FastQCFastQC Report
Thu 26 May 2016
SRR1546614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546614_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2433679
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152970.6285545464295004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG254.4443117E-544.01
CGTTTTT142150.042.2047121
TACGGGA10550.039.6208534
ACGGGAT15550.038.7652745
CGTAAGG3950.038.430382
TAACGCG750.038.133331
AGGGCGA30850.037.938416
TCCGCGA357.2935636E-637.71428714
ACGGGAC7650.036.810465
TAGGGCG13050.036.7509575
TAGGGAT46850.036.721455
GGGCGAT56150.036.712387
TATACGG2650.036.5283052
CATACGA5000.036.5218
AAGGGCG12000.036.1166655
AACGGGA12150.036.032924
AAGGGAT46600.036.0214585
GGCACCG12750.035.8901988
TAGGGAC26650.035.8273935
CGGTAGT4500.035.6888912
Produced by FastQC (version 0.11.5)