FastQCFastQC Report
Thu 26 May 2016
SRR1546612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546612_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1730469
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55770.3222825719501476No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18820.10875664343019147No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAG302.5284626E-644.036
CCCGTAG207.8575866E-444.035
TGTTACG254.4437766E-544.01
TATTGCG1400.042.4285741
ACGGGTA1050.041.904765
CGTTTTT55850.041.675921
CAACGCG750.041.0666661
AGTTGCG557.8216544E-1140.0000041
ACGATTG452.3521352E-839.111111
CATAGCG900.039.111111
TATCGCG850.038.823531
AGGGCGA16400.038.6341486
TAGCGCG404.1261592E-738.51
AGTAACG404.1261592E-738.51
CGACAGG1150.038.260872
CGACCCG750.038.1333332
TATAGCG750.038.133331
TAGGGAC19400.037.9896935
CTCCGAC357.2923394E-637.71428717
CGCGATT357.2923394E-637.71428729
Produced by FastQC (version 0.11.5)