FastQCFastQC Report
Thu 26 May 2016
SRR1546613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546613_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2751384
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107250.38980382236721595No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36570.13291492572465347No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA254.4444627E-544.09
TACGCGC408.3200575E-944.034
CGCTAAG254.4444627E-544.013
CTCACGA1450.042.48275824
CTATGCG1700.041.4117661
ATAGACG1400.040.8571431
CGTTTTT92400.040.5952381
TACGATA557.8216544E-1140.022
CTACGAT557.8216544E-1140.021
TATCGTG1000.039.6000021
CATACGA8250.039.46666718
GTACGGG8700.039.4482733
TACGGGA7400.039.2432444
CAATTCG452.3530447E-839.111111
ACGGGTA1300.038.9230775
TCGATAG1300.038.9230771
GCGCGAC4650.038.7956969
TGGGCGA14700.038.1632656
GGATGCC20250.037.9160468
CGTTAGG3550.037.802822
Produced by FastQC (version 0.11.5)