0	35.0	37.0	33.0	37.0
5	35.081474652076096	37.0	35.0	37.0	32.0	37.0
6	35.16433371557962	37.0	35.0	37.0	32.0	37.0
7	35.742648071876175	37.0	35.0	37.0	35.0	37.0
8	35.79133057089281	37.0	35.0	37.0	35.0	37.0
9	37.55161594445705	39.0	37.0	39.0	35.0	39.0
10	36.7933110367893	39.0	37.0	39.0	32.0	39.0
11	36.38804311405161	38.0	35.0	39.0	32.0	39.0
12	36.0825993302712	38.0	35.0	39.0	31.0	39.0
13	35.98956779807406	38.0	35.0	39.0	31.0	39.0
14	36.9873654767083	39.0	35.0	41.0	31.0	41.0
15	37.12498954199125	39.0	35.0	41.0	31.0	41.0
16	37.1384865128108	39.0	35.0	41.0	32.0	41.0
17	37.0288320537448	39.0	35.0	41.0	31.0	41.0
18	36.927192695835316	38.0	35.0	40.0	31.0	41.0
19	36.877089988823975	38.0	35.0	40.0	31.0	41.0
20	36.72404592782829	38.0	35.0	40.0	31.0	41.0
21	36.635082717125954	38.0	35.0	40.0	31.0	41.0
22	36.5762943476804	38.0	35.0	40.0	31.0	41.0
23	36.37745898483433	38.0	35.0	40.0	30.0	41.0
24	36.32593273991505	38.0	35.0	40.0	30.0	41.0
25	36.1922820935997	38.0	35.0	40.0	30.0	41.0
26	36.0879255264905	38.0	35.0	40.0	30.0	41.0
27	35.91888622727074	38.0	34.0	40.0	29.0	41.0
28	35.876880204290174	38.0	34.0	40.0	29.0	41.0
29	35.77027719446485	38.0	34.0	40.0	29.0	41.0
30	35.707650267537716	38.0	34.0	40.0	29.0	41.0
31	35.630144872037675	38.0	34.0	40.0	28.0	41.0
32	35.384139207022784	38.0	34.0	40.0	27.0	41.0
33	35.117248742437454	38.0	34.0	40.0	25.0	41.0
34	34.97657239543552	38.0	34.0	40.0	25.0	41.0
35	34.73526065936444	38.0	33.0	40.0	24.0	41.0
36	34.55908077745149	38.0	33.0	40.0	23.0	41.0
37	34.656630169430144	38.0	33.0	40.0	23.0	41.0
38	34.61671907811352	38.0	33.0	40.0	23.0	41.0
39	34.533201940506935	38.0	33.0	40.0	23.0	41.0
40	34.555356269498205	38.0	33.0	40.0	23.0	41.0
41	34.577684586455995	38.0	33.0	40.0	23.0	41.0
42	34.33663508105217	38.0	33.0	40.0	23.0	41.0
43	34.412038885061804	38.0	33.0	40.0	23.0	41.0
44	34.2119148457938	38.0	33.0	40.0	22.0	41.0
45	33.98933969901747	37.0	33.0	40.0	20.0	41.0
46	34.10187370055339	37.0	33.0	40.0	22.0	41.0
47	33.99211601417711	37.0	33.0	40.0	21.0	41.0
48	34.066112513605816	37.0	33.0	40.0	22.0	41.0
49	33.988225843040375	37.0	33.0	40.0	22.0	41.0
50	33.53172012911739	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	4.0
14	17.0
15	61.0
16	223.0
17	540.0
18	1373.0
19	2998.0
20	4899.0
21	7699.0
22	11589.0
23	16626.0
24	23870.0
25	33738.0
26	45226.0
27	52475.0
28	56344.0
29	62287.0
30	71122.0
31	85516.0
32	103346.0
33	127769.0
34	176282.0
35	225424.0
36	198857.0
37	245416.0
38	345870.0
39	502891.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.206947863964718	24.414674095148108	29.02572982332729	15.35264821755988
2	31.330862260220233	25.50467956869299	29.53358321557234	13.630874955514441
3	26.237843215197724	25.67063411953972	34.92696043438718	13.16456223087537
4	23.416907218211293	28.715048918506618	33.84802692234851	14.020016940933584
5	21.2207045680166	33.41967520858785	32.2355164383248	13.124103785070751
6	20.61178830909087	41.481353526482174	28.401329467859053	9.5055286965679
7	88.99742556083024	2.5796005352835527	7.503792979294182	0.9191809245920337
8	89.66836145375687	2.010018876445651	7.167554990395282	1.1540646794021974
9	83.68067797033464	5.686825822644659	8.610448019013804	2.0220481880069014
10	34.061973847693935	43.62073953210557	13.575390276237115	8.741896343963388
11	23.999766906073553	25.552963310599736	35.8719898104655	14.575279972861207
12	25.83933584880529	24.073024997242413	33.43836434661899	16.6492748073333
13	20.307517487247473	29.841308822396996	34.22526446795271	15.625909222402823
14	18.12992072725305	32.48605078533922	32.39310458216873	16.990923905238994
15	16.32348442120905	27.245558207923942	40.06751399083858	16.36344338002843
16	19.151912722974114	25.656148996967698	39.06171369822245	16.13022458183574
17	19.33988632508694	24.933765944561106	33.53081106280425	22.195536667547707
18	20.04815887016044	25.293063582612024	36.49822161821296	18.160555929014574
19	22.75983208912513	26.211911515880566	32.779957252238844	18.24829914275546
20	24.36784719028165	26.753147288305968	32.08800128201659	16.791004239395786
21	21.49042754004741	28.299350875038765	33.41905084985629	16.791170735057534
22	21.678359518244804	25.501224783711727	32.586156301964856	20.23425939607861
23	18.572382948346803	29.480096484235986	32.4408472131748	19.506673354242412
24	17.85753382463428	26.648837756221216	38.38311900485543	17.110509414289073
25	17.94028216852275	28.342556499262216	35.62848990515991	18.08867142705513
26	18.099951508138513	31.810619509545408	32.18236269831195	17.907066284004138
27	17.659195867577676	31.500812706948906	32.72372334248366	18.116268082989762
28	17.00490954082578	29.61387574845003	36.39953131471218	16.981683396012013
29	17.432303904531388	26.760473097422853	36.31774032087877	19.489482677166993
30	18.804519524738133	29.540784152942916	33.614683252409506	18.040013069909445
31	21.538253418884352	28.65943936748298	31.69731921172629	18.104988001906374
32	22.35437352885474	27.97892997400587	31.94989313059712	17.71680336654228
33	22.107626958145072	28.872262447111613	30.971231630845818	18.048878963897497
34	19.226211412028896	28.782687781091504	31.60791104136793	20.383189765511673
35	19.019174056645987	28.783020772414996	32.63610500048908	19.56170017044993
36	22.421263160961765	28.670344833327437	30.230159440408084	18.67823256530272
37	19.197657406039216	31.3450560153842	31.750639447400896	17.706647131175686
38	19.084440356050973	31.12794567246557	30.5768034081662	19.21081056331726
39	19.422634668975405	31.38801189611503	30.740468643663903	18.44888479124566
40	21.67856763782199	28.797089655832654	30.191782190375307	19.33256051597006
41	18.299996045728033	28.313752749779912	31.308343721968896	22.07790748252316
42	19.724949166793273	28.949100195008043	29.69824742504053	21.627703213158153
43	19.629130913457637	28.80429059320323	30.631663728711967	20.934914764627162
44	19.03157798344617	30.2150499591045	31.10921491051899	19.64415714693034
45	18.649179072327797	32.381949372831656	29.351686705113288	19.61718484972726
46	19.79142255974593	31.353672165879626	29.84971685331524	19.005188421059206
47	19.367274861444393	29.281092544532388	30.508956426004126	20.842676168019096
48	19.84482604325141	28.76120984072609	32.0644005219639	19.3295635940586
49	19.75200471182723	28.50114361707663	32.06839641784584	19.678455253250306
50	19.23262149500617	30.80040708189297	30.571933410060083	19.39503801304077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1610.0
1	1954.0
2	2298.0
3	13985.0
4	25672.0
5	20404.5
6	15137.0
7	15517.0
8	15897.0
9	16608.5
10	17320.0
11	17458.5
12	17597.0
13	17488.0
14	17379.0
15	17139.5
16	16900.0
17	16755.0
18	16610.0
19	16540.5
20	16471.0
21	18302.5
22	20134.0
23	22197.5
24	24261.0
25	29054.5
26	33848.0
27	42822.5
28	51797.0
29	58113.0
30	64429.0
31	77098.5
32	89768.0
33	98762.0
34	107756.0
35	116427.5
36	125099.0
37	134435.0
38	143771.0
39	151606.5
40	159442.0
41	175936.5
42	192431.0
43	201116.0
44	209801.0
45	207826.5
46	205852.0
47	196123.0
48	186394.0
49	172863.5
50	159333.0
51	142588.5
52	125844.0
53	111323.5
54	96803.0
55	84197.0
56	71591.0
57	63275.5
58	54960.0
59	47873.0
60	40786.0
61	35872.0
62	30958.0
63	25447.5
64	19937.0
65	15024.5
66	10112.0
67	7962.5
68	5813.0
69	4722.0
70	3631.0
71	2800.5
72	1970.0
73	1649.0
74	1328.0
75	1118.5
76	909.0
77	644.0
78	379.0
79	294.5
80	210.0
81	180.0
82	150.0
83	93.0
84	36.0
85	32.0
86	28.0
87	16.0
88	4.0
89	3.5
90	3.0
91	3.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2402465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.098009353998375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.34559808672051	18.638749461843894
2	6.069137594396799	2.925082690409545
3	2.5577738935778846	1.8491177763855813
4	1.6298473854071862	1.5710431015652868
5	1.1660160263114137	1.404933255448223
6	0.9145472500807392	1.3223260912271486
7	0.7455916982888566	1.257709300373988
8	0.6321490372063913	1.2186826729376548
9	0.5831613634943192	1.2647725193138897
>10	6.776259646657054	37.18416402406219
>50	1.281506276283872	21.282455896728962
>100	0.2977133196534951	9.433490874142361
>500	1.746054803759302E-4	0.023394503416433517
>1k	3.492109607518604E-4	0.10544357115392516
>5k	0.0	0.0
>10k+	1.746054803759302E-4	0.5186342609909343
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12326	0.513056381674655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023226144813764196	0.0
2	0.0	0.0	0.0	0.08362244611263847	0.0
3	0.0	0.0	0.0	0.1329884098207466	0.0
4	0.0	0.0	0.0	0.2071206032137825	0.0
5	0.0	0.0	0.0	0.3340735452961854	0.0
6	0.0	0.0	0.0	0.5930575471442873	0.0
7	0.0	0.0	0.0	0.716638952076305	0.0
8	0.0	0.0	0.0	1.0591205282907348	0.0
9	0.0	0.0	0.0	1.2252831987146535	0.0
10	0.0	0.0	0.0	1.468450112696751	0.0
11	0.0	0.0	0.0	1.6576724322726866	0.0
12	0.0	0.0	0.0	1.8141783543152554	0.0
13	0.0	0.0	0.0	1.9041692594897324	0.0
14	0.0	0.0	0.0	1.957406247333468	0.0
15	0.0	0.0	0.0	2.0063143479717707	0.0
16	0.0	0.0	0.0	2.092267733348873	0.0
17	0.0	0.0	0.0	2.19258136955169	0.0
18	0.0	0.0	0.0	2.335809262569902	0.0
19	0.0	0.0	0.0	2.404780090448768	0.0
20	0.0	0.0	0.0	2.4836574102016056	0.0
21	0.0	0.0	0.0	2.5773112199345256	0.0
22	0.0	0.0	0.0	2.683368956467628	0.0
23	0.0	0.0	0.0	2.8070336092305195	0.0
24	0.0	0.0	0.0	2.9061401518856673	0.0
25	0.0	0.0	0.0	2.987473282649279	0.0
26	0.0	0.0	0.0	3.0765484616841454	0.0
27	0.0	0.0	0.0	3.169161673531144	0.0
28	0.0	0.0	0.0	3.2658540290909546	0.0
29	0.0	0.0	0.0	3.37107928731532	0.0
30	0.0	0.0	0.0	3.5073976103710147	0.0
31	0.0	0.0	0.0	3.627524230321774	0.0
32	0.0	0.0	0.0	3.74702649154098	0.0
33	0.0	4.16239154368534E-5	0.0	3.8649054200581485	0.0
34	0.0	4.16239154368534E-5	0.0	3.990526396846572	0.0
35	0.0	4.16239154368534E-5	0.0	4.131256854938574	0.0
36	0.0	4.16239154368534E-5	0.0	4.2639538973512625	0.0
37	0.0	4.16239154368534E-5	0.0	4.410137088365492	0.0
38	0.0	4.16239154368534E-5	0.0	4.54653865925206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGACG	30	2.5288755E-6	44.0	1
CGTTTTT	13930	0.0	42.75233	1
TGCGGGC	370	0.0	39.243244	4
TATCGCG	45	2.3528628E-8	39.111115	1
GCGTAAG	170	0.0	38.823532	1
GCGAACG	40	4.1270505E-7	38.5	1
TAACGCG	40	4.1270505E-7	38.5	1
TAATCCG	40	4.1270505E-7	38.5	1
CGTAAGG	395	0.0	38.43038	2
CGGGTAT	150	0.0	38.13333	6
TACGGGA	1270	0.0	37.937008	4
AGGGCGA	2445	0.0	37.88139	6
CAACGCG	35	7.293522E-6	37.714287	1
CCACGCG	35	7.293522E-6	37.714287	37
ACGCGCC	35	7.293522E-6	37.714287	39
AGGGATC	4305	0.0	37.509872	6
ACGGGCC	135	0.0	37.48148	5
GTTAGCG	200	0.0	37.399998	1
AGCGGGA	2195	0.0	37.084282	4
AAGGGAT	6825	0.0	36.87619	5
>>END_MODULE