##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546614_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2433679
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.958507264105084	31.0	31.0	33.0	30.0	34.0
2	31.33997499259352	31.0	31.0	34.0	30.0	34.0
3	31.467972974250095	31.0	31.0	34.0	30.0	34.0
4	35.26602234723643	37.0	35.0	37.0	33.0	37.0
5	35.06300625513882	37.0	35.0	37.0	32.0	37.0
6	35.13612025250659	37.0	35.0	37.0	32.0	37.0
7	35.67344789514147	37.0	35.0	37.0	35.0	37.0
8	35.713489330351294	37.0	35.0	37.0	35.0	37.0
9	37.47032291440243	39.0	37.0	39.0	35.0	39.0
10	36.7026974387337	39.0	37.0	39.0	32.0	39.0
11	36.32798573682067	38.0	35.0	39.0	32.0	39.0
12	36.028861242587865	38.0	35.0	39.0	31.0	39.0
13	35.96077790045442	38.0	35.0	39.0	30.0	39.0
14	36.93575118164721	39.0	35.0	41.0	31.0	41.0
15	37.049262454087	39.0	35.0	41.0	31.0	41.0
16	37.046252196776976	39.0	35.0	41.0	31.0	41.0
17	36.94603150210032	39.0	35.0	41.0	31.0	41.0
18	36.831449833770186	38.0	35.0	40.0	31.0	41.0
19	36.7652878625324	38.0	35.0	40.0	31.0	41.0
20	36.62103301215978	38.0	35.0	40.0	31.0	41.0
21	36.526248120643686	38.0	35.0	40.0	30.0	41.0
22	36.463909578872155	38.0	35.0	40.0	30.0	41.0
23	36.25114446071154	38.0	35.0	40.0	30.0	41.0
24	36.185826890070544	38.0	34.0	40.0	30.0	41.0
25	36.024145748062914	38.0	34.0	40.0	30.0	41.0
26	35.90385913672263	38.0	34.0	40.0	29.0	41.0
27	35.72069775841432	38.0	34.0	40.0	29.0	41.0
28	35.65997693204404	38.0	34.0	40.0	29.0	41.0
29	35.516930540141075	38.0	34.0	40.0	27.0	41.0
30	35.49091231834601	38.0	34.0	40.0	27.0	41.0
31	35.41073165359934	38.0	34.0	40.0	27.0	41.0
32	35.184055497869686	38.0	34.0	40.0	26.0	41.0
33	34.92692421638186	38.0	33.0	40.0	25.0	41.0
34	34.75166692074016	38.0	33.0	40.0	24.0	41.0
35	34.51742731888634	38.0	33.0	40.0	23.0	41.0
36	34.326509371203024	37.0	33.0	40.0	23.0	41.0
37	34.393976362535895	38.0	33.0	40.0	23.0	41.0
38	34.33573901899141	38.0	33.0	40.0	23.0	41.0
39	34.22887324088345	37.0	33.0	40.0	22.0	41.0
40	34.23499524793533	37.0	33.0	40.0	22.0	41.0
41	34.241943986861045	37.0	33.0	40.0	22.0	41.0
42	33.989222079000555	37.0	33.0	40.0	21.0	41.0
43	34.056464718642026	37.0	33.0	40.0	22.0	41.0
44	33.85939682267053	37.0	33.0	40.0	21.0	41.0
45	33.62509435303505	37.0	32.0	40.0	20.0	41.0
46	33.72722902239778	37.0	32.0	40.0	20.0	41.0
47	33.58835902351954	37.0	32.0	40.0	20.0	41.0
48	33.64069213729502	36.0	32.0	40.0	20.0	41.0
49	33.554357004354316	36.0	32.0	40.0	20.0	41.0
50	33.08752017007995	36.0	31.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	6.0
13	10.0
14	36.0
15	69.0
16	218.0
17	711.0
18	1563.0
19	3185.0
20	5470.0
21	8482.0
22	12592.0
23	18291.0
24	25581.0
25	36341.0
26	47592.0
27	56674.0
28	60997.0
29	66995.0
30	76422.0
31	91012.0
32	109852.0
33	133764.0
34	183509.0
35	223672.0
36	210716.0
37	256181.0
38	344407.0
39	459327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.808303395805282	23.679951217888636	28.620578145268954	15.89116724103713
2	33.2722187272849	25.449001285707766	28.057192423487237	13.221587563520087
3	27.34123933353577	25.664313165376367	33.574066259354666	13.420381241733196
4	24.54078783602932	28.326660993499964	32.95475697493384	14.17779419553688
5	22.063715058559488	32.28104445984865	31.763802868003545	13.891437613588318
6	20.72031685361956	40.893889457073016	28.69717000475412	9.688623684553303
7	87.79950026277089	3.029898355534974	8.067251268552672	1.103350113141462
8	88.35216148062256	2.5017678995463246	7.8043981971328185	1.3416724226983099
9	82.45388976935743	5.425736097488617	9.580762294452144	2.539611838701817
10	36.00676177918288	36.84417706690159	15.862034393196472	11.28702676071906
11	25.76095697090701	26.499509590213005	32.19623458968911	15.543298849190876
12	25.814867120930902	24.362087193915055	32.83156899492497	16.991476690229074
13	21.942376130952358	28.082503896364315	33.56124616270264	16.413873809980693
14	19.62288370816365	29.73966574885184	32.72892604160204	17.908524501382477
15	18.33795664917189	25.575969550626848	38.2045454638841	17.881528336317157
16	21.010905711065426	24.378646485423918	36.68832249446209	17.922125309048564
17	21.752786624694547	24.419202368101956	32.076867984643826	21.75114302255967
18	22.004257751330396	24.836060959559582	34.32852072931558	18.83116055979445
19	24.34852747630234	26.10257967464074	30.72106058358559	18.82783226547133
20	25.474518208851705	26.00079961243862	30.416008027352824	18.108674151356855
21	23.792989954714653	26.88271542795907	31.554983216767702	17.76931140055858
22	22.853712424687068	25.345865251744375	30.99295346674726	20.8074688568213
23	20.821809285448083	27.72440408122846	31.017936219197356	20.435850414126104
24	20.373968793748066	26.148929254844212	35.541458014799815	17.93564393660791
25	20.86376222994076	27.46089356895466	32.702751677604155	18.972592523500428
26	20.134208332323205	29.42783333381272	30.759931774075383	19.678026559788698
27	19.505160705253243	29.023096308099795	31.59212862501587	19.879614361631095
28	18.820518235971136	28.521551116642748	33.73522144867914	18.922709198706976
29	19.706707417042264	26.129699109866174	33.851999380361995	20.311594092729568
30	20.138687148140736	28.48387153770074	32.23025715388101	19.147184160277504
31	22.540976028473764	27.67431530616815	30.65552194845746	19.129186716900627
32	23.443313600520035	27.71466573857933	29.679756451035654	19.162264209864983
33	22.723991126192075	28.25836932479592	29.49575519203642	19.52188435697559
34	20.537835926595086	28.70444294420094	30.137376375438173	20.620344753765803
35	20.716700928922837	28.091009537412287	30.830729936035112	20.36155959762976
36	23.439985306196913	28.780623903152385	28.144138976422116	19.63525181422858
37	20.72471348933035	30.56076828538193	29.425819921197494	19.288698304090225
38	20.820371133580064	29.701246548949143	29.666977444437002	19.811404873033787
39	21.05643349020146	29.749568451714463	29.06624086414026	20.12775719394382
40	22.72481292725951	27.94415368666122	29.463951490726593	19.867081895352673
41	20.140618380649215	28.019677204758718	29.930529046764182	21.909175367827885
42	20.897168443332088	28.462217079573765	28.850887894418285	21.789726582675858
43	20.678610449447113	28.05600081193945	29.641707061613303	21.623681677000132
44	20.558915123974856	29.072198921879178	29.740528639972652	20.628357314173314
45	19.668904567940142	30.69365351798655	28.685377159436392	20.952064754636908
46	20.970719638867738	29.85364955690541	28.932574920521564	20.24305588370529
47	20.48409013678468	29.019151662976096	29.511122871997497	20.985635328241727
48	20.880773512036715	27.996502414656987	30.601981608913913	20.520742464392384
49	21.05778946196273	27.840113671523646	30.533648850156492	20.56844801635713
50	20.40043078811955	29.909532029491153	29.83105002755088	19.858987154838413
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1680.0
1	2178.0
2	2676.0
3	15910.5
4	29145.0
5	22262.5
6	15380.0
7	15866.5
8	16353.0
9	16876.5
10	17400.0
11	17646.0
12	17892.0
13	17779.0
14	17666.0
15	17414.5
16	17163.0
17	16505.5
18	15848.0
19	16069.0
20	16290.0
21	16984.5
22	17679.0
23	19723.0
24	21767.0
25	25374.5
26	28982.0
27	34154.0
28	39326.0
29	47346.0
30	55366.0
31	62497.0
32	69628.0
33	79453.5
34	89279.0
35	98364.5
36	107450.0
37	116946.5
38	126443.0
39	134672.0
40	142901.0
41	156097.5
42	169294.0
43	176091.5
44	182889.0
45	183039.5
46	183190.0
47	179945.5
48	176701.0
49	170145.5
50	163590.0
51	149174.0
52	134758.0
53	123777.0
54	112796.0
55	105471.5
56	98147.0
57	89771.5
58	81396.0
59	76104.0
60	70812.0
61	64138.5
62	57465.0
63	50638.0
64	43811.0
65	37582.5
66	31354.0
67	25963.0
68	20572.0
69	17984.0
70	15396.0
71	12694.0
72	9992.0
73	7930.0
74	5868.0
75	4955.5
76	4043.0
77	3341.5
78	2640.0
79	1995.5
80	1351.0
81	1058.0
82	765.0
83	543.5
84	322.0
85	219.0
86	116.0
87	91.0
88	66.0
89	43.0
90	20.0
91	13.5
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2433679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.84381452470035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46802171939063	18.997573486672987
2	6.191074091329975	3.0761979286735923
3	2.6524075917128775	1.9768776675726556
4	1.6576560099434339	1.6472999382715803
5	1.2432559085561126	1.5443609599452937
6	0.9773656100508464	1.4568893963354301
7	0.8358362760635711	1.453575299087772
8	0.6936344347667078	1.378602019623156
9	0.613412997505878	1.3715566863368858
>10	7.297159207890822	40.98690158580107
>50	1.2196593348344364	20.47913824093081
>100	0.14951124033878035	4.806025203600047
>500	5.027888080313207E-4	0.0802767265581306
>1k	3.3519253868754715E-4	0.10779898602645234
>5k	0.0	0.0
>10k+	1.6759626934377358E-4	0.6369258745641722
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15297	0.6285545464295004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020914837166281994	0.0
2	0.0	0.0	0.0	0.07252394420135112	0.0
3	0.0	0.0	0.0	0.11398380805356828	0.0
4	0.0	0.0	0.0	0.179029362541239	0.0
5	0.0	0.0	0.0	0.29675236545164746	0.0
6	0.0	0.0	0.0	0.5570167635090741	0.0
7	0.0	0.0	0.0	0.6948738925716991	0.0
8	0.0	0.0	0.0	1.0388387293476256	0.0
9	0.0	0.0	0.0	1.2209909359451268	0.0
10	0.0	0.0	0.0	1.480392442881744	0.0
11	0.0	0.0	0.0	1.6548608094987054	0.0
12	0.0	0.0	0.0	1.8108797421516971	0.0
13	0.0	0.0	0.0	1.9061675759210643	0.0
14	0.0	0.0	0.0	1.9624609490405267	0.0
15	0.0	0.0	0.0	2.008974889457484	0.0
16	0.0	0.0	0.0	2.0861830997432285	0.0
17	0.0	0.0	0.0	2.1704177091555623	0.0
18	0.0	0.0	0.0	2.295413651512792	0.0
19	0.0	0.0	0.0	2.358117072958266	0.0
20	0.0	0.0	0.0	2.4271072725696365	0.0
21	0.0	0.0	0.0	2.5116706024089455	0.0
22	0.0	0.0	0.0	2.5993978663578887	0.0
23	0.0	0.0	0.0	2.703520061602208	0.0
24	0.0	0.0	0.0	2.792192396778704	0.0
25	0.0	0.0	0.0	2.8722769108004793	0.0
26	4.1090053371870327E-5	0.0	0.0	2.949320760872736	0.0
27	4.1090053371870327E-5	0.0	0.0	3.0326513891108893	0.0
28	4.1090053371870327E-5	0.0	0.0	3.1198033923126265	0.0
29	4.1090053371870327E-5	0.0	0.0	3.2155021266157124	0.0
30	4.1090053371870327E-5	0.0	0.0	3.347606648206275	0.0
31	4.1090053371870327E-5	0.0	0.0	3.453865526225932	0.0
32	4.1090053371870327E-5	0.0	0.0	3.5609051152596543	0.0
33	4.1090053371870327E-5	0.0	0.0	3.670040297015342	0.0
34	4.1090053371870327E-5	0.0	0.0	3.78468154592286	0.0
35	4.1090053371870327E-5	0.0	0.0	3.9118963511621705	0.0
36	4.1090053371870327E-5	0.0	0.0	4.030030254606298	0.0
37	4.1090053371870327E-5	0.0	0.0	4.152026623067381	0.0
38	4.1090053371870327E-5	0.0	0.0	4.285035125832125	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCG	25	4.4443117E-5	44.0	1
CGTTTTT	14215	0.0	42.204712	1
TACGGGA	1055	0.0	39.620853	4
ACGGGAT	1555	0.0	38.765274	5
CGTAAGG	395	0.0	38.43038	2
TAACGCG	75	0.0	38.13333	1
AGGGCGA	3085	0.0	37.93841	6
TCCGCGA	35	7.2935636E-6	37.714287	14
ACGGGAC	765	0.0	36.81046	5
TAGGGCG	1305	0.0	36.750957	5
TAGGGAT	4685	0.0	36.72145	5
GGGCGAT	5615	0.0	36.71238	7
TATACGG	265	0.0	36.528305	2
CATACGA	500	0.0	36.52	18
AAGGGCG	1200	0.0	36.116665	5
AACGGGA	1215	0.0	36.03292	4
AAGGGAT	4660	0.0	36.021458	5
GGCACCG	1275	0.0	35.890198	8
TAGGGAC	2665	0.0	35.827393	5
CGGTAGT	450	0.0	35.68889	12
>>END_MODULE