##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546613_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2751384
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97456480084205	31.0	31.0	33.0	30.0	34.0
2	31.340103017245138	31.0	31.0	34.0	30.0	34.0
3	31.495451743558878	31.0	31.0	34.0	30.0	34.0
4	35.270475149960895	37.0	35.0	37.0	33.0	37.0
5	35.06013337287707	37.0	35.0	37.0	32.0	37.0
6	35.130988258999835	37.0	35.0	37.0	32.0	37.0
7	35.65947864783687	37.0	35.0	37.0	35.0	37.0
8	35.69672281295522	37.0	35.0	37.0	35.0	37.0
9	37.46929872384226	39.0	37.0	39.0	35.0	39.0
10	36.736821541449686	39.0	37.0	39.0	32.0	39.0
11	36.30277089639251	38.0	35.0	39.0	32.0	39.0
12	35.54026519017338	37.0	35.0	39.0	30.0	39.0
13	35.23914982423391	37.0	35.0	39.0	30.0	39.0
14	36.13888901004004	38.0	35.0	40.0	30.0	41.0
15	36.45343761539647	38.0	35.0	40.0	31.0	41.0
16	36.571838754604954	38.0	35.0	40.0	31.0	41.0
17	36.48122544871963	38.0	35.0	40.0	31.0	41.0
18	36.42083729497591	38.0	35.0	40.0	31.0	41.0
19	36.34479774542557	37.0	35.0	40.0	31.0	41.0
20	36.177114136013	37.0	34.0	40.0	30.0	41.0
21	36.06750857023229	37.0	34.0	40.0	30.0	41.0
22	35.97164917728678	37.0	34.0	40.0	30.0	41.0
23	35.796230915059475	37.0	34.0	40.0	30.0	41.0
24	35.739628492424174	37.0	34.0	40.0	30.0	41.0
25	35.591187562332266	36.0	34.0	40.0	29.0	41.0
26	35.46818510247933	36.0	34.0	40.0	29.0	41.0
27	35.277280815763994	36.0	34.0	40.0	29.0	41.0
28	35.25061496323305	36.0	34.0	40.0	29.0	41.0
29	35.150797925698484	36.0	34.0	40.0	28.0	41.0
30	35.108283322138966	36.0	34.0	40.0	27.0	41.0
31	34.98192037171111	36.0	34.0	40.0	27.0	41.0
32	34.75487027619555	36.0	33.0	40.0	26.0	41.0
33	34.51988490156227	36.0	33.0	40.0	25.0	41.0
34	34.390329739505646	36.0	33.0	40.0	24.0	41.0
35	34.143062182523416	36.0	33.0	40.0	23.0	41.0
36	33.8596782564702	36.0	33.0	40.0	23.0	41.0
37	33.88373051526068	36.0	33.0	40.0	23.0	41.0
38	33.85876671522405	35.0	33.0	40.0	23.0	41.0
39	33.778399888928625	35.0	33.0	40.0	23.0	41.0
40	33.69786878167497	35.0	33.0	40.0	22.0	41.0
41	33.72192576536027	35.0	33.0	40.0	22.0	41.0
42	33.481570729494685	35.0	32.0	40.0	21.0	41.0
43	33.517387612924985	35.0	33.0	40.0	22.0	41.0
44	33.32177587715855	35.0	32.0	40.0	20.0	41.0
45	33.11422505909753	35.0	32.0	40.0	20.0	41.0
46	33.1984085100444	35.0	32.0	40.0	20.0	41.0
47	33.06387294539766	35.0	32.0	39.0	20.0	41.0
48	33.12184994897114	35.0	32.0	39.0	20.0	41.0
49	33.02714452072121	35.0	32.0	39.0	20.0	41.0
50	32.50133859904688	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	4.0
13	4.0
14	32.0
15	89.0
16	308.0
17	929.0
18	2191.0
19	4199.0
20	6957.0
21	11197.0
22	16289.0
23	23444.0
24	32473.0
25	43042.0
26	53316.0
27	62846.0
28	70880.0
29	81073.0
30	94381.0
31	111138.0
32	134896.0
33	169602.0
34	261043.0
35	348115.0
36	209538.0
37	247367.0
38	329101.0
39	436927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.309048827789944	25.39819959700282	28.715112103581326	13.577639471625917
2	29.51023921052096	27.341512489714265	30.426977840970217	12.721270458794557
3	28.229429261782435	26.775724508102105	31.59758870444838	13.397257525667083
4	24.33055509518119	29.436276433969233	31.73530121567909	14.497867255170489
5	22.52266495698165	34.25748641411013	29.55239981042268	13.667448818485534
6	20.848307615367393	42.47106183651573	26.523269743518167	10.157360804598705
7	88.35953105782399	4.569954611933484	5.992038915687523	1.078475414555002
8	88.41568461545172	3.9318393942830223	5.550479322406469	2.1019966678587942
9	83.548933918348	6.395835695780741	7.561358210994904	2.493872174876353
10	46.435539350377844	30.561637343242527	12.889185951506587	10.113637354873038
11	37.5972601425319	23.3182282080582	25.210657618129638	13.873854031280258
12	35.53273552510301	22.454953579725696	26.520507497317713	15.491803397853591
13	20.129760149800973	37.408918566074384	27.414893740750106	15.046427543374536
14	16.12490295792954	38.91292527687884	28.69650328707298	16.26566847811865
15	14.739054962884135	25.243949953914104	44.15247017501011	15.86452490819166
16	16.34839048275341	21.680834082047433	42.96408643795268	19.006688997246478
17	17.303182689148443	22.15859363869238	30.005117424539797	30.53310624761938
18	19.927970795788593	24.587553027857982	35.681896819927715	19.802579356425714
19	26.936516313244535	24.427706201678863	27.8239242504863	20.8118532345903
20	27.247668809588195	25.130879586419052	27.98627890545267	19.635172698540078
21	21.343440246799428	30.030668201894027	29.400657996121225	19.225233555185316
22	22.220089961997306	26.0698252225062	26.549692809146237	25.160392006350257
23	19.00105546881133	30.74554478764142	26.55223698327823	23.701162760269014
24	18.25096024400811	25.48212099801409	38.79069588250859	17.47622287546922
25	17.46393814894613	26.720188821335007	35.66190688031914	20.15396614939972
26	17.664600797271483	33.48903679021176	28.127844023226128	20.718518389290626
27	17.554510748045345	35.47283839696676	28.41166482032315	18.560986034664737
28	16.333234473995635	30.87744204371327	35.90040503252182	16.88891844976928
29	16.978327997836725	26.394207424336262	34.56551321080591	22.061951367021106
30	18.876427281688052	32.52890181813953	30.07384647144855	18.520824428723873
31	26.078002925073346	28.538582764165234	26.906095259694755	18.47731905106666
32	28.336902446187086	25.950030966233722	28.707588617219553	17.005477970359646
33	27.732879161905426	27.975593374098274	25.366361075008072	18.92516638898823
34	21.70122382044818	27.82261581807556	27.559112068689796	22.917048292786465
35	21.29906258086839	26.275612564440298	30.645595089598544	21.779729765092767
36	29.626071824216467	26.677882840054313	25.748168921531857	17.947876414197363
37	20.74981173111423	33.64117840330539	27.839698130104708	17.769311735475675
38	20.738072184762288	33.591094518249726	25.181290579577404	20.489542717410583
39	21.44673371655865	32.885922139548676	27.306802685484833	18.36054145840784
40	24.878025023042948	26.827662005739654	26.350665701334307	21.943647269883083
41	18.578758908243998	25.416699377476938	28.73422975491607	27.270311959362996
42	21.07353971673892	25.652326247444922	26.831550957627144	26.442583078189013
43	20.431317475132516	25.53856531839976	29.118763502295568	24.911353704172154
44	19.461987130840335	28.578489952692898	30.07602719213312	21.883495724333642
45	17.62131349168273	35.70810181348732	25.69935712354219	20.97122757128776
46	21.375860294310066	31.817914184279623	26.949382565283507	19.85684295612681
47	21.003066093282506	27.93059783730661	28.042214391011942	23.02412167839894
48	21.463343539106138	26.19074618446571	31.86247357693437	20.483436699493783
49	21.88662142398153	25.90038322531497	32.338379520997435	19.87461582970607
50	20.74763101042966	31.80632001930665	27.836681466491047	19.609367503772646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3932.0
1	4870.5
2	5809.0
3	13755.5
4	21702.0
5	17177.0
6	12652.0
7	14078.0
8	15504.0
9	17026.0
10	18548.0
11	19466.5
12	20385.0
13	20393.0
14	20401.0
15	20374.0
16	20347.0
17	19525.0
18	18703.0
19	18493.0
20	18283.0
21	18537.0
22	18791.0
23	20090.5
24	21390.0
25	26506.0
26	31622.0
27	34332.0
28	37042.0
29	43907.5
30	50773.0
31	57606.0
32	64439.0
33	70171.5
34	75904.0
35	85353.5
36	94803.0
37	98334.5
38	101866.0
39	123233.0
40	144600.0
41	173764.0
42	202928.0
43	232081.0
44	261234.0
45	268520.0
46	275806.0
47	246203.5
48	216601.0
49	204302.0
50	192003.0
51	174804.5
52	157606.0
53	139059.0
54	120512.0
55	108822.5
56	97133.0
57	93676.0
58	90219.0
59	85714.5
60	81210.0
61	76801.0
62	72392.0
63	62671.0
64	52950.0
65	45227.5
66	37505.0
67	31527.5
68	25550.0
69	21655.0
70	17760.0
71	15690.5
72	13621.0
73	11323.0
74	9025.0
75	6901.0
76	4777.0
77	3563.0
78	2349.0
79	2051.0
80	1753.0
81	1109.5
82	466.0
83	355.0
84	244.0
85	178.5
86	113.0
87	106.0
88	99.0
89	59.5
90	20.0
91	14.5
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2751384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.511396206184067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8343922885568	16.990490397290667
2	6.7813134893064255	2.781884155550093
3	2.1466873902365715	1.3209466677588477
4	1.1409250234051764	0.936078607864536
5	0.7018718338536022	0.7198185635066113
6	0.4773826510081727	0.5875070818072274
7	0.38110447769610856	0.5471889456583005
8	0.29356101043337063	0.4817076956549279
9	0.2423278835389689	0.44734349079664393
>10	2.8722227060482055	13.672551666549424
>50	0.9622673055985866	14.25876787393325
>100	1.1481583618129225	44.377166153846524
>500	0.016887316964617784	2.1675802983644994
>1k	7.18609232536927E-4	0.3157607666505515
>5k	0.0	0.0
>10k+	1.7965230813423176E-4	0.3952076347680202
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10725	0.38980382236721595	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3657	0.13291492572465347	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044741119378465526	0.0
2	0.0	0.0	0.0	0.15177815964619987	0.0
3	0.0	0.0	0.0	0.2050967803839813	0.0
4	0.0	0.0	0.0	0.3288526792334331	0.0
5	0.0	0.0	0.0	0.5008751959014082	0.0
6	0.0	0.0	0.0	0.8394684275259288	0.0
7	0.0	0.0	0.0	1.0058574157587599	0.0
8	0.0	0.0	0.0	1.527849256955772	0.0
9	0.0	0.0	0.0	1.8045827118279383	0.0
10	0.0	0.0	0.0	2.259081247837452	0.0
11	0.0	0.0	0.0	2.612794142875004	0.0
12	0.0	0.0	0.0	2.9100263721821453	0.0
13	0.0	0.0	0.0	3.05628003942743	0.0
14	0.0	0.0	0.0	3.1268990442628146	0.0
15	0.0	0.0	0.0	3.1904670522180836	0.0
16	0.0	0.0	0.0	3.314986203307136	0.0
17	0.0	0.0	0.0	3.449500324200475	0.0
18	0.0	0.0	0.0	3.637660173934282	0.0
19	0.0	0.0	0.0	3.7190010554688113	0.0
20	0.0	0.0	0.0	3.8119724473210574	0.0
21	0.0	0.0	0.0	3.922425949994621	0.0
22	0.0	0.0	0.0	4.038767398516528	0.0
23	0.0	0.0	0.0	4.169792366314553	0.0
24	0.0	0.0	0.0	4.270977806078686	0.0
25	0.0	0.0	0.0	4.356062258121731	0.0
26	0.0	0.0	0.0	4.441728235680661	0.0
27	0.0	0.0	0.0	4.530919711679649	0.0
28	0.0	0.0	0.0	4.624181866289838	0.0
29	0.0	0.0	0.0	4.7231502400246566	0.0
30	0.0	0.0	0.0	4.857700706262739	0.0
31	0.0	0.0	0.0	4.970734728413046	0.0
32	0.0	0.0	0.0	5.078535020920381	0.0
33	0.0	0.0	0.0	5.1833186498140575	0.0
34	0.0	0.0	0.0	5.287557098536591	0.0
35	0.0	0.0	0.0	5.419345318574216	0.0
36	0.0	0.0	0.0	5.539357646915152	0.0
37	0.0	0.0	0.0	5.661877804043347	0.0
38	0.0	0.0	0.0	5.793556988046743	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTA	25	4.4444627E-5	44.0	9
TACGCGC	40	8.3200575E-9	44.0	34
CGCTAAG	25	4.4444627E-5	44.0	13
CTCACGA	145	0.0	42.482758	24
CTATGCG	170	0.0	41.411766	1
ATAGACG	140	0.0	40.857143	1
CGTTTTT	9240	0.0	40.595238	1
TACGATA	55	7.8216544E-11	40.0	22
CTACGAT	55	7.8216544E-11	40.0	21
TATCGTG	100	0.0	39.600002	1
CATACGA	825	0.0	39.466667	18
GTACGGG	870	0.0	39.448273	3
TACGGGA	740	0.0	39.243244	4
CAATTCG	45	2.3530447E-8	39.11111	1
ACGGGTA	130	0.0	38.923077	5
TCGATAG	130	0.0	38.923077	1
GCGCGAC	465	0.0	38.795696	9
TGGGCGA	1470	0.0	38.163265	6
GGATGCC	2025	0.0	37.916046	8
CGTTAGG	355	0.0	37.80282	2
>>END_MODULE