##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546612_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1730469
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.931994736686992	31.0	31.0	33.0	30.0	34.0
2	31.30370321571782	31.0	31.0	34.0	30.0	34.0
3	31.46216083616638	31.0	31.0	34.0	30.0	34.0
4	35.23433936117896	37.0	35.0	37.0	33.0	37.0
5	35.02353639389091	37.0	35.0	37.0	32.0	37.0
6	35.08965546334549	37.0	35.0	37.0	32.0	37.0
7	35.66075844178659	37.0	35.0	37.0	35.0	37.0
8	35.70739377590699	37.0	35.0	37.0	35.0	37.0
9	37.45815556360732	39.0	37.0	39.0	35.0	39.0
10	36.70230440418176	39.0	37.0	39.0	32.0	39.0
11	36.25370983242115	38.0	35.0	39.0	31.0	39.0
12	35.721558721941854	37.0	35.0	39.0	30.0	39.0
13	35.50076019853577	37.0	35.0	39.0	30.0	39.0
14	36.42765805108326	38.0	35.0	40.0	30.0	41.0
15	36.67592831769884	38.0	35.0	40.0	31.0	41.0
16	36.754960071518184	38.0	35.0	40.0	31.0	41.0
17	36.68154991508082	38.0	35.0	40.0	31.0	41.0
18	36.61689981155398	38.0	35.0	40.0	31.0	41.0
19	36.57531513133145	38.0	35.0	40.0	31.0	41.0
20	36.43951553018286	38.0	35.0	40.0	31.0	41.0
21	36.310155801693064	38.0	34.0	40.0	30.0	41.0
22	36.239597473286146	38.0	34.0	40.0	30.0	41.0
23	36.07248670736084	38.0	34.0	40.0	30.0	41.0
24	36.02946946752586	38.0	34.0	40.0	30.0	41.0
25	35.88897691897399	38.0	34.0	40.0	30.0	41.0
26	35.76459156448339	37.0	34.0	40.0	29.0	41.0
27	35.56820838743716	37.0	34.0	40.0	29.0	41.0
28	35.54809071991466	37.0	34.0	40.0	29.0	41.0
29	35.474720437060704	37.0	34.0	40.0	29.0	41.0
30	35.437690013516566	37.0	34.0	40.0	29.0	41.0
31	35.385853777213	37.0	34.0	40.0	28.0	41.0
32	35.17445964071012	37.0	34.0	40.0	27.0	41.0
33	34.9719630920866	37.0	34.0	40.0	26.0	41.0
34	34.92213151463563	37.0	34.0	40.0	25.0	41.0
35	34.71789150802471	37.0	33.0	40.0	25.0	41.0
36	34.48460677423288	37.0	33.0	40.0	24.0	41.0
37	34.54797687794465	37.0	33.0	40.0	24.0	41.0
38	34.51905350514803	37.0	33.0	40.0	24.0	41.0
39	34.42478426368805	37.0	33.0	40.0	24.0	41.0
40	34.392186742438035	37.0	33.0	40.0	23.0	41.0
41	34.415949086634896	37.0	33.0	40.0	23.0	41.0
42	34.174864733202384	37.0	33.0	40.0	23.0	41.0
43	34.24700471375101	37.0	33.0	40.0	23.0	41.0
44	34.05918684472244	36.0	33.0	40.0	23.0	41.0
45	33.875073173804324	36.0	33.0	40.0	23.0	41.0
46	33.965717386442634	36.0	33.0	40.0	23.0	41.0
47	33.81824002625878	36.0	33.0	40.0	23.0	41.0
48	33.8801521437252	36.0	33.0	40.0	23.0	41.0
49	33.80065462022146	36.0	33.0	40.0	23.0	41.0
50	33.269983455352275	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	1.0
14	14.0
15	51.0
16	165.0
17	490.0
18	1071.0
19	2140.0
20	3600.0
21	5733.0
22	8501.0
23	12277.0
24	17092.0
25	23411.0
26	29934.0
27	35756.0
28	41206.0
29	47688.0
30	56087.0
31	66990.0
32	81881.0
33	100908.0
34	148808.0
35	195281.0
36	137925.0
37	166059.0
38	228545.0
39	318850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.22600347073539	23.51998215512673	29.612723487100894	14.64129088703698
2	29.363715848131346	26.861850746820657	30.659260581957838	13.115172823090157
3	27.63863438177743	26.816082807608804	32.363191712766884	13.182091097846884
4	23.61250042618504	30.19395319997064	31.916896517649263	14.276649856195053
5	22.34527171535578	33.26161867100769	30.575294905600735	13.817814708035797
6	20.21596457376584	42.11569233543045	27.313751358735693	10.354591732068013
7	89.03048826647574	3.554296551975216	6.06922169654585	1.3459934850031985
8	89.80166648463509	2.688982004300568	5.393219988338421	2.11613152272592
9	83.90372783332148	5.609577519158101	7.888959582633379	2.5977350648870337
10	41.94487159261448	36.55615905283481	11.985074566490356	9.513894788060346
11	31.29885597488311	24.52485424471632	30.47185473995778	13.704435040442794
12	31.78340669494802	23.01191179963351	29.25495920470115	15.94972230071732
13	21.248921535144518	33.906414966116124	29.5447072441055	15.299956254633859
14	16.61156599742613	36.83215359535478	29.710153721332194	16.84612668588689
15	15.1712050316995	26.683748740948264	42.50125255060912	15.643793676743126
16	17.33928778845504	23.505592992419974	42.240398412222355	16.914720806902636
17	17.441283259047115	23.80192884125633	31.422637446842444	27.33415045285411
18	19.234901058614746	25.6943637823041	35.48644904936176	19.584286109719386
19	24.699835709278815	26.60567742039875	29.251954238995324	19.442532631327115
20	26.915362251505226	25.82652448555854	28.71891955302291	18.539193709913324
21	21.226962170371152	30.43175000534537	29.94916407054966	18.392123753733813
22	21.55895309306321	26.289924870078572	28.383634725614847	23.767487311243368
23	18.133638915230495	31.37091736402097	28.582944854834153	21.912498865914383
24	18.132194220179613	26.19983368670574	38.24333172105366	17.424640372060985
25	17.751892694986157	27.12640330453767	35.65253119241084	19.469172808065327
26	18.01176444073832	33.33801414529818	29.600588048673508	19.04963336528999
27	18.018063311160155	33.80129895421415	29.737834078507042	18.44280365611866
28	16.26073625127061	30.1643080575266	36.020812854780985	17.5541428364218
29	16.983892805938737	26.356207479012916	35.38439579096765	21.275503924080695
30	18.392470480546024	31.349651452872024	30.874635720142923	19.38324234643903
31	23.78037399109721	28.527757503890566	27.527161711651583	20.164706793360644
32	24.832516502751567	27.539932815901352	28.79375475665846	18.833795924688626
33	24.661753547737636	28.308048280552846	27.76877251196063	19.261425659748888
34	18.97890109559894	28.010094373259502	29.648320773154563	23.362683757986996
35	19.37151142262589	26.45161514017298	32.41144452746625	21.765428909734872
36	25.06280089386172	26.815967232004734	28.890433749463295	19.230798124670248
37	19.90090547707009	31.51596474712925	30.369281391345353	18.21384838445531
38	19.23218503191909	31.58484780715517	28.934063539999848	20.248903620925887
39	19.503672125880325	31.962953395871292	29.585100917728084	18.9482735605203
40	23.3250638988621	27.041455235545968	29.184342510614176	20.449138354977755
41	18.05244705337108	26.25109146711094	30.08155592501224	25.614905554505746
42	19.790530775182912	27.469662848626587	28.29747311278041	24.44233326341009
43	20.07606030503869	27.32265068024911	29.39648153188529	23.20480748282691
44	19.339208041288227	28.952266697640926	30.59632966554154	21.112195595529304
45	18.263372530799455	33.471735119207565	27.336577540539587	20.92831480945339
46	20.25751400342913	32.2892811139639	27.838291237808942	19.61491364479803
47	20.1350616509166	28.15179006384974	28.826289289204254	22.8868589960294
48	20.603720725421837	26.401513115808488	32.015829234733474	20.9789369240362
49	20.735823640874237	26.34395646498146	32.15197729632834	20.768242597815966
50	19.004790030910694	31.57577512223565	29.650401134027828	19.769033712825827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1542.0
1	1950.5
2	2359.0
3	6792.0
4	11225.0
5	8986.5
6	6748.0
7	7243.5
8	7739.0
9	8474.0
10	9209.0
11	9322.5
12	9436.0
13	9643.5
14	9851.0
15	9687.0
16	9523.0
17	9517.5
18	9512.0
19	10346.5
20	11181.0
21	12028.0
22	12875.0
23	14702.5
24	16530.0
25	17975.0
26	19420.0
27	24896.5
28	30373.0
29	34339.5
30	38306.0
31	45397.5
32	52489.0
33	57443.5
34	62398.0
35	69307.0
36	76216.0
37	78342.5
38	80469.0
39	92906.0
40	105343.0
41	124243.0
42	143143.0
43	156411.0
44	169679.0
45	166209.0
46	162739.0
47	155951.0
48	149163.0
49	139271.0
50	129379.0
51	114345.5
52	99312.0
53	86628.5
54	73945.0
55	63458.5
56	52972.0
57	48277.0
58	43582.0
59	40814.0
60	38046.0
61	34567.0
62	31088.0
63	25374.5
64	19661.0
65	16968.5
66	14276.0
67	11221.5
68	8167.0
69	6506.0
70	4845.0
71	4037.0
72	3229.0
73	2704.0
74	2179.0
75	1675.0
76	1171.0
77	834.0
78	497.0
79	375.0
80	253.0
81	211.5
82	170.0
83	148.5
84	127.0
85	99.5
86	72.0
87	45.0
88	18.0
89	12.5
90	7.0
91	4.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1730469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.752435167022437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00262853253611	18.202546188779074
2	6.081629381951758	2.7674375644543217
3	2.1947084692330363	1.4980488647021915
4	1.3217751274581964	1.2029441157150174
5	0.9201133832579814	1.0467410049443444
6	0.7155648114123033	0.9768505187676642
7	0.5709255316771712	0.9092962301277856
8	0.46623324939500893	0.8486353423656114
9	0.41898649652085784	0.8579666788153821
>10	5.251689108038062	28.02626464882223
>50	1.4382105905664786	22.896753275642908
>100	0.6142014478870863	19.862690003332677
>500	0.0023080638917879204	0.34163322624101067
>1k	7.693546305959735E-4	0.23678012503604123
>5k	2.5645154353199116E-4	0.32541221225382005
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5577	0.3222825719501476	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1882	0.10875664343019147	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047328209866804895	0.0
2	0.0	0.0	0.0	0.1665444454653623	0.0
3	0.0	0.0	0.0	0.24559815864947596	0.0
4	0.0	0.0	0.0	0.4460062561074483	0.0
5	0.0	0.0	0.0	0.7438445877967187	0.0
6	0.0	0.0	0.0	1.3809551052344768	0.0
7	0.0	0.0	0.0	1.6984990774177404	0.0
8	0.0	0.0	0.0	2.594210008962888	0.0
9	0.0	0.0	0.0	3.0017295889149125	0.0
10	0.0	0.0	0.0	3.542103325745795	0.0
11	0.0	0.0	0.0	3.886171899063202	0.0
12	0.0	0.0	0.0	4.2087434100235255	0.0
13	0.0	0.0	0.0	4.387192142708133	0.0
14	0.0	0.0	0.0	4.4775144772891045	0.0
15	5.7787802035170815E-5	0.0	0.0	4.547957807969978	0.0
16	5.7787802035170815E-5	0.0	0.0	4.6605284463344905	0.0
17	5.7787802035170815E-5	0.0	0.0	4.798005627376162	0.0
18	5.7787802035170815E-5	0.0	0.0	5.012860675342927	0.0
19	5.7787802035170815E-5	0.0	0.0	5.098906712573297	0.0
20	5.7787802035170815E-5	0.0	0.0	5.200324305145021	0.0
21	5.7787802035170815E-5	0.0	0.0	5.322256567439232	0.0
22	5.7787802035170815E-5	0.0	0.0	5.460889504521607	0.0
23	5.7787802035170815E-5	0.0	0.0	5.619054718691869	0.0
24	5.7787802035170815E-5	0.0	0.0	5.747863729428265	0.0
25	5.7787802035170815E-5	0.0	0.0	5.854019921766874	0.0
26	5.7787802035170815E-5	0.0	0.0	5.96104293113601	0.0
27	5.7787802035170815E-5	0.0	0.0	6.066967972266478	0.0
28	5.7787802035170815E-5	0.0	0.0	6.175377888884459	0.0
29	5.7787802035170815E-5	0.0	0.0	6.287255073624549	0.0
30	5.7787802035170815E-5	0.0	0.0	6.453972882496017	0.0
31	5.7787802035170815E-5	0.0	0.0	6.582435166420201	0.0
32	5.7787802035170815E-5	0.0	0.0	6.70511867014087	0.0
33	5.7787802035170815E-5	0.0	0.0	6.826010751998447	0.0
34	5.7787802035170815E-5	0.0	0.0	6.950890192196451	0.0
35	5.7787802035170815E-5	0.0	0.0	7.093915002233499	0.0
36	5.7787802035170815E-5	0.0	0.0	7.227462612736779	0.0
37	5.7787802035170815E-5	0.0	0.0	7.362223767082797	0.0
38	5.7787802035170815E-5	0.0	0.0	7.50363051866286	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTAG	30	2.5284626E-6	44.0	36
CCCGTAG	20	7.8575866E-4	44.0	35
TGTTACG	25	4.4437766E-5	44.0	1
TATTGCG	140	0.0	42.428574	1
ACGGGTA	105	0.0	41.90476	5
CGTTTTT	5585	0.0	41.67592	1
CAACGCG	75	0.0	41.066666	1
AGTTGCG	55	7.8216544E-11	40.000004	1
ACGATTG	45	2.3521352E-8	39.11111	1
CATAGCG	90	0.0	39.11111	1
TATCGCG	85	0.0	38.82353	1
AGGGCGA	1640	0.0	38.634148	6
TAGCGCG	40	4.1261592E-7	38.5	1
AGTAACG	40	4.1261592E-7	38.5	1
CGACAGG	115	0.0	38.26087	2
CGACCCG	75	0.0	38.13333	32
TATAGCG	75	0.0	38.13333	1
TAGGGAC	1940	0.0	37.989693	5
CTCCGAC	35	7.2923394E-6	37.714287	17
CGCGATT	35	7.2923394E-6	37.714287	29
>>END_MODULE